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Expr4684
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GFP expression was detected at most developmental stages, with the spatial expression depending on the developmental stage of the animal. Neuronal expression of hlh-29 was detected in larvae and adults in both amphid and phasmid sockets, in the ALA and PVT neurons, in the chemosensory and mechanosensory neurons, ASI, ASK, PHA, and PQR, and in neurons of the anterior pharyngeal bulb. Weaker expression was also detected in the ASG chemosensory neurons in some transgenic lines. L1 animals show strong expression of hlh-29 in intestinal cells, and weaker expression in the rectal glands and the pharyngeal muscle cell PM1. By L3 stage, intestinal expression of the hlh-29::GFP is limited to the posterior intestinal cells, and PM1 expression is no longer detected. Expression is also detected in the ventral posterior coelomocytes in the later L3-stage larvae, and in the spermatheca and vulval muscles of L4 and adult animals. |
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Expr11622
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Expression of ceh-34::gfp transgene began during embryogenesis. CEH-34::GFP was localized to the nuclei of expressing cells. During embryonic morphogenesis and larval development and throughout adulthood, expression of the ceh-34::gfp transgene was seen predominantly in pharyngeal cells. The ceh-34::gfp transgene was expressed in all pharyngeal neurons (M4, I1, MI, I3, M3, NSM, MC, I2, I4, I5, I6, M1, M2, and M5), some pharyngeal muscle cells (pm1 and pm2) and pharyngeal epithelial cells (e1 and e3), and some body wall muscles around the anterior pharynx. |
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This Expr_pattern is about CeTMIV, an isoform of tmy-1 transcription. To confirm the CeTMIV isoform expression pattern, corresponding tmy-1::gfp vectors were assayed. Similar GFP expressions induced in pharynx and intestines respectively. These results show that the CeTMIV isoform was expressed in the pharyngeal muscles and intestinal cells and establish that the primary promoter region was located within 853 bp upstream of the initial ATG. pretzel stage (author) = fully-elongated embryo (wjc). |
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Expr1679
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The constructs pTMZIV4349 and pTMZIV1957 induced beta-galactosidase expressions in both the pharyngeal muscles and intestinal cells with similar intensities. Specifically, pharyngeal expressions were observed in all the eight muscle cells (m1-m8), one marginal cell (mc), one epithelial cell (e1) and the four cells of the pharyngo-intestinal valve (PIV). The 20 intestinal cells, some of which are binucleated and localized alongside the intestine, posterior of pharynx and anterior of anus were all stained with intense staining occurring at the most posterior end. Intestinal staining was limited only to intestinal cells, although there were slight variations in position of nuclei and staining intensity. Expression was also detected in embryos between gastrulation and the comma stage. At this stage of development, the exact nuclei are difficult to identify, but the positions and topology suggest pharynx and intestines. By the pretzel stage, identification of the different pharyngeal muscles showing expression becomes possible. A similar expression was also observed in the pharynx of males, although the intestinal staining was more restricted to the posterior region. No expression was induced by the further deletion construct pTMZIV1219. In all cases, the most uniform and intense expression patterns were observed between L1 and L2 worms, and were completed by L2. From L3 to adult, there was a reduction in expression intensity. Both pharyngeal and intestinal expressions were evident within six hours after staining. |
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Other strain-- UL990 |
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Expr2076
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Expression is seen from early larval stages until adulthood. Strongest expression is seen in the pharyngeal musculature (expression is seen in all muscle cells, excluding m6 and m7, but including m8). Weak expression is seen in a few cells in the head and tail, which are probably neuronal. |
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Identical staining pattern observed with both antisera (ST::CEH-22 and poly-his::CEH-22 ). Reporter_gene X-gal staining identical to anti-CEH-22 antibodies (Construct contains ~4 kb of ceh-22' -flanking DNA fused to lacZ within the ceh-22 5'-UTR.) This information was extracted from published material (Archana Sharma-Oates, Andrew Mounsey and Ian A. Hope). see Expr748 for western analysis data. |
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Expr603
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Antibody staining limited to nuclei within pharynx and is detected from beginning of morphogenesis onwards. CEH-22 first detected approx. 330 minutes after fertilization (the lima bean stage) in 11-14 pharyngeal muscle nuclei (probably pharyngeal muscle). At 1.5-fold stage, 14-23 pharyngeal nuclei stain. Cells identified as m3, m4, m5 and m7. In embryos that have completed elongation (pretzel stage), CEH-22 positive nuclei are identified as m3, m4, m5 and m7. Also detected in 6 other pharyngeal nuclei, which are believed to be m1 muscles. After hatching, staining persists in m1, m3, m4, m5 and m7 but absent in m6 and m2. |
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Reporter gene fusion type not specified. This Expr_pattern is about CeTMIII, an isoform of tmy-1 transcription. |
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Expr1680
