WormMine: AnatomyTerm WBbt:0004102

• Definition: Interneuron, associated with PLM
• Name: PLNL
• Primary Identifier: WBbt:0004102
• Synonym: lineage name: TL.pppap

1 Children

Definition	Name	Synonym	Primary Identifier
nucleus of pedigree TL.pppap	TL.pppap nucleus		WBbt:0002620

1 Expression Clusters

Regulated By Treatment	Description	Algorithm	Primary Identifier
	Single-cell RNA-Seq cell group 28_0 expressed in neuron.	scVI 0.6.0	WBPaper00065841:28_0

63 Expression Patterns

• Primary Identifier: Expr4841
• Pattern: A-class motor neuron: expressed in embryo; not expressed in larva. Neuronal expression include: All ventral cord motor neurons, head and tail neurons, PLN. Also expressed in other cells: Pharyngeal muscle, vulval muscle, anal depressor, hypodermis. Pan-neuronal: enriched in embryo (1.8); not expressed in larva.

• Primary Identifier: Expr15558

• Primary Identifier: Expr15567

• Primary Identifier: Expr15571

• Primary Identifier: Expr15572

• Primary Identifier: Expr15573

• Primary Identifier: Expr15579

• Primary Identifier: Expr2937
• Pattern: Both ahr-1:GFP reporters are expressed during embryonic and larval development. Expression is first detected in two cells 260 min after the first cleavage. By midembryogenesis (pre-comma stage), 14 cells express the pJ360 ahr-1:GFP fusion gene. At the 2-fold stage of embryogenesis, two cells express ahr-1:GFP in the tail, and the remaining fluorescing cells are in the forming head. During the first larval stage. ahr-1:GFP is expressed in 28 neurons, several blast cells, and two phasmid socket cells. The neurons that express ahr-1:GFP include ALNR/ALNL, AQR/PQR, AVM/PVM, BDUR/BDUL, PLMR/PLML, PLNR/PLNL, PHCL/PHCR, PVWL/PVWR, RMEL/RMER, SDQR/SDQL, and URXR/URXL. The T.pa, T.ppa, and T.ppp blast cells in the tail express ahr-1:GFP, as do all of their descendents, including the PHso1 and PHso2 phasmid socket cells. ahr-1:GFP is also expressed in the MI and I3 neurons in the pharynx and the G2 and W blast cells. Four additional cells in the head express ahr-1:GFP, tentatively identified as the ASK and RIP neurons.
• Subcellular Localization: The pJ360 construct includes the entire ahr-1 genomic sequence, and transgenic animals express this fusion protein in a subset of neuronal nuclei. The pHT102 transgene lacks most of the ahr-1 coding sequence and labels axons as well as nuclei.

• Primary Identifier: Expr15586

• Primary Identifier: Expr15651

• Primary Identifier: Expr15652

• Primary Identifier: Expr15589

• Primary Identifier: Expr13158

• Primary Identifier: Expr15591

• Primary Identifier: Expr13164
• Pattern: For lgc-38, all expressing cells shown are observed with the 3.5 kb reporter fusion, except for OLL, which only expresses the 3.9 kb fusion; URA expresses both.

• Primary Identifier: Expr15598

• Primary Identifier: Expr15604

• Primary Identifier: Expr14590
• Pattern: Embryonic expression of exc-7 was first observed at the bean stage. By reverse lineaging with use of SIMI-Biocell software, we confirm the identity of one of the expressing cells at this stage as the excretory canal cell. In L1 animals, broad expression in the head, ventral nerve cord (VNC), and tail was observed. In young adults, expression is notably observed in vulva cells. In the nervous system specifically, expression is observed in many neurons throughout the body, but unlike Drosophila Elav, exc-7::gfp it is not panneuronally expressed. We confirmed previously reported expression in cholinergic VNC MNs, but absence of GABAergic VNC MNs, consistent with previous reports (Fujita et al., 1999; Loria et al., 2003) and consistent with exc-7 functioning in cholinergic, but not GABAergic neurons to control alternative splicing (Norris et al., 2014). exc-7::gfp is also expressed in some non-neuronal cell types, including muscle and hypodermis, but not in the gut. A previous report showed that exc-7 is only transiently and weakly expressed in the excretory cell, which, based on exc-7's excretory mutant phenotype, has puzzled researchers (Fujita et al., 2003). We find that the gfp tagged exc-7 locus is strongly and continuously expressed in the excretory canal cell.

• Primary Identifier: Expr15608

• Primary Identifier: Expr15611

• Primary Identifier: Expr946
• Remark: To compare the transcription pattern of the daf-1 promoter with the whole gene, the gfp cDNA was fused to the terminus of the daf-1 gene in a plasmid that included 2.6 kb of upstream sequence. Despite the fact that this transgene rescued the daf-1 Daf-c mutant phenotype, GFP fluorescence was not detected in rescued animals. Hence, these observations were limited to the daf-1 promoter fusion, which may not represent the expression pattern of the whole daf-1 gene if enhancer elements are present in introns or in 3' sequences.
• Pattern: GFP expression was observed in the head and the developing ventral nerve cord beginning in mid-stage embryos and continuing into adulthood. In the head, GFP was detected in more than twenty neurons in the anterior, lateral, ventral and retrovesicular ganglia. Fluorescent processes terminating at the tip of the head suggest that daf-1 is expressed in sensory neurons and in support cells in the amphids and inner labial sensilla. In the midbody, GFP was expressed in the ALM mechanosensory neurons and the PVT neuron, as well as one additional neuron pair. In the lumbar ganglia of the tail, five cells expressing GFP included phasmid neurons and PLN and PLM mechanosensory neurons. The daf-1 promoter also conferred gfp expression in nonneuronal cells, including a membranous sheath surrounding the distal end of the intestine and in the distal tip cell (DTC) of the gonad. In some lines, GFP was sometimes detected in the muscles of L4 and adult animals. In L1 larvae, daf-1 promoters is active in neurons in the head, as well as in the ventral cord and tail. The promoter continues to express GFP in dauer larvae from starved plates.

