WormMine: ExpressionPattern Expr3697

• Pattern: Fluorescence was observed in epithelial cells that synthesize cuticle. The gfp fusion gene for the exoribonuclease xrn-2 was expressed in specialized myoepithelial cells that secrete the pharyngeal cuticle. Expression of xrn-2p::gfp in the pharyngeal myoepithelium intensified prior to molting. xrn-2p::gfp was expressed in several anterior neurons, including sensory neurons, as well as the PVT neuron that projects along the ventral cord, and the M5 pharyngeal neuron. Also, xrn-2p::gfp was expressed in M5 only in larvae. The xrn-2 reporter was also expressed in the intestine. However, expression of xrn-2p::gfp in the intestine persisted in adults that no longer molt. Expression of the gfp fusion genes was never detected in the hypodermis of gravid adults that no longer molt.
• Primary Identifier: Expr3697

4 Anatomy Terms

Definition	Name	Synonym	Primary Identifier
A chain of very large cuboidal cells forming a wide central lumen in which food arrives from the posterior pharynx, is digested, and from which waste products proceed to the rectum. Intestinal rings form in groups of two and four cells surrounding the common lumen; thus the epithelium is only one cell deep at any point, with neighboring cells firmly secured to their neighbors by apical adherens junctions. These cells have very large nuclei and many large vacuoles, yolk granules, and other inclusions; the latter increase in number and electron density as the animal ages.	intestine	gut	WBbt:0005772
Neuron class of one interneuron, projects along ventral cord to ring.	PVT	lineage name: ABplpappppa	WBbt:0004070
Neuron class of one pharyngeal motorneuron.	M5 neuron	lineage name: MSpaaapap	WBbt:0004465
cell that makes up epithelium in pharynx.	pharyngeal epithelial cell	pharyngeal epithelium	WBbt:0005459

1 Genes

WormBase Gene ID	Gene Name	Sequence Name	Organism
WBGene00006964	xrn-2	Y48B6A.3	Caenorhabditis elegans

6 Life Stages

Remark	Definition	Other Name	Public Name	Primary Identifier
	The stage that begins when a C.elegans individual is fully-developed and has reached maturity.		adult Ce	WBls:0000041
	The third stage larva. At 25 Centigrade, it ranges 32.5-40 hours after fertilization, 18.5-26 hours after hatch.		L3 larva Ce	WBls:0000035
	The second stage larva. At 25 Centigrade, it ranges 25.5-32.5 hours after fertilization, 11.5-18.5 hours after hatch.		L2 larva Ce	WBls:0000027
	The fourth stage larva. At 25 Centigrade, it ranges 40-49.5 hours after fertilization, 26-35.5 hours after hatch.		L4 larva Ce	WBls:0000038
	The first stage larva. At 25 Centigrade, it ranges 14-25.5 hours after fertilization, 0-11.5 hours after hatch.		L1 larva Ce	WBls:0000024
	The whole period of embryogenesis in the nematode Caenorhabditis elegans, from the formation of an egg until hatching.		embryo Ce	WBls:0000003