|
|
|
Expr4577
|
|
WGA-G and MBK-2 colocalized in the cortex of oocytes in the proximal germline and in the cortex of newly fertilized embryos. Furthermore, cortical WGA-G and MBK-2 punctae were completely coincident at anaphase of meiosis I. MBK-2 localizes to centrosomes and chromosomes by first mitosis, at which point, WGA-G punctae are no longer detected. |
|
|
|
Expr4504
|
MRG-1 is highly enriched in nuclei and concentrated on chromatin. In early embryos, MRG-1 is present in the nuclei of all blastomeres. In late embryos and young larvae, MRG-1 staining is higher in the nuclei of the two primordial germ cells, Z2 and Z3, than in somatic blastomeres. In larvae and adults, MRG-1 staining is seen primarily in the nuclei of germ cells, although it is also faintly visible in the nuclei of several somatic cell types, including intestinal cells. In the adult germ line, all germ nuclei in the mitotic and meiotic regions are stained. These results demonstrate that MRG-1 is present in the germ line at all stages of development and is maternally loaded into embryos. In addition, zygotically expressed MRG-1 is produced in all cells by at least the 100-cell stage; it accumulates to higher levels in the primordial germ cells than in somatic tissues. |
Expressed in nuclei. |
|
early embryo (author) = blastula embryo (curator) --wjc. |
|
Expr1736
|
In early embryos, MES-3 protein is present in the nuclei of all cells. As embryogenesis progresses, staining gradually diminishes in somatic cells. In late embryos and L1 larvae, MES-3 is detectable in some somatic cells but is most prominent in Z2 and Z3, the primordial germ cells. The nuclear staining of MES-3 is reduced below detection in any of the four nonconditional alleles of mes-3. In wild type adults, MES-3 is most prominent in germline nuclei and is occasionally barely detectable in intestinal nuclei. In the germline, it is present at low levels in distal mitotic nuclei, undetectable in the pachytene region of the distal arm, and present at elevated levels in the proximal meiotic region and in oocytes. |
MES-3 is localized predominantly in nuclei. The immunolocalization pattern of MES-3 was analyzed in embryos, using confocal microscopy. Cells at different stages of mitosis were stained by affinity-purified anti-MES-3 antibody and anti-penta-acetylated H4 antibody to visualize chromosomes. During interphase and prometaphase, when condensed chromosomes are clearly visible in nuclei, MES-3 protein is not obviously concentrated on chromosomes; instead it appears evenly distributed in the nucleoplasm. During metaphase and early anaphase, when nuclear envelopes are broken down, some MES-3 protein is detectably associated with chromosomes. |
|
|
|
Expr2579
|
SCC-1/COH-2 was expressed in germ cells throughout the development, including the adult stage. SCC-1/COH-2 was detected in virtually all mitotic germ nuclei. Similarly to somatic cells in embryos, SCC-1/COH-2 was dispersed in the cytoplasm at mitotic prometaphase and was absent from the condensed anaphase chromosomes in germ cells. In female germ cells that entered meiotic prophase in adult hermaphrodites, SCC-1/COH-2 was observed uniformly in the nuclei. It was unclear whether SCC-1/COH-2 localized to the condensed meiotic chromosomes, because of the strong SCC-1/COH-2 signal emitted from the nucleoplasm. SCC-1/COH-2 was detected also in male germ cells at mitosis and meiosis, but it was not detectable in mature sperm. SCC-1/COH-2 was strongly expressed in virtually all cells in early embryos, but its expression was gradually weakened, and the signal could hardly be detected in late embryos, in which cell division was ceased almost completely. Strong nuclear signals of SCC-1/COH-2 reappeared in larvae, though they were limited to a subset of cells. SCC-1/COH-2 was detectable only in cells that were going to divide. For example, in an L1 larva, intense SCC-1/COH-2 signals were detected in the 14 hypodermal V lineage cells, which divide synchronously. The SCC-1/COH-2 signal was dispersed and not detectable on condensed chromosomes, as observed in embryos of an intermediate stage. In a slightly older L1 larva, expression of SCC-1/COH-2 was seen in 22 P lineage cells to constitute the ventral nerve cord and in four Q lineage cells to produce posterior neuronal cells, all of which divide at the same time. In this L1 larva, no signal was detected in the V lineage cells, suggesting that the SCC-1/COH-2 protein is present only for a short time