WormMine: ExpressionPattern Expr2915

• Pattern: daf-9::GFP is expressed in a pair of cells in the anterior ganglion in L1 larvae, which persists in all larval stages and in adults, in the hypodermis from the mid-L2 stage to the end of L4 stage, and in the spermatheca of adult hermaphrodites. The daf-9::GFP-expressing head cells have been identified as XXXL/R, which are thought to be embryonic hypodermal cells.
• Primary Identifier: Expr2915
• Remark: When this transgene was introduced into daf-2(e1370) and daf-7(e1372) mutant animals grown at the non-permissive temperature (25 centigrades), hypodermal expression of daf-9::GFP was absent in dauers, even though the daf-9::GFP expression in XXXL/R persisted. The lack of hypodermal daf-9::GFP expression was also noted in wild-type dauer animals derived from starvation. The daf-9::GFP transgene was unable to suppress dauer arrest of daf-2(e1370) and daf-7(e1372) animals (n>100) at the restrictive temperature (25 centigrades) and enhanced dauer arrest of these animals at the permissive temperature (15 centigrades).

5 Anatomy Terms

Definition	Name	Synonym	Primary Identifier
an accordion-like tube that contains sperm and is the site of oocyte fertilization.	spermatheca		WBbt:0005319
Epidermal layer.	hypodermis	epidermis	WBbt:0005733
Embryonic head hypodermal cell	XXXR	lineage name: ABarpappaa	WBbt:0004579
Embryonic head hypodermal cell	XXXL	lineage name: ABplaaapaa	WBbt:0004581
ganglion anterior to the nerve ring	anterior ganglion		WBbt:0005375

1 Genes

WormBase Gene ID	Gene Name	Sequence Name	Organism
WBGene00000905	daf-9	T13C5.1	Caenorhabditis elegans

6 Life Stages

Remark	Definition	Other Name	Public Name	Primary Identifier
	The stage that begins when a C.elegans individual is fully-developed and has reached maturity.		adult Ce	WBls:0000041
	The third stage larva. At 25 Centigrade, it ranges 32.5-40 hours after fertilization, 18.5-26 hours after hatch.		L3 larva Ce	WBls:0000035
	The second stage larva. At 25 Centigrade, it ranges 25.5-32.5 hours after fertilization, 11.5-18.5 hours after hatch.		L2 larva Ce	WBls:0000027
	The fourth stage larva. At 25 Centigrade, it ranges 40-49.5 hours after fertilization, 26-35.5 hours after hatch.		L4 larva Ce	WBls:0000038
	The first stage larva. At 25 Centigrade, it ranges 14-25.5 hours after fertilization, 0-11.5 hours after hatch.		L1 larva Ce	WBls:0000024
	The whole period of embryogenesis in the nematode Caenorhabditis elegans, from the formation of an egg until hatching.		embryo Ce	WBls:0000003