WormMine: ExpressionPattern Expr13301

• Pattern: set-4p::GFP transgenic animals expressed GFP broadly in embryos. Post-embryonically, we detected fluorescence predominantly in the head and tail regions of the animal, in a pattern consistent with neuronal expression. To confirm this, we established a transgenic strain that co-expressed set-4p::GFP and mCherry driven by the pan-neuronal rab-3 promoter (Nonet et al., 1997). At all developmental stages interrogated, we observed co-localization of green and red fluorescence, consistent with somatic set-4p::GFP expression being predominantly neuronal. We did not observe significant GFP expression in intestine, body wall muscle, or hypodermis. The extent of co-localization of green and red fluorescence in set- 4p::GFP transgenic animals exposed to DiI reveals that the set-4 promoter is active in amphid sensory neurons as well as in other cells.
• Primary Identifier: Expr13301

4 Anatomy Terms

Definition	Name	Synonym	Primary Identifier
a cellular object that consists of subcellular components, expresses genes or functions.	Cell	Cell type	WBbt:0004017
neuron with its cell body situated in the head, excluding the pharynx.	head neuron		WBbt:0006751
neuron with its cell body situated in the tail, posterior to rectum.	tail neuron		WBbt:0006759
neuron of the amphid sensillum	amphid neuron	amphid sensory neuron	WBbt:0005394

1 Genes

WormBase Gene ID	Gene Name	Sequence Name	Organism
WBGene00016313	set-4	C32D5.5	Caenorhabditis elegans

1 Life Stages

Remark	Definition	Other Name	Public Name	Primary Identifier
	The whole period of embryogenesis in the nematode Caenorhabditis elegans, from the formation of an egg until hatching.		embryo Ce	WBls:0000003