WormMine: ExpressionPattern Expr575

• Pattern: In oocytes,1-cell and 2-cell stage embryos, pie-1 mRNA is distributed uniformly. After the 4-cell stage, pie-1 mRNA was lost from somatic blastomeres and remained in the germ lineage. PIE-1 protein was first detected in maturing oocytes, where it is uniformly distributed. PIE-1 level increase during the one cell stage. PIE-1 protein also exists in P1, P2 P3, P4, Z2 and Z3. In P4, PIE-1 is found equally in the P4 daughters (Z2 and Z3) in contrast to earlier divisions in which PIE-1 is localized to the posterior cytoplasm and posterior centrosomes during mitosis.
• Primary Identifier: Expr575
• Remark: early embryo(author) = blastula embryo(curator).
• Subcellular Localization: A subset of PIE-1 molecules are associated with P granules, but there is a diffuse cytoplasmic component of PIE-1 staining in contrast to the punctate P granule pattern. PIE-1 protein is cytoplasmic until the 2-cell stage, after which it is increasingly nuclear. PIE-1 protein distribution in P0, P1, P2 and P3 follows a similar pattern: initially uniform cytoplasmic distribution, followed by asymmetric cytoplasmic localization at the time of P granule migration, and asymmetric cytoplasmic and centralsomal localizations during mitosis.

9 Anatomy Terms

Definition	Name	Synonym	Primary Identifier
cell line which early in development becomes differentiated from the remaining somatic cell line, and alone has the potential to undergo meiosis and form gametes.	germ line	germline	WBbt:0005784
a female haploid germ cell.	oocyte	ovum	WBbt:0006797
Single cell zygote	P0	lineage name: Z	WBbt:0004422
embryonic founder cell	Psub3	lineage name: P0.ppp	WBbt:0006875
embryonic founder cell	Psub1	lineage name: P0.p	WBbt:0006874
embryonic founder cell	Psub2	lineage name: P0.pp	WBbt:0006873
Embryonic founder cell of germ line	Psub4	lineage name: P0.pppp	WBbt:0004421
Germ line precursor cell	Z2	lineage name: P4.p	WBbt:0004576
Germ line precursor cell	Z3	lineage name: P4.a	WBbt:0004575

1 Genes

WormBase Gene ID	Gene Name	Sequence Name	Organism
WBGene00004027	pie-1	Y49E10.14	Caenorhabditis elegans

2 Life Stages

Remark	Definition	Other Name	Public Name	Primary Identifier
	The stage that begins when a C.elegans individual is fully-developed and has reached maturity.		adult Ce	WBls:0000041
	The C. elegans life stage spanning 0-100min after first cleavage at 20 Centigrade. Proliferate from 1 cell to 28 cells. From first cleavage until the start of gastrulation.		blastula embryo Ce	WBls:0000005