WormMine: ExpressionPattern Expr2822

• Pattern: When transgenic worms harboring the GFP-tagged rcq-5 gene were observed under a fluorescence microscope, GFP was localized in nuclei of late embryos at the 3-fold stage and in the nuclei of many somatic cells in larvae and adult worms. Although GFP appeared ubiquitous in the worms, its intensity was relatively strong in the intestine, which coincided with the intestinal GFP expression in late embryos. The main reason why GFP expression was not seen in early embryos and gonads is cosuppression mediated by multi-copy transgenes in the C. elegans germ-line. A 0.5 kb long 5 -upstream DNA was adequate to induce reporter gene expression with the same pattern.
• Primary Identifier: Expr2822
• Remark: Reporter gene fusion type not specified.
• Subcellular Localization: nuclei

1 Anatomy Terms

Definition	Name	Synonym	Primary Identifier
a cellular object that consists of subcellular components, expresses genes or functions.	Cell	Cell type	WBbt:0004017

1 Genes

WormBase Gene ID	Gene Name	Sequence Name	Organism
WBGene00004322	rcq-5	E03A3.2	Caenorhabditis elegans

6 Life Stages

Remark	Definition	Other Name	Public Name	Primary Identifier
	The stage that begins when a C.elegans individual is fully-developed and has reached maturity.		adult Ce	WBls:0000041
	The third stage larva. At 25 Centigrade, it ranges 32.5-40 hours after fertilization, 18.5-26 hours after hatch.		L3 larva Ce	WBls:0000035
	The second stage larva. At 25 Centigrade, it ranges 25.5-32.5 hours after fertilization, 11.5-18.5 hours after hatch.		L2 larva Ce	WBls:0000027
	The fourth stage larva. At 25 Centigrade, it ranges 40-49.5 hours after fertilization, 26-35.5 hours after hatch.		L4 larva Ce	WBls:0000038
	The first stage larva. At 25 Centigrade, it ranges 14-25.5 hours after fertilization, 0-11.5 hours after hatch.		L1 larva Ce	WBls:0000024
	The whole period of embryogenesis in the nematode Caenorhabditis elegans, from the formation of an egg until hatching.		embryo Ce	WBls:0000003