• Primary Identifier: Expr733
• Remark: This information was extracted from published material (Archana Sharma-Oates, Andrew Mounsey and Ian A. Hope).
• Pattern: Staining is seen in a set of 47 nuclei in fixed newly hatched first larval stage (L1). All stained cells are neurons. Hermaphrodite express in cells. RIH, RIR, PVR, IL2L/R, URYVL/R, RIPL/R, AIZL/R, FLPL/R, ADAL/R, RMGL/R, BPUL/R, PLML/R, ALML/R, ALNL/R, HSNL/R, URBL/R, NSML/R, URADL/R, IL2DL/R, I2L/R, IL2VL/R, URAVL/R, URXL/R, AIML/R, URYDL/R, PQR, PVM, SDQL/R, PVDL/R, PHCL/R, PLNL/R. Male cells express as in hermaphrodite except for HSNL/R which die, and show expression in CEMDL/R, CEMVL/R which die in hermaphrodites. Expression pattern is first determined in the Q lineage. Once expression has been initiated in a cell, it is maintained by that cell and all of its descendants in all cases except for SDQ.

• Primary Identifier: Expr14053
• Remark: Other Strain: OH14242
• Pattern: ASI, ASJ, aprox 3 more pairs in head, (PLM), (PLN), (ALN), sometimes AVM & PVM, sometimes PVT, sometimes pharynx, anal depressor muscle.

• Primary Identifier: Expr14117
• Pattern: Several head sensory neurons (ASI, ASH, ASJ), one neuron pair in ventral ganglion, AVG, ALN, PLN, pharynx

• Primary Identifier: Expr8675
• Remark: Picture: N.A.
• Pattern: Expression in the alimentary canal: Strong and consistent expression in pm5, MC. Weak or rare expression in pharyngeal epithelium, pm1, pm2, pm3, pm4 pm6, pm7, pm8, g1, g2, rectal gland cells. Expression in the nervous system: ADE, AIY, ALM, ALN, AVA, AVK, AVM, BDU, CAN, DAn, DVA, DVB, DVC, FLP, HSN, LUA, PLM, PLN, PVC, PVM, PVP, PVQ, PVT, PVW, RID, RIS, SDQ, URB, MC. Expression in the reproductive system: In adult stage, expressed in HSN. Faint hypodermal expression of inx-7 is seen around two-fold stage and becomes stronger by threefold stage.

• Primary Identifier: Expr320
• Remark: Neuronal gene expression pattern from collation by Shawn Lockery of neuron-specific promotors posted 20/04/98 (http://chinook.uoregon.edu). Since cell AS is listed under 'body' in the pattern, it presumably means the AS.1-11 ventral cord neurons rather than the AS amphid neurons.[sdm-curator]
• Pattern: head: IL2 URA URB SAA SAB SIA SIB SMB SMD RMD AIY, sev more; phar: M1 M2 M5 I1f I6f, others; body: VA VB VC DA DB AS SDQ HSNf; tail: ALN PLN others [J. Duerr (personal communication to Shawn Lockery)], antibody

• Primary Identifier: Expr8694
• Remark: Picture: Fig 3.
• Pattern: Expression in the alimentary canal: Strong and consistent expression in M5, I1, I3, I6, NSM. Weak or rare expression in posterior arcades. Expression in the nervous system: Phsh, ADA, ADE, ADL, AIN, AIY, ALM, AUA, AVA, AVD, AVH, AVJ, AVK, AVM, AWB, BDU, CAN, CEP, DAn, DBn, DDn, DVB, DVC, FLP, HSN, IL1, IL2, LUA, OLL, PDA, PDB, PDE, PHA, PHB, PHC, PLM, PLN, PVC, PVD, PVM, PVN, PVP, PVQ, PVR, PVT, PVW, RIB, RIC, RIF, RIP, RIS, RME, SDQ, SIA (early larva), SIB (early larva), SMB (early larva), SMD (early larva), URA, URB, VAn, VBn, VCn, VDn, M5, I1, I6, NSM. Expression in the reproductive system: In adult stage, expressed in vulval muscle, uterine muscle, HSN, VCn. In developing larva stage, expressed in HSN, VCn, and anchor cell.

• Primary Identifier: Expr15443

• Primary Identifier: Expr2987
• Remark: Reporter gene fusion type not specified.
• Pattern: Expression of gcy-35 was consistently observed in URX, AQR, and PQR sensory neurons; gcy-35 expression was also observed in ALN, SDQ and BDU neurons and variably in AVM, PLM and PLN neurons, pharyngeal and body wall muscles, and the excretory cell.

• Primary Identifier: Expr15657

0 Life Stages

3 Parents

Definition	Name	Synonym	Primary Identifier
neuron of lumbar ganglion, left.	lumbar left ganglion neuron		WBbt:0005097
Neuron class of two neurons with cell bodies situated in the lumbar ganglion.	PLN		WBbt:0006855
post-embryonic cell of pedigree TL.pppa	TL.pppa		WBbt:0007379