WormMine: ExpressionPattern Expr12734

• Pattern: EFF-1 was first detected at the bean stage within individual puncta in the dorsal and ventral hypodermal cells before fusion. Subsequently, EFF-1 appeared as punctate staining in the cytoplasm of hyp6 and hyp7 precursor cells at the comma stage. Following embryonic fusions, EFF-1 remained vesicular, and the number of EFF-1 puncta increased when cell fusion was nearly completed. EFF-1 puncta showed minor co-localization with apical cell junctions detected by anti-DLG-1 antibody. EFF-1 puncta were aligned along longitudinal lines lying parallel to the seam cells. This arrangement might be dictated by the organization of the cytoskeleton in the syncytial hypodermal cells, where actin, intermediate filaments, and microtubules form bundles that run parallel to the seam cells. Thus, EFF-1 is expressed in puncta at the onset, during, and after hypodermal cell fusion in developing embryos.
• Primary Identifier: Expr12734

3 Anatomy Terms

Definition	Name	Synonym	Primary Identifier
Epidermal layer.	hypodermis	epidermis	WBbt:0005733
Cylindrical hypodermal syncytium in head	hyp6	lineage name: ABarpaapaa	WBbt:0004679
Anterior daughter cell of V5L.pppaa, fuses into postembryonic large hypodermal syncytium in male.	V5L.pppaa	lineage name: V5L.pppaa	WBbt:0004223

1 Genes

WormBase Gene ID	Gene Name	Sequence Name	Organism
WBGene00001159	eff-1	C26D10.5	Caenorhabditis elegans

2 Life Stages

Remark	Definition	Other Name	Public Name	Primary Identifier
	The C. elegans life stage spanning 390-420min after first cleavage at 20 Centigrade. Cell number remains at ~560 cells, with some new cells generated and some cells go through programmed cell death. The shape of embryo looks like a comma. A stage between bean embryo and 1.5-fold embryo.		comma embryo Ce	WBls:0000017
	The C. elegans life stage spanning 350-390min after first cleavage at 20 Centigrade. Cell number remains at ~560 cells, with some new cells generated and some cells go through programmed cell death. Emrbyo elongation started but have not formed comma shape yet. The shape of embryo looks like a lima bean. A stage right before comma embryo. Also called lima embryo or lima bean stage.		bean embryo Ce	WBls:0000016