WormMine: ExpressionPattern Expr12428

• Pattern: NEG-1::GFP is asymmetrically localized in the early embryo. NEG-1::GFP was detected in the zygotic nucleus and at equal levels in both nuclei of the two-cell embryo (23 of 23). However, at the four-cell stage, NEG-1::GFP expression was markedly higher in nuclei of the anterior AB blastomeres than in the nuclei of EMS and P2 (31 of 34). Following the four-cell stage, NEG-1::GFP remained high in the granddaughters of the AB blastomere and diminished progressively in subsequent divisions (data not shown). In the adult germline, NEG- 1::GFP was observed in the nuclei of distal germ cells and the nuclei of growing oocytes except for the most proximal oocyte. Moreover, intense sub-nuclear localization of NEG-1:GFP was observed on condensed chromatin.
• Primary Identifier: Expr12428

6 Anatomy Terms

Definition	Name	Synonym	Primary Identifier
cell line which early in development becomes differentiated from the remaining somatic cell line, and alone has the potential to undergo meiosis and form gametes.	germ line	germline	WBbt:0005784
embryonic cell	ABa		WBbt:0005906
embryonic cell	ABp		WBbt:0005994
Embryonic founder cell	AB	AB blastomere	WBbt:0004015
embryonic founder cell	Psub1	lineage name: P0.p	WBbt:0006874
A cell that is the AB blast cell or is a lineal descendant of AB.	AB lineage cell		WBbt:0008611

1 Genes

WormBase Gene ID	Gene Name	Sequence Name	Organism
WBGene00017985	neg-1	F32D1.6	Caenorhabditis elegans

3 Life Stages

Remark	Definition	Other Name	Public Name	Primary Identifier
	The stage that begins when a C.elegans individual is fully-developed and has reached maturity.		adult Ce	WBls:0000041
	The C. elegans life stage spanning 0-20min after first cleavage at 20 Centigrade. Contains 2 cells.		2-cell embryo Ce	WBls:0000007
	The C. elegans life stage spanning 20-40min after first cleavage at 20 Centigrade. Contains 4 cells.		4-cell embryo Ce	WBls:0000008