WormMine: ExpressionPattern Expr2637

• Pattern: A second promoter construct corresponding to the 1869 bp immediately upstream of the ATG start codon was also used to generate transgenic worms. This showed a similar pattern of expression in hypodermal cells, and was not expressed in the seam cells. Fluorescence was observed in late embryos and L2d predauer stages in the head hypodermal cells hyp 3, hyp 4, hyp 5, hyp 6, and hyp 7, also hyp 7 along the main body, and in tail hyp 8, hyp 9, hyp 10, and hyp 11. Lateral seam cells did not express cut-6. No expression of gfp was detected in any other stage, either larval or adult. M142.2/cut-6 is therefore expressed in the hypodermal cells. The pattern of spatial and temporal expression of M142.2/cut-6 in the daf-1 and daf-2 mutant backgrounds was identical to that found in the wild type N2 background, confirming that M142.2 was expressed in late embryos and L2d predauers.
• Primary Identifier: Expr2637
• Remark: Reporter gene fusion type not specified.

9 Anatomy Terms

Definition	Name	Synonym	Primary Identifier
entire syncytium of hyp7	hyp7 syncytium	hyp7	WBbt:0005734
Tail ventral hypodermis, 9th hypodermis cell	hyp9		WBbt:0004380
Tail ventral hypodermis	hyp10	lineage name: ABplppppppp	WBbt:0004378
Tail ventral hypodermis, 8th hypodermis cell	hyp8		WBbt:0004381
Cylindrical hypodermal syncytium in head	hyp5	lineage name: ABarpappap	WBbt:0004685
Tail dorsal hypodermis	hyp11	lineage name: Cpappv	WBbt:0004377
Cylindrical hypodermal syncytium in head	hyp3	lineage name: ABplaapaaaa	WBbt:0004690
Cylindrical hypodermal syncytium in head	hyp4	lineage name: ABarpapapa	WBbt:0004687
Cylindrical hypodermal syncytium in head	hyp6	lineage name: ABarpaapaa	WBbt:0004679

1 Genes

WormBase Gene ID	Gene Name	Sequence Name	Organism
WBGene00000853	cut-6	M142.2	Caenorhabditis elegans

2 Life Stages

Remark	Definition	Other Name	Public Name	Primary Identifier
	The whole period of embryogenesis in the nematode Caenorhabditis elegans, from the formation of an egg until hatching.		embryo Ce	WBls:0000003
	A special second stage larva with altered physiology and developmental potential that leads to formation of dauer larva. At 25 Centigrade, it ranges 25.5-32.5 hours after fertilization, 11.5-18.5 hours after hatch.		L2d larva Ce	WBls:0000046