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Expr9260
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In the head of both males and hermaphrodites, the expression of the Ptbb-4::YFP transcriptional reporter was observed in many amphid neurons, likely ADL, AFD, ASE, ASG, ASH, ASJ, ASK, ASI, AWA, AWB, and AWC as judged by co-labeling with lipophilic dyes and/or cellular morphology. Other head neurons expressing Ptbb-4::YFP include the cephalic CEP neurons, the outer labial quadrant OLQ neurons, and the mechanosensory FLP neurons. In the midbody and tail, Ptbb-4::YFP expression was found in the PDE postdeirid neurons, PQR (a sensory neuron whose cilium is exposed to the pseudocoelomic fluid), and the phasmid neurons PHA and PHB. Ptbb-4::YFP was also expressed in all 18 ray neurons in the male, consistent with a previous report (Portman and Emmons 2004). Because of abundant ray neuron expression, Ptbb-4::YFP expression in male phasmid and hook neurons could not be reliably confirmed, although expression in these cells is not unlikely. We also observed Ptbb-4::YFP expression in the hermaphrodite HSN motor neurons, the vulval muscles, and several unidentified nonsensory neurons. Expression was also occasionally apparent in the amphid sheath (AMsh) and socket (AMso) glial cells. |
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Expr9261
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In the head, a Ptba-9::YFP transcriptional reporter showed expression in amphid neurons, likely to be ADF, AFD, ASE, ASI, AWA, and AWC, in addition to the OLQ and CEP neurons, the anterior and posterior deirid neurons ADE and PDE, the RIG interneuron, and several additional unidentified nonsensory neurons. In the posterior of both sexes, this reporter was expressed in two tail interneurons, likely DVA, DVB, and/or DVC, as well as the sensory neuron PQR. In the male tail, Ptba-9::mCherry was expressed in the hook HOA and the ray RnA neurons as judged by morphol- ogy, position, and lack of overlap with the RnB marker Ppkd-2::GFP. In the head, a Ptba-9::YFP transcriptional reporter showed expression in amphid neurons, likely to be ADF, AFD, ASE, ASI, AWA, and AWC, in addition to the OLQ and CEP neurons, the anterior and posterior deirid neurons ADE and PDE, the RIG interneuron, and several additional unidentified nonsensory neurons. In the posterior of both sexes, this reporter was expressed in two tail interneurons, likely DVA, DVB, and/or DVC, as well as the sensory neuron PQR. In the male tail, Ptba-9::mCherry was expressed in the hook HOA and the ray RnA neurons as judged by morphology, position, and lack of overlap with the RnB marker Ppkd-2::GFP. |
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Expr14473
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The ttll-11a promoter drove GFP expression in a distinct and non-overlapping set of ciliated sensory neurons in the head, including the IL1s, outer labial quadrant neurons (OLQs), cephalic neurons (CEPs), and amphids, but not in the extracellular vesicle-releasing neurons EVNs. In the male tail, expression was seen in the hook A type neuron (HOA), ray type A neurons (RnAs), and phasmids but not the HOB and RnB neurons. Expression was also seen in phasmid neurons in the hermaphrodite tail. |
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