| Pattern | SMU-2::GFP is a nuclear protein. SMU-2::GFP was observed at all stages of development including early embryogenesis and in oocytes. To show that SMU-2 is ubiquitously expressed, embryos and larvae carrying the integrated smu-2::gfp array were costained with the DNA stain DAPI and SMU-2::GFP was observed in essentially every cell that was stained by DAPI. Unlike most transgenes in C. elegans, the smu-2::gfp transgene was expressed in the germ line. To confirm this result with another SMU-2-expressing transgene, a rescuing transgene that placed a HA tag at the very end of the protein were constructed. This construct also led to nuclear expression in all cells, including the germ line. | Primary Identifier | Expr3427 |
| Subcellular Localization | SMU-2::GFP was occasionally observed localized to a subnuclear compartment that probably is the nucleolus because of its morphology and its increased size in ncl-1 mutants, which have enlarged nucleoli. However, nucleolar localization was dependent on the health of the animal. Animals suffering from prolonged exposure to sodium azide showed a dramatic increase in nucleolar localization. Nucleolar localization of SMU-2::HA was rarely observed. SMU-2 probably is not normally localized to the nucleolus. No other consistent pattern of subnuclear localization was observed. |
| Definition | Name | Synonym | Primary Identifier |
|---|---|---|---|
| a cellular object that consists of subcellular components, expresses genes or functions. | Cell | Cell type | WBbt:0004017 |
| WormBase Gene ID | Gene Name | Sequence Name | Organism |
|---|---|---|---|
| WBGene00004896 | smu-2 | Y49F6B.4 | Caenorhabditis elegans |
