WormMine: ExpressionPattern Expr10148

• Pattern: In C. elegans L1 larvae, VBH-1 is localized mainly to granules associated to the nuclear envelope of germline precursor cells Z2 and Z3. A weak signal was also detected throughout the entire animal at L1 larval stage that we think might be either somatic cells expression or unspecific binding of the antibody. In C. elegans, Z2 and Z3 germ cells, as well as the somatic gonad precursors Z1 and Z4, start proliferating at mid-L1 stage. During L2 stage, the resultant germ cell population showed VBH-1 in perinuclear granules. By the end of the L2 stage, the nascent hermaphroditic gonad, including the population of germ cells, divides into an anterior and a posterior population that will form each gonad arm. In L3 larvae, both germ cell populations keep proliferating and extend away from the center longitudinally forming two long narrow groups of cells along the anterior-posterior axis of the nematode. VBH-1 specifically localized around the nuclei of these cells. The final larval L4 stage is characterized by a significant increase in the germline cell population. In the L4 stage, spermatogenesis takes place in the hermaphrodite and male gonads. Germline cells remains as the main site where VBH-1 signal is detected in granules with a perinuclear arrangement.
• Primary Identifier: Expr10148

3 Anatomy Terms

Definition	Name	Synonym	Primary Identifier
Germ line precursor cell	Z2	lineage name: P4.p	WBbt:0004576
Germ line precursor cell	Z3	lineage name: P4.a	WBbt:0004575
a cell, of either sex, directly concerned in the production of a new organism.	germ cell		WBbt:0006796

1 Genes

WormBase Gene ID	Gene Name	Sequence Name	Organism
WBGene00006888	vbh-1	Y54E10A.9	Caenorhabditis elegans

5 Life Stages

Remark	Definition	Other Name	Public Name	Primary Identifier
	The stage that begins when a C.elegans individual is fully-developed and has reached maturity.		adult Ce	WBls:0000041
	The third stage larva. At 25 Centigrade, it ranges 32.5-40 hours after fertilization, 18.5-26 hours after hatch.		L3 larva Ce	WBls:0000035
	The second stage larva. At 25 Centigrade, it ranges 25.5-32.5 hours after fertilization, 11.5-18.5 hours after hatch.		L2 larva Ce	WBls:0000027
	The fourth stage larva. At 25 Centigrade, it ranges 40-49.5 hours after fertilization, 26-35.5 hours after hatch.		L4 larva Ce	WBls:0000038
	The first stage larva. At 25 Centigrade, it ranges 14-25.5 hours after fertilization, 0-11.5 hours after hatch.		L1 larva Ce	WBls:0000024