| Pattern | In general, the expression patterns found for the translational fusion constructs were similar to those reported above for the transcriptional fusions. Expression of NHX-4::GFP was observed in nearly all cells and cell types. Labeling was observed at the nerve ring and retrovesicular ganglia, as well as weak intestinal fluorescence, and signal in the hypodermal cells, in particular those associated with the intestinal tract and rectum. | Primary Identifier | Expr2244 |
| Subcellular Localization | NHX-4::GFP was targeted to the plasma membrane. NHX-4::GFP can clearly be seen to partition in a distinct pattern at the cell membrane of body wall muscle with the twisting characteristic of the rol-6 phenotype. This pattern appears to indicate some specific targeting of NHX-4::GFP to a microdomain in the membrane, perhaps where contact with other cells takes place. However, in most cells, the NHX-4::GFP protein is distributed evenly throughout the membrane, with the cell bodies of neurons clearly outlined, and fluorescence extending through the neuronal processes. When expressed in polarized epithelial cells, NHX-4::GFP appears to be targeted to the basolateral membrane. |
| Definition | Name | Synonym | Primary Identifier |
|---|---|---|---|
| a cellular object that consists of subcellular components, expresses genes or functions. | Cell | Cell type | WBbt:0004017 |
| WormBase Gene ID | Gene Name | Sequence Name | Organism |
|---|---|---|---|
| WBGene00003732 | nhx-4 | F14B8.1 | Caenorhabditis elegans |
