WormMine: ExpressionPattern Expr1867

• Pattern: A transgene that includes only the sid-1 promoter region fused to GFP (sid-1 pro::GFP), is expressed in late embryos and continues to be detected in nearly all nonneuronal cell types through adulthood. The highest levels of GFP expression in the adult are in the cells and tissues in direct contact with the environment. These environment-exposed cells also show expression of a transgene construct in which GFP was fused to the COOH-terminus of full-length SID-1 (sid-1::C-GFP). This construct was capable of rescuing sid-1(qt2) animals, suggesting that localization of this reporter is representative of SID-1 localization. Expression of SID-1::C-GFP was only seen in these environment exposed cells, perhaps because of the significantly lower expression of the translational reporter relative to the transcriptional reporter.
• Primary Identifier: Expr1867
• Subcellular Localization: In addition to cytoplasmic expression, significant enrichment of subcellular localization of SID-1::C-GFP was seen at the cell periphery.

1 Anatomy Terms

Definition	Name	Synonym	Primary Identifier
a cellular object that consists of subcellular components, expresses genes or functions.	Cell	Cell type	WBbt:0004017

1 Genes

WormBase Gene ID	Gene Name	Sequence Name	Organism
WBGene00004795	sid-1	C04F5.1	Caenorhabditis elegans

6 Life Stages

Remark	Definition	Other Name	Public Name	Primary Identifier
	The stage that begins when a C.elegans individual is fully-developed and has reached maturity.		adult Ce	WBls:0000041
	The third stage larva. At 25 Centigrade, it ranges 32.5-40 hours after fertilization, 18.5-26 hours after hatch.		L3 larva Ce	WBls:0000035
	The second stage larva. At 25 Centigrade, it ranges 25.5-32.5 hours after fertilization, 11.5-18.5 hours after hatch.		L2 larva Ce	WBls:0000027
	The fourth stage larva. At 25 Centigrade, it ranges 40-49.5 hours after fertilization, 26-35.5 hours after hatch.		L4 larva Ce	WBls:0000038
	The first stage larva. At 25 Centigrade, it ranges 14-25.5 hours after fertilization, 0-11.5 hours after hatch.		L1 larva Ce	WBls:0000024
	The C. elegans life stage spanning 620-800min(hatch) after first cleavage at 20 Centigrade. Cell number remains at ~560 cells, with some new cells generated and some cells go through programmed cell death. A stage after elongation is over. The last stage of embryogenesis. Also called pre-hatched embryo, late embryo or morphogenetic embryo.		fully-elongated embryo Ce	WBls:0000021