1 Genes
| WormBase Gene ID | Gene Name | Sequence Name | Organism |
|---|---|---|---|
| WBGene00006525 | tax-2 | F36F2.5 | Caenorhabditis elegans |
| Primary Identifier | Expr11197 | Reporter Gene | [srbc-66p::tax-2::gfp] translational fusion. srbc-66p::tax-2::gfp was generated by fusing tax-2 cDNA in frame to gfp in an expression vector containing 2.1 kb of srbc-66 upstream regulatory sequences (Kim et al., 2009). |
| Subcellular Localization | TAX-2::GFP expressed under an AWB-specific promoter (str-1p::tax-2::gfp) was localized to a proximal region of each of the AWB neuron ciliary branches and excluded from the distal regions. The region of TAX-2 localization was distal to the basal body/transition fiber zone marked by CHE-13::TagRFP IFT protein accumulation, as well as the transition zone marked by localization of an MKSR-2::TagRFP fusion protein. The region of TAX-2::GFP localization was instead reminiscent of the Inversin compartment observed in several cilia types in vertebrates. As in mammalian cells, the Inversin homolog NPHP-2 is localized to the middle segment in C. elegans sensory cilia and shown to play a role in placement of the transition zone. The authors found that localization of TAX-2::GFP in AWB cilia overlapped almost fully with that of NPHP-2::mCherry. TAX-2::GFP expression was not observed in AWB axons. When expressed under an ASK-specific promoter (srbc-66), TAX-2 was localized to the middle segment, distal to the basal body/transition fiber region. Therefore TAX-2 is localized to the middle segments of both AWB and ASK cilia. |
| WormBase Gene ID | Gene Name | Sequence Name | Organism |
|---|---|---|---|
| WBGene00006525 | tax-2 | F36F2.5 | Caenorhabditis elegans |
