WormMine: ExpressionPattern Expr16238

• Pattern: We observed low levels of Pina-1::GFP expression in GABAergic motor neurons of both L1 and L4 animals. At the L1 stage, where only DD GABAergic neurons are present, we noted dim fluorescence in DD cell bodies that was obscured by comparatively high levels of Pina-1::GFP epidermal expression. At the L4 stage, somatic fluorescence in GABAergic motor neurons remained dim, but was more clearly discernible in comparison with L1, likely due to decreased Pina-1::GFP expression in the epidermis of L4 stage animals. We noted consistent low levels of expression across both VD and DD GABAergic neurons. We also noted stronger labeling of additional ventral cord motor neurons that were not labeled by the Punc-47::mCherry reporter, suggesting expression of ina-1 in other motor neuron classes. To address this possibility, we generated a transgenic strain co-expressing the Pina-1::GFP reporter with the cholinergic marker Pacr-2::mCherry. Overall, we noted that Pina-1::GFP fluorescence was stronger in cholinergic motor neurons compared with GABAergic motor neurons, and was expressed in only a subset of cholinergic motor neurons at both L1 and L4 stages. At the L4 stage, Pina-1::GFP fluorescence was most consistently observed labeling the B-class cholinergic motor neurons.
• Primary Identifier: Expr16238

22 Anatomy Terms

Definition	Name	Synonym	Primary Identifier
Epidermal layer.	hypodermis	epidermis	WBbt:0005733
neuron that uses GABA as a vesicular neurotransmitter.	GABAergic neuron		WBbt:0005190
neuron that uses vesicular acetylcholine as a neurotransmitter.	cholinergic neuron	ACh neuron	WBbt:0006840
Ventral cord motor neuron, innervates ventral body muscles, probably reciprocal inhibitor	VD13	lineage name: P12.app	WBbt:0004585
Ventral cord motor neurons, reciprocal inhibitors, chnage synaptic pattern during postembryonic development	DD6	lineage name: ABprppapppp	WBbt:0004824
Ventral cord motor neurons, reciprocal inhibitors, chnage synaptic pattern during postembryonic development	DD2	lineage name: ABprppappap	WBbt:0004838
Ventral cord motor neuron, innervates ventral body muscles, probably reciprocal inhibitor	VD12	lineage name: P11.app	WBbt:0004587
Ventral cord motor neuron, innervates ventral body muscles, probably reciprocal inhibitor	VD11	lineage name: P10.app	WBbt:0004589
Ventral cord motor neuron, innervates ventral body muscles, probably reciprocal inhibitor	VD10	lineage name: P9.app	WBbt:0004591
Ventral cord motor neuron, innervates ventral body muscles, probably reciprocal inhibitor	VD9	lineage name: P8.app	WBbt:0004593
Ventral cord motor neuron, innervates ventral body muscles, probably reciprocal inhibitor	VD7	lineage name: P6.app	WBbt:0004598
Ventral cord motor neuron, innervates ventral body muscles, probably reciprocal inhibitor	VD6	lineage name: P5.app	WBbt:0004599
Ventral cord motor neuron, innervates ventral body muscles, probably reciprocal inhibitor	VD8	lineage name: P7.app	WBbt:0004597
Ventral cord motor neuron, innervates ventral body muscles, probably reciprocal inhibitor	VD4	lineage name: P3.app	WBbt:0004603
Ventral cord motor neuron, innervates ventral body muscles, probably reciprocal inhibitor	VD5	lineage name: P4.app	WBbt:0004601
Ventral cord motor neuron, innervates ventral body muscles, probably reciprocal inhibitor	VD2	lineage name: P1.app	WBbt:0004607
Ventral cord motor neuron, innervates ventral body muscles, probably reciprocal inhibitor	VD3	lineage name: P2.app	WBbt:0004605
Ventral cord motor neuron, innervates ventral body muscles, probably reciprocal inhibitor	VD1	lineage name: W.pp	WBbt:0004609
Ventral cord motor neurons, reciprocal inhibitors, chnage synaptic pattern during postembryonic development	DD4	lineage name: ABprppapppa	WBbt:0004826
Ventral cord motor neurons, reciprocal inhibitors, chnage synaptic pattern during postembryonic development	DD5	lineage name: ABplppapppp	WBbt:0004825
Ventral cord motor neurons, reciprocal inhibitors, chnage synaptic pattern during postembryonic development	DD3	lineage name: ABplppapppa	WBbt:0004837
Ventral cord motor neurons, reciprocal inhibitors, chnage synaptic pattern during postembryonic development	DD1	lineage name: ABplppappap	WBbt:0004839

1 Genes

WormBase Gene ID	Gene Name	Sequence Name	Organism
WBGene00002081	ina-1	F54G8.3	Caenorhabditis elegans

2 Life Stages

Remark	Definition	Other Name	Public Name	Primary Identifier
	The fourth stage larva. At 25 Centigrade, it ranges 40-49.5 hours after fertilization, 26-35.5 hours after hatch.		L4 larva Ce	WBls:0000038
	The first stage larva. At 25 Centigrade, it ranges 14-25.5 hours after fertilization, 0-11.5 hours after hatch.		L1 larva Ce	WBls:0000024