WormMine: AnatomyTerm WBbt:0004585

• Definition: Ventral cord motor neuron, innervates ventral body muscles, probably reciprocal inhibitor
• Name: VD13
• Primary Identifier: WBbt:0004585
• Synonym: lineage name: P12.app

1 Children

Definition	Name	Synonym	Primary Identifier
nucleus of pedigree P12.app	P12.app nucleus		WBbt:0003242

1 Expression Clusters

Regulated By Treatment	Description	Algorithm	Primary Identifier
	Transcripts enriched in VD_DD according to single cell RNAseq.	Genes that pass the Bonferroni threshold for multiple comparisons (q < 0.05) are significantly enriched.	WBPaper00061651:VD_DD_enriched

41 Expression Patterns

• Primary Identifier: Expr15619

• Primary Identifier: Expr15558

• Primary Identifier: Expr15560

• Primary Identifier: Expr9325
• Pattern: Synaptogyrin is expressed in all 26 GABAergic neurons including also RMER and most though not all other neurons. Synaptogyrin is absent in amphids and phasmids and can be detected in non-neuronal glial-like sheath cells in adult worms. The cephalic neurons CEPDR/L and CEPVR/L and amphid-associated sheath cells CEPshDR/L, CEPshVR/L were tentatively positive. Several other neurons that could be tentatively identified in the anterior part are MI, M4, I4, AVL, AIY, RIS, I5, M3R/L, and in the posterior part DVA, AS11, ALNR/L, DVC, DVB, PQR, DA9 (characteristic axonal process denoted by arrowhead), VD13, DD6, VD12. Of these, AVL, RIS, VD13, DD6 and VD12 are GABAergic based on the colocalization with the unc-47p::GFP reporter. In addition, IL neurons were tentatively identified in the anterior (IL*). Synaptogyrin reporter constructs are also expressed in developing neurons. The expression of sng-1p::YFP is closely associated with the development of the nervous system being absent in the gastrula stage with first fluorescence in neuronal precursor cells and newly-formed neurons in the anterior part during the 1.5-fold stage. In addition, it is also detected transiently in cells in the posterior body at the 1.25-fold and 1.5-fold stage.

• Primary Identifier: Expr15567

• Primary Identifier: Expr15571

• Primary Identifier: Expr15572

• Primary Identifier: Expr15573

• Primary Identifier: Expr15579

• Primary Identifier: Expr15586

• Primary Identifier: Expr15651

• Primary Identifier: Expr15652

• Primary Identifier: Expr15589

• Primary Identifier: Expr13158

• Primary Identifier: Expr15591

• Primary Identifier: Expr15598

• Primary Identifier: Expr15604

• Primary Identifier: Expr15608

• Primary Identifier: Expr15611

• Primary Identifier: Expr1734
• Remark: Lines expressing GFP::CED-10 were generated by injection of ced-10::gfp::ced-10 with an unc-76-rescuing construct, P76-16B, both at 75 ng/l, into unc-76(e911) animals.
• Pattern: Cell type identifications were confirmed by the examination of a nuclear-localized GFP expressed from the ced-10 promoter. GFP::CED-10 was expressed broadly, perhaps in all cells, including neurons (CANs, VDs and DDs), engulfing cell types (including the hypodermis, intestine and pharynx) and the distal tip cells. Expression was first seen in early embryogenesis and continued throughout adulthood in all cell types.
• Subcellular Localization: Strong fluorescence was observed within the axons of the nerve ring. The GFP::CED-10 fusion protein accumulated at the plasma membrane.

• Primary Identifier: Expr7808
• Remark: Picture: Figure 7.
• Pattern: A highly reproducible pattern of DKF-1 expression was observed as animals matured from embryo to adult. Intense fluorescence signals corresponding to DKF-1GFP revealed robust kinase accumulation in both (a) a region bounded by the anterior and posterior bulbs of the pharynx and (b) a tail area that contains lumbar, dorsorectal and pre-anal ganglia. Specifically, DKF-1 is differentially enriched in a cluster of cells that are immediately adjacent to the posterior pharyngeal bulb. Strong signals also emanate from cells positioned along the lateral surface of this bulb in animals carrying the dkf-1P::DKF-1GFP transgene. At the anterior pharyngeal bulb, DKF-1 accumulates selectively in bodies and in very thin processes (dendrites and axons) of two neurons. Nearly all cells expressing DKF-1 appear to be neurons. Two fluorescent cells with similar sizes and locations (at the anterior edge of the isthmusposterior bulb) may be M2 motor neurons. The location of the more posterior fluorescent neuron approximates the position of the cell body of an NSM neuron. DKF-1 also accumulates in a cell resembling I1. Other candidate DKF-1-enriched cells in the pharyngeal region include: the AWB, ADL, and ADF chemosensory neurons; and AVB and AIA interneurons.n C. elegans tail, DKF-1GFP expression is differentially elevated in neurons located within the dense neuropile of several tail ganglia. The pattern of fluorescence reveals that cell bodies and/or processes of phasmid neurons (PHA, PHB, and PHC), interneurons (PVC, DVA, DVB, PVQ, PVT) and motor neurons (VD13, DD6, VA12) are candidate sites for accumulation of DKF-1 protein.
• Subcellular Localization: Expressed in neuronal cell bodies and/or processes.

• Primary Identifier: Expr15648

• Primary Identifier: Expr15641

• Primary Identifier: Expr15633

• Primary Identifier: Expr15335

• Primary Identifier: Expr13159

• Primary Identifier: Expr15620

• Primary Identifier: Expr1459
• Remark: Suncellular localization was revealed by another construct with GFP inserted at the carboxy terminus of the UNC-47 protein. This construct rescued the unc-47 mutant phenotype, demonstrating that the construct functions normally.
• Pattern: Animals containing the unc-47::GFP reporter construct showed expression of GFP in all GABAergic neurons, and only in GABAergic neurons.
• Subcellular Localization: Done by another gfp construct: GFP-tagged UNC-47 was localized to synaptic varicosities along the ventral and dorsal cords but not in axons. show that unc-47 expression is associated with synaptic vesicle.

• Primary Identifier: Expr1205
• Remark: Reporter gene fusion type not specified. These expression patterns were identical to those for GABA, unc-47, and unc-25.
• Pattern: expressed in all GABAergic neurons, which include the 19 D neurons in the ventral cord, the four RMEs, AVL, and RIS in the head, and DVB in the tail.

• Primary Identifier: Expr1206
• Remark: Reporter gene fusion type not specified.
• Pattern: expressed in all GABAergic neurons, which include the 19 D neurons in the ventral cord, the four RMEs, AVL, and RIS in the head, and DVB in the tail. These expression patterns were identical to those for GABA, unc-47, and unc-25.

0 Life Stages

3 Parents

Definition	Name	Synonym	Primary Identifier
Neuron class of thirteen motoneurons, with cell bodies in the ventral cord, which innervate ventral muscles. Each cell has an anteriorly directed process emanating from its cell body.	VD neuron		WBbt:0005303
neuron with cell body in the preanal ganglion.	preanal ganglion neuron	preanal ganglion neurons	WBbt:0005447
post-embryonic cell of pedigree P12.ap	P12.ap		WBbt:0007233