in the cell cycle, and likely to be degraded quickly after cell division. Larvae of later stages also expressed SCC-1/COH-2 in dividing cells: in an L3 larva, SCC-1/COH-2 was detected in four M lineage cells to produce the uterine and vulval muscle cells and in 10 P lineage vulval precursor cells, which divide concurrently. The embryos were stained with both anti-SCC-1/COH-2 antibodies and an antibody against a component of the nuclear pore complexes. The SCC-1/COH-2 signal was evenly distributed within the nuclear envelope except for the chromosomal region, suggesting that SCC-1/COH-2 molecules dissociated from the chromosomes at metaphase were trapped by the nuclear envelope. Consistently with this interpretation, the SCC-1/COH-2 staining around the metaphase plate was no longer seen at later stages of embryogenesis involving >30 cells, where nuclear envelope is known to break down before metaphase. SCC-1/COH-2 was dispersed into the whole cytoplasm of metaphase cells at these stages. |
SCC-1/COH-2 seemed to localize to the chromosomes in a cell cycle-dependent manner. In interphase, SCC-1/COH-2 was seen throughout the nucleus, overlapping largely with DNA. At mitotic prophase, SCC-1/COH-2 started to separate from condensing chromosomes, and it was not detected on the chromosomes at prometaphase and metaphase. At metaphase, the SCC-1/COH-2 signal seemed as if surrounding the metaphase plate, although it was possible that a small amount of SCC-1/COH-2 was remaining on the metaphase chromosomes but escaped detection, because cohesin is reported to become detectable on metaphase chromosomes only after detergent extraction of soluble background in other metazoans. The SCC-1/COH-2 signal was then dispersed in the cytoplasm at anaphase. At telophase, the SCC-1/COH-2 protein began to reaccumulate on the chromosomes. |
|
Picture: Fig. 5. The same pattern was seen with two separate antibodies raised against distinct PLP-1 peptides, and both nuclear and P granule expression was largely eliminated in plp-1 (RNAi) embryos, confirming their specificity. |
|
Expr8706
|
Immunoreactive PLP-1 localizes to the nuclei of all blastomeres beginning by the two-cell stage of embryogenesis, implying that PLP-1 is a maternally encoded transcription factor. It is also present in the germline-specific P granules of early embryos. |
PLP-1 is transiently asymmetrically localized during telophase of the dividing EMS cell (observed in 12 embryos at the correct stage), with higher levels of the protein in the chromatin of the future E cell nucleus and low or undetectable levels in that of MS. A similar transient asymmetry in PLP-1 levels was observed at many divisions throughout early development, starting at cleavage of the zygote, with higher levels seen in the cytoplasm and forming nucleus of the posterior daughter, P1 (observed in 5 embryos). The anteroposterior asymmetry in PLP-1 was also observed in the AB lineage during the division of the AB granddaughters (observed in 7 embryos): for example, PLP-1 is higher in the chromatin of the posterior daughter ABalp than that of its anterior sister ABala. In all cases, the asymmetry was observed only during telophase and at the time that nuclei were reassembling after cell division; the staining was symmetric at all other times. PLP-1 was always seen at higher levels in the forming nuclei of the posterior daughters. |
|
early embryo(author) = blastula embryo(curator). |
|
Expr584
|
gld-1 mRNA is contained in all blastmeres of embryos with 8 or fewer cells. Subsequently, gld-1 mRNA disappears rapidly from somatic blastmeres and is only detected in the germ lineage. By the 16 cell stage, gld-1 mRNA is only detected in P3. |
|
|
|
|
Expr2551
|
In situ hybridization analysis revealed that spn-4 mRNA was abundant in early embryos. The mRNA was present at the same level in all blastomeres up to the 4-cell stage. Afterwards, it persists in the P blastomere and its sister, and then just the germ lineage. The mRNA was also present in the adult gonads. |
|
|
|
|
Expr2947
|
In late embryos (after the comma stage) matefin staining decreased in all somatic cells but intensified in the nuclear envelopes of the two primordial germ cells, Z2 and Z3. The identity of Z2 and Z3 cells was verified by double labeling with antibodies against PGL-1, which is specific to germ cells. Throughout larva stages L1-L4 and in adults, matefin was present only in germ cells. Matefin signal declined during spermatogenesis and was undetectable in sperm. |
