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Expr1455
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Almost every transgenic animal shows strong DAF-3/GFP expression in many, but not all, head neurons, the ventral nerve cord (both cell bodies and processes), the intestinal cells, especially the membrane adjacent to the intestinal lumen, and tail hypodermal cells and neurons. Weak expression in the pharynx, hypodermal V blast cells, P blast cells and hyp7 hypodermal cells is observed in about half of the transgenic animals. Expression in the tail hypodermal cells hyp 9, hyp 10, and hyp 11 is clearly seen in nearly every animal. This apparent difference between tail hypodermal expression and main body expression may be a consequence of the anatomy of the animal. The main body hypodermis is underlain by bright GFP in the intestine and ventral nerve cord, so weak expression in the hypodermis is hard to see against this background. Expression is rarely detected in dorsal body wall muscle. DAF-3/GFP is expressed in the distal tip cells and in their precursors, Z1.a and Z4.p, throughout development. DAF-3/GFP is also expressed strongly in unidentified vulval cells in adults. In wild-type embryos of 200 to 400 cells, DAF-3/GFP is expressed uniformly thoughout the embryo. |
In wild type, DAF-3/GFP is primarily, although not exclusively, cytoplasmic. DAF-3/GFP subcellular distribution was examined in head neurons in the vicinity of ASI (the cell that produces the DAF-7 signal), as well as in intestinal cells. DAF-3/GFP was predominantly cytoplasmic in all animals. However, in all animals, dim GFP fluorescence was seen in the nucleus of some of the cells with bright cytoplasmic fluorescence, and in 25% of the animals, equivalent DAF-3/GFP levels in the nucleus and cytoplasm was observed in one or more cells. |
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Expression pattern at adult stage was not described. |
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Expr1410
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Expression in Z1 and Z4, the somatic gonad precursors, starts a few hours after these are born. Up to the formation of the somatic gonad primordium in late L2, LIN-26 protein was detected in all cells of the somatic gonad, except in the distal tip cells or dtcs (Z1.aa and Z4.pp). Staining was strongest in Z1 and Z4 and became weaker after each cell division. However, after Z1.a and Z4.p divided, LIN-26 was initially not detected in any of their daughters, but resynthesis occurred in Z1.ap and Z4.pa. During the L3 stage, expression was only detected in the anchor cell (AC) until the Pn.pxx cells were generated. After the last division round in L4 larvae, all uterine nuclei and two spermathecal-uterine junction nuclei expressed LIN-26 at very low levels. |
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