WormMine: ExpressionPattern Expr11037

• Primary Identifier: Expr11037
• Subcellular Localization: Consistent with the transcriptional GFP reporter ROL-3::GFP is expressed in the major hypodermal cells and excluded from seam cells. At the point when seam cells are quiescent ROL-3::GFP expression is generally diffuse across the surface of the major hypodermis. Prior to and during seam cell division however, ROL-3::GFP expression intensifies and accumulates in foci at the seam cell boundaries. ROL-3::GFP localization at the seam cell boundary is lost at the anterior seam cells as they fuse with the major hypodermis. Particularly intense ROL-3::GFP foci are observed at the leading edges of the posterior seam cells as they elongate towards one another. ROL-3 localization at the seam cell boundary persists until seam cell elongation is completed. In addition to the major hypodermal cells ROL-3::GFP expression is also detected in the anterior seam cells as they take on the major hypodermal fate at the L1 stage of development. These cells migrate to separate the vulval precursor cells (VPCs) which also express ROL-3::GFP at this stage. Expression of ROL-3::GFP in the VPC's is restricted to L1 larval stage, being rapidly reduced during the L1-L2 transition and not detected at latter stages of development.

3 Anatomy Terms

Definition	Name	Synonym	Primary Identifier
Epidermal layer.	hypodermis	epidermis	WBbt:0005733
a group of hypodermal cells that lie along the apical midline of the hypodermis, at the extreme left and right sides between nose and tail	seam cell	lateral hypodermis	WBbt:0005753
cell that has the potential to form structural part of a vulva.	vulval precursor cell	VPC	WBbt:0007809

1 Genes

WormBase Gene ID	Gene Name	Sequence Name	Organism
WBGene00004395	rol-3	C16D9.2	Caenorhabditis elegans

2 Life Stages

Remark	Definition	Other Name	Public Name	Primary Identifier
	The whole period of embryogenesis in the nematode Caenorhabditis elegans, from the formation of an egg until hatching.		embryo Ce	WBls:0000003
	A developmental life stage of the nematode Caenorhabditis elegans that occurs from egg hatching until adulthood.		larva Ce	WBls:0000023