WormMine: ExpressionPattern Expr4812

• Pattern: RDY-2 has a distribution very similar to that of VHA-5 in the excretory canal and the epidermis. Specifically, expression was seen in the lining of the excretory canal and excretory duct, but not around the excretory pore. As no expression was seen around the excretory cell body, authors conclude that RDY-2 is localized at the apical side of the canal, as reported for VHA-5 (see Expr4811). RDY-2 had a distribution in the epidermis similar to that of VHA-5 and was also excluded from seam cells. RDY-2 expression became progressively fainter after the L1 stage. In addition, authors found RDY-2 expressed in the sheath cells associated with head and tail sensory organs. Three-dimensional reconstructions showed that VHA-5 and RDY-2 formed a sixfold symmetrical pattern, which includes a larger spot that presumably corresponds to the amphid.
• Primary Identifier: Expr4812
• Remark: Picture: Figure 7A, Figure 7, C and D.
• Subcellular Localization: Localized at the apical side of the excretory canal. In the amphid sheath cell, VHA-5 and RDY-2 were found in the most distal part of the cell lining the sheath pocket, which can be equated to its apical side.

3 Anatomy Terms

Definition	Name	Synonym	Primary Identifier
a structural (glial) cell which forms an specialized environment surrounding the sensory ending(s) of one or more neurons; sometimes accompanied by a more distal socket cell. In early development of the sensory nerves and of the nerve ring some sheath cells (cephalics and labials) may also provide a substrate for axon guidance	neuronal sheath cell	pocket cell	WBbt:0005811
entire syncytium of hyp7	hyp7 syncytium	hyp7	WBbt:0005734
Four processes (canals) of the excretory canal cell each contain a central collecting lumen which feeds to a central lumenal canal in the cell body; the central canal forms a specialized membrane to release fluids into the excretory duct. These five canals form a continuous H-shaped channel which extends almost the full length of the body, generally in contact with the lateral hypodermis and the pseudocoelom.	excretory canal	canal	WBbt:0005775

1 Genes

WormBase Gene ID	Gene Name	Sequence Name	Organism
WBGene00004328	rdy-2	F53F4.6	Caenorhabditis elegans

3 Life Stages

Remark	Definition	Other Name	Public Name	Primary Identifier
	The second stage larva. At 25 Centigrade, it ranges 25.5-32.5 hours after fertilization, 11.5-18.5 hours after hatch.		L2 larva Ce	WBls:0000027
	The first stage larva. At 25 Centigrade, it ranges 14-25.5 hours after fertilization, 0-11.5 hours after hatch.		L1 larva Ce	WBls:0000024
	The whole period of embryogenesis in the nematode Caenorhabditis elegans, from the formation of an egg until hatching.		embryo Ce	WBls:0000003