WormMine: AnatomyTerm WBbt:0006790

• Definition: monoucleate interfacial cell between the uterus and vulva (uv1-uv3), four sets total (L/R, A/P)
• Name: uterine-vulval cell
• Primary Identifier: WBbt:0006790
• Synonym: uv cell

3 Children

Definition	Name	Synonym	Primary Identifier
uterine-vulval cell proximal to the vulva.	uv1		WBbt:0006791
uterine-vulva cell, distal (of three ut cells) to the vulva.	uv3		WBbt:0006793
uterine-vulval cell, medial position of three ut cells.	uv2		WBbt:0006792

1 Expression Clusters

Regulated By Treatment	Description	Algorithm	Primary Identifier
	Single-cell RNA-Seq cell group 48 expressed in: Uterine-vulval cells.	CellRanger, DecontX, Monocle3, Louvain algorithm.	WBPaper00065623:48

3 Expression Patterns

• Primary Identifier: Expr15194
• Pattern: We consistently observed aat-1::mCherry fluorescence in several pairs of head neurons; pharynx; reproductive system -including vulval muscles, uterine muscles, and uterine-vulva cells; excretory cells; and weak transgene expression in the intestine of adult animals. Reporter fusions controlled by the aat-1 and nkat-1 promoters showed overlapping expression patterns in RMDV and RIM neurons

• Primary Identifier: Expr12514
• Pattern: IFA-2::GFP protein localizes to the epidermal fibrous organelles (hemidesmosomes), the uterine seam and touch neurons. Uterine expression includes both utse and uv cells. The seam expression appears at the L4-adult molt and probably correlates with expression of IFA-2 in both the utse and the seam cells as they terminally differentiate.

• Primary Identifier: Expr16522
• Pattern: Interestingly, when imaging the FOLR-1::mNeonGreen-strain at the L4 larval stage, we found that FOLR-1 can only be detected in a few cells close to the vulva. Notably, the single-cell resolution atlas of gene activity of young adult C. elegans (WormSeq application) revealed that folr-1 mRNA is expressed at least in uterine-vulval cells, dorsal uterine cell and the RIS neuron. By comparing the expression profile of FOLR-1::mNeonGreen with the structural anatomy of the C. elegans reproductive system, we found that FOLR-1 can be detected at least in uterine-vulval cells. There are three types of uterine-vulval cells (uv-1-3), which form a multilayered set of flaps that bind the ventral uterus to the dorsalmost ring of the vulva. The expression in uterine-vulval cells becomes visible at the late L4 stage when the vulval lumen begins to narrow. The FOLR-1::mNeonGreen signal reaches its strongest level when the vulval lumen closes at the transition from the late L4 stage to the young adult stage. On the contrary to the late L4 stage, the FOLR-1::mNeonGreen signal cannot be detected at mid-L4 stage. Furthermore, the FOLR-1::mNeonGreen signal is gone in most of the gravid day 1 adults, demonstrating that FOLR-1 is expressed in a developmental stage-specific manner. Together, these assays (single-cell RNA-seq and imaging of the FOLR-1::mNeonGreen-expressing strain) demonstrate that FOLR-1 can only be detected in a few cell types. However, it is noteworthy that FOLR-1 can also be expressed in other tissues (such as the RIS neuron), but the sensitivity of FOLR-1::mNeonGreen may not be sufficient to detect it at low levels.

0 Life Stages

3 Parents

Definition	Name	Synonym	Primary Identifier
The organ in which the eggs are developed and protected until laid.	uterus		WBbt:0006760
cell of the epithelia of uterus.	uterine epithelial cell		WBbt:0008435
somatic (not germline) cell of the hermaphrodite gonad.	hermaphrodite somatic gonadal cell		WBbt:0007815