Matefin was detected at the nuclear envelope of all early embryonic cells. |
|
early embryo(author) = blastula embryo(curator). |
|
Expr572
|
SKN-1 protein first becomes visible in oocyte and sperm pronuclei before the first mitotic division of the zygote. SKN-1 becomes cytoplasmic as AB and P1 enter mitosis. P2 and EMS have more SKN-1 than AB daughters. By the 8-cell stage, the granddaughters of AB do not stain for SKN-1, but P1 granddaughters do. SKN-1 is not detectable by the 12-cell stage. |
After the first cleavage, SKN-1 protein locates at the nuclei of AB and P1. As AB and P1 enter mitosis, SKN-1 protein is distributed throughout cytoplasm. |
|
Maternal effect. early embryo(author) = blastula + gastrulating embryo(curator). |
|
Expr576
|
In adult hermaphrodites, mex-1 mRNA is detected in the syncytial core of the gonad and in oocytes at all stages of maturation. In 1 and 2-cell stage embryos, mex-1 mRNA is distributed uniformly, but then appears to be degraded rapidly in somatic blastomeres but remains in germ line blastomeres in subsequent divisions. After P4 divides, mex-1 mRNA is not detected. |
|
|
See Expression pattern 546 for distribution of APX-1 protein. early embryo(author) = blastula embryo(curator). |
|
Expr545
|
Between the newly-fertilized 1-cell stage and the 8-cell stage, apx-1 mRNA is present in all blastomeres at equivalent levels. After the 8-cell stage, apx-1 mRNA rapidly disappears from somatic blastomeres; in 12-cell stage embryos, apx-1 mRNA is visible in the P3 blastomere, but disappears from MS and all other blastomeres. In the 36-cell stage and later embryos, apx-1 mRNA was detected in one to five unidentified nuclei. |
|
|
|
|
Expr2575
|
In situ hybridization analysis revealed that spn-4 mRNA was abundant in early embryos. The mRNA was present at the same level in all blastomeres up to the 4-cell stage. Afterwards, it persists in the P blastomere and its sister, and then just the germ lineage. The mRNA was also present in the adult gonads. |
|
|
|
|
Expr15824
|
MIP-1 is omnipresent throughout development, localizing exclusively to the germline precursor lineage (from P0 through P4) and remaining prominent in the germline pro- genitor cells Z2 and Z3. In contrast, MIP-2 begins to dissipate in P3 and is virtually undetectable when Z2 and Z3 are born. |
|
|
early embryo(author) = blastula embryo(curator). |
|
Expr574
|
PIE-1 protein is localized to the posterior of the zygote and continues to be observed in the germ line P1 through P4 and Z2 and Z3, but not in somatic blastomeres. |
PIE-1 is associated with P granules in germline blastomere cytoplasm. At mitosis, PIE-1 accumulates around the centrosomes of the spindle and PIE-1 decreases in the cytoplasm. PIE-1 staining remains only in the germline blastomeres after division. After P4 divides, PIE-1 persists in both Z2 and Z3 centrosomes. |
|
early embryo(author) = blastula embryo(curator). |
|
Expr575
|
In oocytes,1-cell and 2-cell stage embryos, pie-1 mRNA is distributed uniformly. After the 4-cell stage, pie-1 mRNA was lost from somatic blastomeres and remained in the germ lineage. PIE-1 protein was first detected in maturing oocytes, where it is uniformly distributed. PIE-1 level increase during the one cell stage. PIE-1 protein also exists in P1, P2 P3, P4, Z2 and Z3. In P4, PIE-1 is found equally in the P4 daughters (Z2 and Z3) in contrast to earlier divisions in which PIE-1 is localized to the posterior cytoplasm and posterior centrosomes during mitosis. |
A subset of PIE-1 molecules are associated with P granules, but there is a diffuse cytoplasmic component of PIE-1 staining in contrast to the punctate P granule pattern. PIE-1 protein is cytoplasmic until the 2-cell stage, after which it is increasingly nuclear. PIE-1 protein distribution in P0, P1, P2 and P3 follows a similar pattern: initially uniform cytoplasmic distribution, followed by asymmetric cytoplasmic localization at the time of P granule migration, and asymmetric cytoplasmic and centralsomal localizations during mitosis. |
|
early embryo(author) = blastula + gastrulating embryo(curator). |
|
Expr578
|
Antibody staining is first detected at the bend of the reflexed hermaphrodite gonad. PAR-1 is seen in newly formed, but not mature oocytes nor newly fertilized zygotes. PAR-1 reappears in the late zygote, when both pronuclei are decondensed and the female pronucleus is just starting to migrate toward the posterior. In P0 through P3, it is localized in the posterior periphery so that it is distributed to the germ line blastomeres. When P4 divides, PAR-1 is distributed evenly and distributed to both Z2 and Z3. After P4 divides, PAR-1 gradually fades until it disappears at morphogenesis. |
