WormMine: ExpressionPattern Expr4783

• Pattern: Prominent expression of ORAI-1::GFP was detected in the spermatheca, intestine and hypodermis. In the intact animal, it was unclear whether gonadal sheath cells expressed ORAI-1::GFP, due to the intense fluorescence from the intestine and spermatheca. To examine sheath cell expression further, authors imaged gonads dissected free from a worm strain expressing an orai-1 transcriptional GFP reporter. orai-1 is also expressed in both proximal and distal gonadal sheath cells.
• Primary Identifier: Expr4783
• Subcellular Localization: Intestinal expression appeared to be localized to both apical and basolateral membrane regions. The circumferential ORAI-1::GFP localization pattern is consistent with expression in the basal plasma membrane. Non-circumferential localization is probably due to membrane folding and/or expression at lateral cell borders. The complicated morphology of the spermatheca precluded definitive localization of ORAI-1::GFP to the apical cell membrane.

4 Anatomy Terms

Definition	Name	Synonym	Primary Identifier
A chain of very large cuboidal cells forming a wide central lumen in which food arrives from the posterior pharynx, is digested, and from which waste products proceed to the rectum. Intestinal rings form in groups of two and four cells surrounding the common lumen; thus the epithelium is only one cell deep at any point, with neighboring cells firmly secured to their neighbors by apical adherens junctions. These cells have very large nuclei and many large vacuoles, yolk granules, and other inclusions; the latter increase in number and electron density as the animal ages.	intestine	gut	WBbt:0005772
an accordion-like tube that contains sperm and is the site of oocyte fertilization.	spermatheca		WBbt:0005319
Epidermal layer.	hypodermis	epidermis	WBbt:0005733
five pairs of thin gonadal sheath cells form a single layer covering the germ line component of each arm, each pair occupying a stereotyped position along the gonad proximal-distal axis.	gonadal sheath cell		WBbt:0005828

1 Genes

WormBase Gene ID	Gene Name	Sequence Name	Organism
WBGene00015648	orai-1	C09F5.2	Caenorhabditis elegans

0 Life Stages