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The constructs pTMZIII4135 and pTMZIII1743 permanently induced beta-galactosidase expression in pharyngeal muscles and intestinal cells of the worm from L4 stage. Pharyngeal expression was observed in m1, m3, m4, m5 and m7 muscle cells. Between the L2 and L3 stages, both constructs induced expression in tissues corresponding to germ-line tissue of the gonadal primordium: one anterior and one posterior. At this stage, intestinal expression was restricted to the most posterior part of the worm. The expression in the germ-line tissue was eliminated by L4 stage. The beta-galactosidase expression of CeTMIII isoform was stage specific. In about 95 % of L1 worms, expression was observed in the pharynx only; between L2 and L3 stages, the expression extended further to germ-line tissue and intestinal cells. Permanent expressions in pharynx and intestines were evident from L4 stage and continued to adulthood. The pharyngeal staining was much stronger than those of germ-line tissue and intestines. Unlike CeTMIV isoform, embryonic expression of CeTMIII isoform was absent and the expression intensity of intestinal cells did not decrease with stage. |
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Picture: Fig 3. |
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Expr8678
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Expression in the alimentary canal: Strong and consistent expression in pharyngeal epithelium, pm1, pm2, pm3, pm6, pm7, pm8, mc1, mc2, mc3. Weak or rare expression in pm4, pm5. Expression in the nervous system: DDn, DVA, DVB, DVC, PVP. Expression in the reproductive system: In adult stage, expressed in proximal gonad sheath, spermatheca. In developing larva stage, expressed in uterus, spermatheca. inx-10 is localized to pharyngeal precursors from early stages of embryogenesis, and by three-fold stage, all pharyngeal muscles except pm4 are seen to express it at high levels. |
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Expr15821
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We first used the gpa-16 promoter (Jansen et al., 1999) to broadly drive the expression of GCaMP3 in pharyngeal muscle. We characterized the expression pattern of this transgene using confocal microscopy and observed bright fluorescence in the pm2 and pm3 muscles and also in the mc1 marginal cells. We also observed occasional dim fluorescence in pm1 (3 of 10 animals). |
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Picture: N.A. |
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Expr8674
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Expression in the alimentary canal: Strong and consistent expression in pharyngeal epithelium, pm1, pm2, pm3, pm4, mc2. Weak or rare expression in pm6, vir. Expression in the nervous system: AVD, AVK, RIS, URB. Pharyngeal and neuronal expression of inx-6 start around threefold stage, and some of the expression in head and tail neurons disappears after L1 stage. |
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Picture: N.A. |
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Expr8675
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Expression in the alimentary canal: Strong and consistent expression in pm5, MC. Weak or rare expression in pharyngeal epithelium, pm1, pm2, pm3, pm4 pm6, pm7, pm8, g1, g2, rectal gland cells. Expression in the nervous system: ADE, AIY, ALM, ALN, AVA, AVK, AVM, BDU, CAN, DAn, DVA, DVB, DVC, FLP, HSN, LUA, PLM, PLN, PVC, PVM, PVP, PVQ, PVT, PVW, RID, RIS, SDQ, URB, MC. Expression in the reproductive system: In adult stage, expressed in HSN. Faint hypodermal expression of inx-7 is seen around two-fold stage and becomes stronger by threefold stage. |
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Picture: Fig 3. |
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Expr8676
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Expression in the alimentary canal: Strong and consistent expression in pm1, pm3. Weak or rare expression in pharyngeal epithelium, pm2, pm4, pm5, pm6, pm7, pm8, intestine, rectal epithelial cells. Expression in the nervous system: ILso, AIN, AVF, AVJ, AVK, PVR, SAB. Expression in the reproductive system: In adult stage, expressed in Gonad sheath, Vulva(low), vulval muscle, uterine muscle. In developing larva stage, expressed in vulval muscle, uterine muscle. inx-8 is expressed broadly, albeit at very low levels, around two-fold stage, and its expression becomes stronger in the pharynx, nervous tissue, HMC, and GLR cells as development continues. In the reproductive system, expression of inx-8 starts during early larval stages and continues during migrations of the great granddaughters of the SM blast cell to their final locations and after the sex muscles achieve their final structures. inx-8 is expressed in the hypodermal cells of the animal in postembryonic stages. |
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Picture: Fig 3. |
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Expr8679
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Expression in the alimentary canal: Strong and consistent expression in anterior arcades, posterior arcades, pharyngeal epithelium, pm4, pm8, g1, g2, vir, K.a/K' cells. inx-11 is more strongly expressed in the most posterior (int 9) intestinal cell. Weak or rare expression in pm1, pm2, pm3, pm5, pm6, pm7. Expression in the nervous system: CEPsh, DVC, LUA. Expression in the reproductive system: In adult stage, expressed in utse. In developing larva stage, expressed in uterus, sperm (spermatocytes, spermatids). Expression of inx-11 appears in pharyngeal tissue around two-fold stage, and by three-fold stage, strong expression becomes restricted to g1, g2, pm4, and pm8. inx-11 is expressed in the hypodermal cells of the animal in postembryonic stages. |
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Feature : "myo-2.B". |
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Expr11413
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The B+B enhancer (pOK17.13) activates frequent expression only in pharyngeal muscles m3, m4, m5 and m7, with occasional expression in m1. No expression has been observed in m6 or m2. |
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Picture: N.A. |