PAR-1 is membrane-associated. In dividing cells, it is restricted to the posterior until P4 divides, at which time it is located throughout the periphery. PAR-1 co-localizes with P granules. |
|
Antibodies against the amino and carboxy termini gave the same results. early embryo(author) = blastula + gastrulating embryo(curator). |
|
Expr579
|
PAR-1 is found in the cortex of germ line cells in the whole adult gonad. In early embryos, cortical PAR-2 expression continues in P0, P1, P2, P3 and P4 (the cells of germline lineage), fades in Z2 and Z3. In all but P4, PAR-1 exhibits an asymmetric distribution. |
In the distal gonad, PAR-2 staining is strongest at septa between nuclei. In the proximal gonad, PAR-2 is found uniformly in the periphery. PAR-2 is associated with the cortical cytoskeleton. PAR-2 becomes localized to the posterior cortex in P0 through P3. During the symmetrical division of P4, PAR-2 is uniformly distributed at the cortex so that both Z2 and Z3 initially show PAR-2. |
|
Removing the coding region, and using only the Bluescript vector as a probe, does not show staining except a little background. As a negative control, the dpy-20 probe was used for RNA in situ hybridization and no staining in embryos was detected. |
|
Expr1099
|
1-cell, 2-cell and 4-cell embryos show uniform expression pattern. Expression in later embryos and young larvae is predominant in the head region. |
|
|
|
|
Expr2582
|
|
The OMA-1::GFP fluorescence in the developing oocytes and one-cell embryos recapitulated the wild-type spatial and temporal patterns of OMA-1 antibody staining. The punctate staining appeared more pronounced and resembled the characteristic pattern of germline P granules. Starting with the onset of the first mitotic division, the intensity of OMA-1-GFP fluorescence rapidly decreased, and by the time the division was complete, only approximately 10% remained. Interestingly, that remaining 10% of the GFP signal in the two-cell embryo was predominantly found in the germline precursor, P1, associated with what appeared to be P granules. The GFP signal continued to decrease in two-cell embryos and again was asymmetric after the next division, with most of the remaining fluorescence segregated to P2, where it was also predominantly associated with granules. The OMA-1-GFP signal became too weak to detect in the embryo after the four-cell stage. |
|
No detailed description for cellular expression pattern at later developmental stages. |
|
Expr1294
|
Expression scored from zygote til 50 cell embryo |
Antibody staining is detectable throughout the cytoplasm and at the periphery of blastomeres up to about the 50-cell stage. The peripheral staining is asymmetric in the cells of the germline lineage P0, P1, P2 and P3. Asymmetric peripheral localization of PAR-6 develops as the newly fertilized egg, P0, progresses through the cell cycle. Localized antibody staining is not detectable in unfertilized mature oocytes, nor in newly fertilized eggs undergoing the first meiotic division. Patchy PAR-6 staining was first detected at the periphery of the embryo after completion of meiosis I, as determined by the presence of a single polar body. 70% of embryos in meiosis II exhibit patchy PAR-6 staining all around the periphery with no obvious asymmetry (n=20). When the female pronucleus completes metaphase II as indicated by the presence of a second polar body, peripheral PAR-6 is restricted to the anterior 85% of embryo length. In embryos between prophase and telophase of the first mitosis, the peripheral PAR-6 is restricted to the anterior 55% EL of the embryos (n=24). During the first cleavage PAR-6 staining is detectable in the advancing furrow. Asymmetry of PAR-6 is also observed in P1 and P2, the germline cells in 2-cell and 4-cell embryos, respectively. Just after the first cleavage, strong peripheral staining is present where P1 apposes AB. The strength of this signal suggests that protein is present along this boundary in both cells. As the P1 cell progressed into prophase, PAR-6 distribution extends posteriorly, covering the anterior 30% of 13 of 16 P1 cells scored. Starting in late metaphase and through anaphase, the distribution of PAR-6 in P1 becomes skewed to the ventrolateral periphery, the part of the cell fated to give rise to EMS. PAR-6 is distributed uniformly at the AB periphery throughout the 2-cell stage. In the 4-cell stage, PAR-6 peripheral staining is strong in ABa, ABp and EMS, but PAR-6 can be detected only faintly around the periphery of P2 until metaphase of P2 at the 7-cell stage. At this time PAR-6 is asymmetrically localized to the dorsal side of P2, the part of the cell fated to give rise to C. The protein is distributed asymmetrically in P3 as well. Peripheral PAR-6 could be detected till about the 50-cell stage. |
