WormMine: ExpressionPattern Expr2832

• Pattern: Immunolocalization analysis with a polyclonal antibody generated against the carboxyl terminus of EGL-30 generally revealed the areas with highest expression levels. Using an HRP-conjugated secondary antibody and a FITC-conjugated secondary antibody, high expression was observed in axons of the nerve ring. The FITC-conjugated antibody also indicated expression in pharyngeal muscle. Both secondary antibodies revealed expression in sperm, and the FITC-conjugated antibody consistently showed expression in the anal sphincter muscle. Weak or occasional expression in other muscle-cell types, sperm, intestine, tonofilaments, and vulval cells indicates the possibility that EGL-30 might have an almost ubiquitous expression pattern.
• Primary Identifier: Expr2832
• Remark: Nonspecific antibody staining was observed in intestinal nuclei using both secondary antibodies and is most likely due to cross-reactivity with an unrelated protein. Western analysis supports this hypothesis since the band that migrates at the position where EGL-30 migrates on an SDS/PAGE gel is present at almost equivalent intensities in protein extracts prepared from egl-30(ad805) mutants compared to wild-type extracts, but the levels of this band increase significantly after heat-shock induction of an egl-30 transgene. When egl-30 was overexpressed from an integrated array (syIs36), an increase in the intensity of staining was observed in the nerve ring and in pharyngeal muscle, but not in intestinal nuclei.
• Subcellular Localization: Expressed in axons of the nerve ring.

1 Anatomy Terms

Definition	Name	Synonym	Primary Identifier
a cellular object that consists of subcellular components, expresses genes or functions.	Cell	Cell type	WBbt:0004017

1 Genes

WormBase Gene ID	Gene Name	Sequence Name	Organism
WBGene00001196	egl-30	M01D7.7	Caenorhabditis elegans

6 Life Stages

Remark	Definition	Other Name	Public Name	Primary Identifier
	The stage that begins when a C.elegans individual is fully-developed and has reached maturity.		adult Ce	WBls:0000041
	The third stage larva. At 25 Centigrade, it ranges 32.5-40 hours after fertilization, 18.5-26 hours after hatch.		L3 larva Ce	WBls:0000035
	The second stage larva. At 25 Centigrade, it ranges 25.5-32.5 hours after fertilization, 11.5-18.5 hours after hatch.		L2 larva Ce	WBls:0000027
	The fourth stage larva. At 25 Centigrade, it ranges 40-49.5 hours after fertilization, 26-35.5 hours after hatch.		L4 larva Ce	WBls:0000038
	The first stage larva. At 25 Centigrade, it ranges 14-25.5 hours after fertilization, 0-11.5 hours after hatch.		L1 larva Ce	WBls:0000024
	The whole period of embryogenesis in the nematode Caenorhabditis elegans, from the formation of an egg until hatching.		embryo Ce	WBls:0000003