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Expr8688
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Expression in the alimentary canal: Strong and consistent expression in pm1, pm2, pm8, vir. Weak or rare expression in pharyngeal epithelium. inx-20 appears in pm1, pm2, pm8, and intestinal rectal valve at threefold stage and continues to adulthood. |
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[ser-2::gfp] transcriptional fusion constructs. Ser-2 reporter constructs were generated by using a PCR fusion protocol, using pPD95.75 as a template for green fluorescent protein (gfp). For all gfp fusion primers listed, gfp vector sequence is indicated in lowercase, and gene-specific sequence is indicated in uppercase. [ser-2::gfp] translational fusion. A translational fusion of the whole ser-2 locus to gfp was created by using an in vivo recombination technique. Specifically, two overlapping PCR fragments, one containing the 5' part of a locus, the other containing the remainder of the locus PCR-fused to gfp, were coinjected into the worm. Recombination of these two fragments via the homologous region leads to the expression of a full-length ser-2::gfp fusion. |
Expr2707
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Expression using the upstream regulatory regions of exon 1bc (ser-2prom2::gfp) is mostly restricted to the AIYL/R, AIZL/R, RID, DVA, BDUL/R, SIADL/R, and SIAVL/R interneurons. Less consistent expression is observed in PVT. In addition, expression is observed in the RMEL/R motor neurons. Outside the nervous system, expression can be observed in the excretory gland cells. No more transcriptional regulatory information is contained within intronic regions by generating a fusion of gfp to the full coding genomic ser-2 locus using an in vivo recombination technique([ser-2::gfp] translational fusion. Transgenic animals expressing such a construct show an expression pattern similar to the one observed with the ser-2prom1::gfp construct. The upstream regulatory region of the third splice form, containing exon 1d(ser-2prom3::gfp), drives expression exclusively in two sensory neuron classes, OLL(L/R) and PVD(L/R). ser-2prom1::gfp is expressed in the AIY interneuron class and a set of unidentified neurons. These neurons were identified as head and tail interneuron classes, namely AVHL/R, AUAL/R, AIYL/R, RICL/R, SABVL/R, RID, RIAL/R, SABD, SDQ, CANL/R, DA9, LUAL/R, ALNL/R, and PVCL/R. In addition to its expression in neurons, ser-2prom1::gfp is also expressed in pharyngeal cells (NSM neurons and pm1/6 muscles) and in head muscles. In males, expression can be observed in posterior dorsal and ventral body wall muscles, the male-specific diagonal muscles, and several posterior neurons likely to be CP neurons. |
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Confirmation of GFP staining and NCS-1-positive cells was obtained with antibodies against Ce-NCS-1. See Expr893. |
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Expr892
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Ce-NCS-1 was predominantly expressed in sensory neurons (10 neuronal pairs: AWC, ASE, AWB, BAG, PHB, AWA, AFD, ADF, ASG, PHA). In addition, two pairs of interneurons (AVK, AIY), one motor neuron (RMG), and one muscle cell type (pm1) expressed Ce-NCS-1. Most of the NCS-1-expressing neurons were associated with two sensory organs, the head amphids and tail phasmids |
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Feature : [ceh-22DE2::pPD95.27] |
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Expr11803
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The distal enhancer DE2 was highly active in the m1 pharyngeal muscle cells, a specialized syncytial cell forming a thin sheet at the anterior of the pharynx. |
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Expr11322
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Adult: strong expression in vulval epithelium, gut, head muscle, pharynx: pm1,6,7,8, I3(?), a few more pharyngeal cells (neurons? epithelium); some arcade cells, excretory duct cell, 4 neurons in the retrovesicular ganglion, rectal glands, one additional rectal cell; occasionally hyp (expresses strongly in young larvae) |
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Expr12198
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Examination of the expression pattern of prdx-2 using a rescuing translational reporter (prdx-2prom::prdx-2::mCherry) revealed expression in a broad set of tissues: I2, I4, and intestine (as previously reported; Isermann et al., 2004), as well as muscle (pharyngeal muscle 1, vulval muscle, body wall muscle), epithelial cells (e1, e3), and many neurons in the head and tail. |
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Both [des-2::gfp] and [des-2::lacZ] show the same pattern. Reporter gene fusion type not specified. Similar expression pattern to deg-3 except for lack of expression in touch receptors. This may be because the des-2 construct lacks intron 8 and 9 and the 3' UTR of des-2 may lack the necessary elements for touch expression. This information was extracted from published material (Archana Sharma-Oates, Andrew Mounsey and Ian A. Hope). |
[des-2::gfp]. gfp construct has a 5kb BamHI-XbaI fragment (from plasmid containing the deg-3 operon) inserted into plasmid pPD95.75. The gfp gene was fused to the intracellular loop of des-2 in this construct. |
Expr627
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Expressed in m1 head muscles, IL2 neurons, FLP neurons, PVD neurons and PVC neurons. |
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Picture: N.A. |
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Expr8669
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Expression in the alimentary canal: Strong and consistent expression in pm1. inx-1 starts to be expressed in a few head neurons including AIB (brightest) and AIY (fainter; expression ends in L2) by three-fold stage. |
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