WormMine: ExpressionPattern Expr2552

• Pattern: Immunostaining revealed that the SPN-4 protein was already present in oocytes. The protein was abundant in one-cell-stage embryos. At the two-cell stage, it was present with equal abundance in both blastomeres AB and P1. At the four-cell stage, the protein distribution became different among the blastomeres; strongly in the posterior blastomeres, EMS and P2, and weakly in the anterior blastomeres, ABa and ABp. After the four-cell stage, SPN-4 was localized to the posterior blastomeres, including the germline precursors, and became undetectable by mid-embryogenesis.
• Primary Identifier: Expr2552
• Remark: A granular staining pattern was found for SPN-4 in the germline blastomeres. This pattern exactly matched the granular staining pattern for POS-1, suggesting that these structures corresponded to P granules. Indeed, the granular patterns matched exactly those observed with a P-granule-specific antibody, indicating that SPN-4 is a component of P granules. The specificity of the antibody was confirmed by the fact that SPN-4 was detected neither in the deletion mutant tm291 nor in spn-4(RNAi) embryos. The distribution of SPN-4 overlapped with that of POS-1 in the posterior blastomeres.

10 Anatomy Terms

Definition	Name	Synonym	Primary Identifier
a female haploid germ cell.	oocyte	ovum	WBbt:0006797
Single cell zygote	P0	lineage name: Z	WBbt:0004422
embryonic cell	ABa		WBbt:0005906
embryonic cell	ABp		WBbt:0005994
Embryonic founder cell	AB	AB blastomere	WBbt:0004015
embryonic cell	EMS		WBbt:0006876
embryonic founder cell	Psub1	lineage name: P0.p	WBbt:0006874
embryonic founder cell	Psub2	lineage name: P0.pp	WBbt:0006873
Germ line precursor cell	Z2	lineage name: P4.p	WBbt:0004576
Germ line precursor cell	Z3	lineage name: P4.a	WBbt:0004575

1 Genes

WormBase Gene ID	Gene Name	Sequence Name	Organism
WBGene00004984	spn-4	ZC404.8	Caenorhabditis elegans

2 Life Stages

Remark	Definition	Other Name	Public Name	Primary Identifier
	The stage that begins when a C.elegans individual is fully-developed and has reached maturity.		adult Ce	WBls:0000041
	The C. elegans life stage spanning 0-100min after first cleavage at 20 Centigrade. Proliferate from 1 cell to 28 cells. From first cleavage until the start of gastrulation.		blastula embryo Ce	WBls:0000005