WormMine: ExpressionPattern Expr1078

• Pattern: Expressed in blastula embryos, not detailed description on the expression in later stages.
• Primary Identifier: Expr1078
• Remark: This fusion is functional, as it can rescue the embryonic lethality of a pie-1(0) mutant. In all 14 lines examined, GFP fluorescence in the adult germline and in embryos was observed in a pattern identical to that reported for PIE-1 using immunolocalization.
• Subcellular Localization: In embryos, PIE-1:GFP was found predominantly in the cytoplasm and nuclei of germline blastomeres. In the cytoplasm, PIE-1:GFP was present both diffusely throughout the cytosol and at higher concentration on P granules. PIE-1:GFP also appeared to associate with discrete foci in nuclei. The identity of these foci is not known. In oocytes and newly fertilized embryos, PIE-1:GFP was present uniformly throughout the cytoplasm. In the late 1-cell stage after the pronuclei have formed, PIE-1:GFP levels began to decrease in the anterior and increase in the posterior. By pronuclear meeting, PIE-1:GFP was found predominantly in the posterior. During mitosis, PIE-1:GFP also accumulated on both centrosomes with higher levels on the posterior centrosome. As a result of this asymmetric enrichment, most of the PIE-1:GFP was inherited by the posterior blastomere P1 during the first cleavage. In P1, P2, and P3, PIE-1:GFP distribution followed a sequence similar to that observed in the zygote, with the exception that PIE-1:GFP became increasingly more nuclear during each interphase. Before each cell division, PIE-1:GFP in the cytoplasm decreased on the side of the cell destined for the next somatic blastomere. At the start of mitosis, PIE-1:GFP disappeared from the nucleoplasm and became associated with centrosomes at both ends of the newly formed spindle. As mitosis progressed, PIE-1:GFP levels in the cytoplasm continued to decrease on the somatic side of the cell; concomitantly PIE-1:GFP levels decreased on the centrosome destined for the somatic daughter and increased on the centrosome destined for the germline daughter. After cytokinesis, most PIE-1:GFP was found in the germline daughter with only low levels left in the somatic daughter (e.g., EMS in the 4-cell stage). PIE-1:GFP fluorescence diminished progressively in that cell and was not detected in its progeny.

4 Anatomy Terms

Definition	Name	Synonym	Primary Identifier
Single cell zygote	P0	lineage name: Z	WBbt:0004422
embryonic founder cell	Psub3	lineage name: P0.ppp	WBbt:0006875
embryonic founder cell	Psub1	lineage name: P0.p	WBbt:0006874
embryonic founder cell	Psub2	lineage name: P0.pp	WBbt:0006873

1 Genes

WormBase Gene ID	Gene Name	Sequence Name	Organism
WBGene00004027	pie-1	Y49E10.14	Caenorhabditis elegans

1 Life Stages

Remark	Definition	Other Name	Public Name	Primary Identifier
	The C. elegans life stage spanning 0-100min after first cleavage at 20 Centigrade. Proliferate from 1 cell to 28 cells. From first cleavage until the start of gastrulation.		blastula embryo Ce	WBls:0000005