WormMine: ExpressionPattern Expr16525

• Pattern: Consistent with previous publications, GFP::NRDE-3 (bombardment) localizes to the nuclei of somatic cells beginning around 100-cell stage of embryogenesis. No expression can be detected in germ cells or early embryos [Expr16524]. In contrast, GFP::NRDE-3 (CRISPR) is visible in nuclei beginning in late pachytene of the germline and continues to be expressed in oocytes and in all stages of embryogenesis. This discrepancy in expression between the transgenic strain constructed by bombardment and the CRISPR strain suggests that GFP::NRDE-3 (bombardment) may be silenced in the germline and early embryos. In the CRISPR line, it is also expressed in somatic seam cells in L1 larvae.
• Primary Identifier: Expr16525

2 Anatomy Terms

Definition	Name	Synonym	Primary Identifier
a group of hypodermal cells that lie along the apical midline of the hypodermis, at the extreme left and right sides between nose and tail	seam cell	lateral hypodermis	WBbt:0005753
a cell, of either sex, directly concerned in the production of a new organism.	germ cell		WBbt:0006796

1 Genes

WormBase Gene ID	Gene Name	Sequence Name	Organism
WBGene00019862	nrde-3	R04A9.2	Caenorhabditis elegans

2 Life Stages

Remark	Definition	Other Name	Public Name	Primary Identifier
	The first stage larva. At 25 Centigrade, it ranges 14-25.5 hours after fertilization, 0-11.5 hours after hatch.		L1 larva Ce	WBls:0000024
	The whole period of embryogenesis in the nematode Caenorhabditis elegans, from the formation of an egg until hatching.		embryo Ce	WBls:0000003