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Picture: Figs. 4A-D. |
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Expr4836
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In hermaphrodites, the expression of bro-1 was restricted to seam cells. Its expression was first detected at bean-stage embryos and persisted throughout the developmental stages. In the male tail, bro-1 was also expressed in the ray precursor cells. |
GFP::BRO-1 was localized to both the cytoplasm and the nucleus. |
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Picture: Fig 3, Fig S1, S2, S3. |
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Expr9091
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The expression pattern of MADD-2::GFP in animals carrying trIs31 was characterized in detail and is similar to the pattern observed in animals carrying trIs32. Weak MADD-2::GFP expression was first observed in the ventral blast cells during late gastrulation, followed by stronger expression in the overlying ventral hypodermal blast cells during enclosure. MADD-2::GFP expression can also be seen in myoblasts at the two-fold stage of development and persists in the BWMs throughout the life of the animal. From the three-fold stage of embryonic development, MADD-2::GFP is localized to the right side of the ventral hypodermal ridge and to the left side of the dorsal hypodermal ridge, where the ventral and dorsal major nerve cords, respectively, reside. MADD-2::GFP is also expressed in the vulval muscles, the anchor cell, the six ventral uterine precursor cells, the lateral seam cells, and the ray precursor cells and their descendents in males. Other ectodermal derivatives that express MADD-2::GFP include the hermaphrodite-specific neurons (HSNs), and some Q cell descendents, including the AVM, PVM, SDQr, and SDQl neurons. |
Authors also expressed CFP-tagged MADD-2 in select body muscles by using the him-4 promoter. The him-4 promoter drives expression in the distal BWMs of each quadrant and not within the nervous system. In these transgenic animals, MADD-2::CFP is localized to the dense bodies and the muscle arm termini in a pattern that recapitulates MADD-2::GFP localization in strains carrying trIs31. Transgenic BWMs that are surrounded by nontransgenic cells show nearby localization of MADD-2::GFP at the hypodermal ridge, suggesting that MADD-2 is localized postsynaptically and not within axons. Within the BWMs, MADD-2::GFP is localized to the dense bodies that anchor thin fila ments to the extracellular matrix. |
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240 minutes of development (author) = late cleavage stage embryo (curator) Lineage expression: Rn descandents. |
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Expr1034
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Except for an earlier onset of GFP expression from both evpPRII.67 and evpPRII.75, all reporters displayed very similar expression patterns. Embryonic GFP expression reported by evpPRII.14 is first visible at approximately 240 minutes of development and is ubiquitous, a pattern that persists until hatching. At the beginning of each larval stage, dividing seam cells express mab-20::GFP, as do both daughter cells in males and hermaphrodites. After the anterior daughter fuses with surrounding hyp7, mab-20::GFP expression is downregulated or shut off in the both daughters. Other hypodermal expression is not evident in males or hermaphrodites. Other cells that express mab-20::GFP in hermaphrodites include the hermaphrodite-specific neurons at the L4 stage, vulva cells A to F throughout development, the migrating distal tip cells during the L4 stage, and several unidentified neurons within the nerve ring and ventral nerve cord. Expression in males include cells in the posterior ganglia, the migrating male linker cell beginning at the L3 to L4 transition, and the same nerve ring and ventral nerve cord expression observed in hermaphrodites. About 35 hours after hatching, mid-L3 males express mab-20::GFP in the 9 R(n) cells that give rise to the ray precursor clusters. Interior neuronal ganglia also express lower levels of mab-20::GFP at this stage. At 38-40 hours, when ray papillae are just visible, the 9 R(n)s and their descendents express mab-20::GFP. However, the underlying neural ganglia express the same level of GFP as the ray precursors at this stage. This pattern of expression continues until adulthood. |
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Picture: Figure S2. |
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Expr8078
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In ncEx[lin-32p::egfp] L3 males, EGFP expression is predominantly detected in epidermal ray precursor cells in their tails. |
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Cell_group: ray precursor cells and ray sublineages. This rescuing GFP reporter contains the endogenous rnt-1 3'UTR that was absent from the reporter described by Nam et al (see Expr1819). It is possible that this difference in reporters accounts for the slight difference in the rnt-1 expression pattern observed. |
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Expr3752
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rnt-1::GFP is visibly expressed in the nuclei of seam cells in embryos from around 260 minutes post fertilisation, just after the time at which seam cell are formed. Seam cell expression in both males and hermaphrodites is visible during all developmental stages, but is particularly strong until late L2. In males, rnt-1::GFP is expressed additionally in seam cell derived ray precursor cells and ray sublineages. rnt-1::GFP is also expressed transiently in body wall muscle nuclei from late embryogenesis until the end of L2. rnt-1::GFP expression was not observed in any other cell types. Expression of rnt-1 has been previously reported in intestinal cells, but no intestinal expression was found using this rescuing GFP reporter construct. |
Expressed in nuclei. |
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Lineage expression: Rn descandents. Lineage expression: SM lineage. |
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Expr2435
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HLH-2 was not detectable in the seam cells that give rise to the ray precursor cells before the mid-L3 stage. Expression in the seam was first apparent in each of the nine Rn cells; upon the division of these cells, HLH-2 could be detected only in the anterior daughter, the ray neuroblast. Later expression of HLH-2 in ray sublineage cells(probably both Rn.aa and Rn.ap) was also observed, but unable to unambiguously identify individual cells because of the higher levels of HLH-2 expression directly underlying the hypodermis, in the male sex muscle cell lineages. |
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Expr2320
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Expressed in all 18 ray neuroblast (Rn) cells in L3 males beginning slightly later than mab-3::gfp, but unlike mab-3::gfp, it is not expressed in the lateral hypodermis. See Expr950 for mab-3::gfp expression pattern. |
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Lineage expression: Rn descandents. |
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Expr2434
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Previous study show that lin-32 is expressed in Rn cells. These constructs suggest that the lin-32 promoter may continue to be active in the nine Rn.a neuroblasts and their progeny, Rn.aa and Rn.ap. GFP fluorescence was observed in cells of the next generation. It is possible that this latter expresssion could result from perdurance of GFP. However, GFP perdurance in Rn.p was seen, which has a fluorescent signal only for a short period of time after Rn division. |
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