WormMine: ExpressionPattern Expr1287

• Pattern: Blastula embryo.
• Primary Identifier: Expr1287
• Remark: No detailed description on cellular localization at later stages.
• Subcellular Localization: The antibodies stained the cell periphery of early blastomeres as well as the nuclear envelope. Nuclear envelope staining however, appears to be non-specific. The staining in 1-cell embryos is weak and uniform just after the completion of meiosis, but increases in intensity and becomes concentrated at the anterior periphery during pronuclear migration. The peripheral PKC-3 staining becomes restricted to about 50% embryo length during the pronuclear meeting and pronuclear fusion stage (n=23). By early anaphase, the staining extends posteriorly beyond the midline of the zygote and covers about 60% of the total length of embryos (n=29). In 2- and 4-cell stages, staining is uniform at the periphery of the AB cell, its daughters and the EMS cell, but peripheral staining in P1 and P2 is restricted to the boundaries with other blastomeres.

0 Anatomy Terms

1 Genes

WormBase Gene ID	Gene Name	Sequence Name	Organism
WBGene00004034	pkc-3	F09E5.1	Caenorhabditis elegans

4 Life Stages

Remark	Definition	Other Name	Public Name	Primary Identifier
	The C. elegans life stage spanning 0-100min after first cleavage at 20 Centigrade. Proliferate from 1 cell to 28 cells. From first cleavage until the start of gastrulation.		blastula embryo Ce	WBls:0000005
	The C. elegans life stage spanning 0-20min after first cleavage at 20 Centigrade. Contains 2 cells.		2-cell embryo Ce	WBls:0000007
	Also called zygote. -50min till 0min of first cleavage at 20 Centigrade. Contains 1 cell.		1-cell embryo Ce	WBls:0000006
	The C. elegans life stage spanning 20-40min after first cleavage at 20 Centigrade. Contains 4 cells.		4-cell embryo Ce	WBls:0000008