|
|
|
Expr3682
|
|
WRM-1::GFP levels were high in the nucleus of the E blastomere at the eight-cell stage. However, a time course of images revealed that WRM-1::GFP is at first localized similarly in both newly formed nuclei at the beginning of telophase of the EMS cell division. Furthermore, even signal-nonresponsive cells, including cells present at the one-, two- and four-cell stages, exhibit nuclear accumulation of WRM-1 at the beginning of telophase. During the EMS cell division, the nuclear signal in MS grows weaker, while in E, the nuclear WRM-1::GFP levels become higher. A high WRM-1::GFP nuclear level during and after cytokinesis was only observed in signal-responding cells, including numerous posterior descendants of later a/p divisions. In addition to its change in nuclear levels, a cell cycle- and signal-dependent change in the localization of WRM-1 protein was also observed at the cell cortex and cell-cell contact sites. |
|
This information was extracted from published material (Archana Sharma-Oates, Andrew Mounsey and Ian A. Hope). |
|
Expr746
|
pal-1 RNA evenly distributed in 1- and 2-cell embryos. In 4-cell embryos, pal-1 RNA was either uniformly distributed (approximately 50%) or preferentially localized to the posterior blastomere. Nuclear localized transcripts were not detected before the 24-cell stage. In 6 cell and older embryo, pal-1 RNA was more abundant in cells that express PAL-1 protein. |
|
|
A granular staining pattern was found for SPN-4 in the germline blastomeres. This pattern exactly matched the granular staining pattern for POS-1, suggesting that these structures corresponded to P granules. Indeed, the granular patterns matched exactly those observed with a P-granule-specific antibody, indicating that SPN-4 is a component of P granules. The specificity of the antibody was confirmed by the fact that SPN-4 was detected neither in the deletion mutant tm291 nor in spn-4(RNAi) embryos. The distribution of SPN-4 overlapped with that of POS-1 in the posterior blastomeres. |
|
Expr2552
|
Immunostaining revealed that the SPN-4 protein was already present in oocytes. The protein was abundant in one-cell-stage embryos. At the two-cell stage, it was present with equal abundance in both blastomeres AB and P1. At the four-cell stage, the protein distribution became different among the blastomeres; strongly in the posterior blastomeres, EMS and P2, and weakly in the anterior blastomeres, ABa and ABp. After the four-cell stage, SPN-4 was localized to the posterior blastomeres, including the germline precursors, and became undetectable by mid-embryogenesis. |
|
|
|
|
Expr3781
|
|
These antibodies detect a strong and uniform signal at the cell cortex of early embryos. This distribution corresponds to bona fide GPA-16 because it is significantly diminished in gpa-16(RNAi) embryos. These antibodies also label the cytoplasm, with a slight enrichment in the vicinity of microtubule asters, but this aspect of the signal is barely diminished in gpa-16(RNAi) embryos, suggesting that it is not specific or corresponds to a particularly stable pool of GPA-16. In summary, GPA-16 is present predominantly at the cortex of one-cell stage embryos. |
|
|
|
Expr12267
|
The transcript of the uncharacterized open reading frame W02F12.3 displays a suggestive distribution, with a bias towards the anterior of the zygote and of embryos in subsequent stages of embryogenesis. Asymmetric enrichment of era-1 mRNA becomes apparent during prophase of the first cell cycle. Moreover, era-1 mRNA is enriched in the anterior blastomere AB compared to the posterior blastomere P1 in two-cell embryos. This difference persists when comparing the AB daughters ABa and ABp with the P1 daughters EMS and P2. No signal is detected in era-1(RNAi) embryos, indicating probe specificity. The transcript of the uncharacterized open reading frame W02F12.3 displays a suggestive distribution, with a bias towards the anterior of the zygote and of embryos in subsequent stages of embryogenesis.Asymmetric enrichment of era-1 mRNA becomes apparent during prophase of the first cell cycle. Moreover, era-1 mRNA is enriched in the anterior blastomere AB compared to the posterior blastomere P1 in two-cell embryos. This difference persists when comparing the AB daughters ABa and ABp with the P1 daughters EMS and P2. No signal is detected in era-1(RNAi) embryos, indicating probe specificity. |
