1 Genes
| WormBase Gene ID | Gene Name | Sequence Name | Organism |
|---|---|---|---|
| WBGene00006575 | tir-1 | F13B10.1 | Caenorhabditis elegans |
| Primary Identifier | Expr3965 | Remark | A similar punctate localization pattern of TIR-1 was present in animals bearing a rescuing odr-3::tir-1::DsRed transgene. Both TIR-1::GFP and TIF-1::RFP can restore wild-type tir-1 function. With the qualification that they were obtained with overexpressed or tagged proteins, these results suggest that TIR-1 protein is localized to a subcompartment in AWC axons. To ask whether TIR-1 is localized to presynaptic or post-synaptic sites in the AWC axon, the odr-3::tir-1::DsRed transgene was coexpressed with str-2::synaptobrevin (snb-1)::GFP, a GFP-tagged synaptic vesicle protein that serves as a presynaptic marker, or odr-3::lin-10::YFP, a YFP-tagged PDZ-domain protein that localizes to post-synaptic densities in C. elegans neurons. TIR-1::DsRed was strikingly colocalized with LIN-10::YFP, but was usually adjacent to SNB-1::GFP rather than overlapping with it, despite the greater overall expression of SNB-1::GFP. These results suggest that TIR-1 may be localized to post-synaptic regions of AWC. |
| Subcellular Localization | TIR-1::GFP was localized in a punctate pattern along the AWC axon, and mostly excluded from the cell body and dendrites, like TIR-1 detected by the antiserum. |
| WormBase Gene ID | Gene Name | Sequence Name | Organism |
|---|---|---|---|
| WBGene00006575 | tir-1 | F13B10.1 | Caenorhabditis elegans |
