| Pattern | The nlg-1 promoter expressed GFP in cholinergic motor neurons but not in GABAergic neurons. | Primary Identifier | Expr10537 |
| Reporter Gene | The transcriptional reporter for nlg-1 used 6.5kb of 5' flanking sequence. A 2.4kb myo-3 myosin promoter was used for expression in body muscles, a 3.2kb unc-17 VAChT promoter was used for expression in cholinergic neurons, and a 2.6kb unc-129 promoter was used for expression in DA and DB motor neurons. | Subcellular Localization | When expressed in cholinergic DA and DB motor neurons, NLG-1::GFP exhibited a punctate distribution in dorsal cord axons but was diffuse in ventral cord dendrites. Dorsal cord NLG-1 puncta co-localized with a synaptic vesicle (SV) marker (mCherry-tagged UNC-57 endophilin). NRX-1::GFP expressed in body muscles was also punctate in the nerve cords and these NRX-1 puncta were often closely apposed to pre-synaptic UNC-57 puncta. |
| Definition | Name | Synonym | Primary Identifier |
|---|---|---|---|
| neuron that uses vesicular acetylcholine as a neurotransmitter. | cholinergic neuron | ACh neuron | WBbt:0006840 |
| WormBase Gene ID | Gene Name | Sequence Name | Organism |
|---|---|---|---|
| WBGene00006412 | nlg-1 | C40C9.5 | Caenorhabditis elegans |