|
|
early embryo(author) = 1-cell + 2-cell + 4-cell embryo(curator). |
|
Expr573
|
mex-3 mRNA is detected in the syncytial core of gonad arm and distribute uniformly in oocyte and early 1-cell embryo. mex-3 mRNA is more abundant in AB and its daughter cells than P1 and its daughter cells. After 4-cell stage, mex-3 mRNA disappears. MEX-3 protein is present in oocytes and uniformly distributed until the first division, when they become more abundant in the anterior AB cell. At the 4-cell stage, AB daughters have more MEX-3. Both the mRNA and protein disappear after the 4-cell stage. |
MEX-3 is cytoplasmic, and in the P cells, MEX-3 is associated with P granules. |
|
early embryo (author) = blastula embryo (curator) --wjc. |
|
Expr1735
|
OOC-5 localization was observed in all cells of early wild-type embryos, in a pattern that changed with the cell cycle. |
In early one-cell interphase and prophase embryos, OOC-5 was enriched around the rim of both pronuclei, as well as being dispersed throughout the cytoplasm in a slightly reticular pattern. A similar distribution was seen in two-cell interphase and prophase embryos. Double labeling with antibodies against tubulin showed that regions of more concentrated OOC-5 staining corresponded to the areas of the microtubule asters. During metaphase in one- and two-cell embryos, OOC-5 remained perinuclear and was also associated with the poles of the mitotic spindle. Upon onset of anaphase, OOC-5 localized on the entire mitotic spindle. The transition from perinuclear OOC-5 localization to general spindle localization coincides precisely with nuclear envelope breakdown in C. elegans. Similar patterns of OOC-5 localization were observed in four-cell embryos, depending on the stage of the cell cycle. In addition, OOC-5 was enriched at the regions of cell-cell contact in some two-cell embryos (40%, n=41) and virtually all four-cell embryos examined (96%, n=24). |
|
|
|
Expr9127
|
|
Expressed in the cytoplasm with increasing levels in growing oocytes, peaking in the oocyte undergoing maturation. The level of OMA-1 protein remain very high following fertilization as well as throughout the first mitotic cycle. The antibody staining often included a slight punctate pattern of fluorescence. Immediately after the first mitotic division, the levels of OMA-1 protein rapidly decreased and became difficult to detect with antibody staining. |
|
The fact that NOS-2 can be detected in the germ lineage as early as the 28-cell stage strongly suggest that NOS-2 is expressed from maternal RNA, since zygotic transcription is not thought to begin in the germ lineage until the 100-cell stage. The specificity of antibody was confirmed by staining embryos deficient for NOS-2. Affinity-purified anti-NOS-2 antibody detected NOS-2 in nos-1(RNAi) and nos-1(gv5) embryos, but not in nos-2(RNAi) embryos. NOS-1 and NOS-2 thus do not depend on each other for expression. |
|
Expr1161
|
NOS-2 protein is expressed sequentially during embryogenesis. First detected expression of NOS-2 in the 1-cell stage; at this stage, NOS-2 was present uniformly throughout the embryo. No NOS-2 expression was detected in 2- to 20-cell embryos. NOS-2 expression reappeared in the 28-cell stage in the cytoplasm of the germline blastomere P4. In some embryos, NOS-2 staining appeared to be concentrated in a few perinuclear foci. NOS-2 expression continued in P4 and its two daughters Z2 and Z3 until approximately the 200-cell stage. NOS-2 levels decreased sharply in later stages and were undetectable by the 550-cell stage. |
cytoplasm |
|
|
|
Expr2581
|
|
Expressed in the cytoplasm with increasing levels in growing oocytes, peaking in the oocyte undergoing maturation. The level of OMA-2 protein remain very high following fertilization as well as throughout the first mitotic cycle. The antibody staining often included a slight punctate pattern of fluorescence. Immediately after the first mitotic division, the level of OMA-2 proteins rapidly decreased and became difficult to detect with antibody staining. |
