WormMine

WS295

Intermine data mining platform for C. elegans and related nematodes

Expression Pattern :

Pattern  No CEC-1 could be detected in zygotes and early embryos. The protein was first detected in embryos at about the 50- to 80-cell stage. At this stage, weak immunostaining appeared in all somatic nuclei. Again, the specificity of the antibodies was confirmed by preincubating the serum with the purified cec-1-antigen which abolished all observed signals. Later in development, nuclear staining becomes stronger and persists in all somatic cells of wild-type and transgenic embryos and larvae. In adult wild-type worms, CEC-1 is present in all somatic nuclei. The intensity of the signals varied with the size of the nuclei; the large polyploid nuclei of the hypodermis and intestine showed intense immunofluorescence, while the smaller diploid nuclei had weaker signals. Neither in wild-type nor in transgenic animals were detected any nuclear staining in the germ line cells, in the germ precursor cells Z2 and Z3, and in early blastomeres. Furthermore, the presence of CEC-1 in the cytoplasm of these cells was excluded by comparison with control animals treated with the preimmune serum. Primary Identifier  Expr1599
Subcellular Localization  CEC-1 staining in interphase nuclei was homogeneous and no speckles or foci could be detected, suggesting a uniform distribution of the protein. High-resolution imaging of the anti-CEC-1 antibody staining also revealed uniform staining of interphase nuclei. Interestingly, when the chromosomes started to condense during early mitosis, the protein appeared to dissociate from them, as shown in prometaphase nuclei. In fully condensed metaphase chromosomes, the protein was no longer associated with them.

1 Anatomy Terms

Definition Name Synonym Primary Identifier
a cellular object that consists of subcellular components, expresses genes or functions. Cell Cell type WBbt:0004017

1 Genes

WormBase Gene ID Gene Name Sequence Name Organism
WBGene00000414 cec-1 ZK1236.2 Caenorhabditis elegans

5 Life Stages

Remark Definition Other Name Public Name Primary Identifier
  The C. elegans life stage spanning 620-800min(hatch) after first cleavage at 20 Centigrade. Cell number remains at ~560 cells, with some new cells generated and some cells go through programmed cell death. A stage after elongation is over. The last stage of embryogenesis. Also called pre-hatched embryo, late embryo or morphogenetic embryo. fully-elongated embryo Ce WBls:0000021
  The C. elegans life stage spanning 350-620min after first cleavage at 20 Centigrade. Cell number remains at ~560 cells, with some new cells generated and some cells go through programmed cell death. The stage that embryo starts elongation until elongation is over. elongating embryo Ce WBls:0000015
  The C. elegans life stage spanning 290-350min after first cleavage at 20 Centigrade. Proliferate from 421 cells to 560 cells. The stage when embryo just finished gastrulation and is enclosing. enclosing embryo Ce WBls:0000013
  The C. elegans life stage spanning 100-290min after first cleavage at 20 Centigrade. Proliferate from 28 cells to 421 cells. Referring to the whole period of gastrulation. gastrulating embryo Ce WBls:0000010
  A developemental life stage of the nematode Caenorhabditis elegans that occurs from egg hatching until death. postembryonic Ce WBls:0000022