WormMine: ExpressionPattern Expr3651

• Pattern: The GFP::MLS-2 fusion construct and antibody staining showed identical expression patterns. Expression of MLS-2 was first detectable in one or two cells in embryos at the 50-cell stage and is localized in the nucleus. MLS-2 continued to be expressed in proliferating cells that are primarily located at the anterior of the embryo and are presumably derived from the AB lineage. During morphogenesis, this expression became restricted to a small subset of head neuronal precursors. Expression persisted in six head neurons during postembryonic development. GFP::MLS-2 expression was also observed in unidentified cells near the vulva at the L2 and L3 stages. To characterize the M lineage expression pattern of mls-2, double-labeling experiments were performed using anti-MLS-2 antibodies and the M lineage-specific hlh-8::gfp or hlh-8::lacZ markers. mls-2 expression in the M lineage was first detectable in the M mesoblast, and was retained during the first three rounds of cell divisions, such that mls-2 expression was still detectable in eight M descendants (designated 8-M stage, n>200). However, after one more round of cell division (at the 16-M stage), no MLS-2 signal was detected either by anti-MLS-2 antibodies or by the gfp::mls-2 fusion construct. Although it is possible that a low level of MLS-2 protein is present after the 8-M stage, the loss of MLS-2 signal at the 16-M stage appears to be due to the instability of the MLS-2 protein, because the mls-2 promoter is still active in M lineage descendants after the fourth round of cell division, as detected by a transcriptional mls-2p::gfp::mls-2 3' UTR construct. Neither the mls-2 promoter activity nor the MLS-2 protein was detected in the SM lineage or the differentiated BWMs and CCs. Thus mls-2 is expressed in the proliferating cells of the early M lineage.
• Primary Identifier: Expr3651
• Subcellular Localization: Localized in the nucleus.

16 Anatomy Terms

Definition	Name	Synonym	Primary Identifier
neuron with its cell body situated in the head, excluding the pharynx.	head neuron		WBbt:0006751
Postembryonic mesoblast of pedigree MSapaapp.	M cell	lineage name: MSapaapp	WBbt:0004489
post-embryonic cell of pedigree M.dla	M.dla		WBbt:0007162
post-embryonic cell of pedigree M.dl	M.dl		WBbt:0007161
post-embryonic cell of pedigree M.d	M.d		WBbt:0007160
post-embryonic cell of pedigree M.dr	M.dr		WBbt:0007169
post-embryonic cell of pedigree M.dlp	M.dlp		WBbt:0007165
post-embryonic cell of pedigree M.drp	M.drp		WBbt:0007173
post-embryonic cell of pedigree M.dra	M.dra		WBbt:0007170
post-embryonic cell of pedigree M.vla	M.vla		WBbt:0007179
post-embryonic cell of pedigree M.v	M.v		WBbt:0007177
post-embryonic cell of pedigree M.vlp	M.vlp		WBbt:0007182
post-embryonic cell of pedigree M.vra	M.vra		WBbt:0007192
post-embryonic cell of pedigree M.vrp	M.vrp		WBbt:0007195
post-embryonic cell of pedigree M.vl	M.vl		WBbt:0007178
post-embryonic cell of pedigree M.vr	M.vr		WBbt:0007191

1 Genes

WormBase Gene ID	Gene Name	Sequence Name	Organism
WBGene00003377	mls-2	C39E6.4	Caenorhabditis elegans

0 Life Stages