WormMine: ExpressionPattern Expr9700

• Primary Identifier: Expr9700
• Subcellular Localization: CED-4 does not colocalize with mitochondria in germ cells. In addition, we found no embryonic cells where CED-4::GFP staining colocalized with mitochondria, and in all cells where CED-4 was detectable it localized around the nucleus. We found the same perinuclear CED-4::GFP localization by directly detecting CED-4 by GFP fluorescence in embryos, larvae and adult worms. CED-4 is expressed in the entire germ line, located primarily around the nucleus with additional much weaker granular structures occurring in the cytoplasm. We confirmed the perinuclear staining pattern we observed with a third independently generated CED-4 antibody, which specifically recognizes CED-4. The same predominantly perinuclear CED-4 localization pattern was observed following detection of the CED-4::GFP fusion protein.

1 Anatomy Terms

Definition	Name	Synonym	Primary Identifier
cell line which early in development becomes differentiated from the remaining somatic cell line, and alone has the potential to undergo meiosis and form gametes.	germ line	germline	WBbt:0005784

1 Genes

WormBase Gene ID	Gene Name	Sequence Name	Organism
WBGene00000418	ced-4	C35D10.9	Caenorhabditis elegans

3 Life Stages

Remark	Definition	Other Name	Public Name	Primary Identifier
	The stage that begins when a C.elegans individual is fully-developed and has reached maturity.		adult Ce	WBls:0000041
	The whole period of embryogenesis in the nematode Caenorhabditis elegans, from the formation of an egg until hatching.		embryo Ce	WBls:0000003
	A developmental life stage of the nematode Caenorhabditis elegans that occurs from egg hatching until adulthood.		larva Ce	WBls:0000023