WormMine: AnatomyTerm WBbt:0003938

• Definition: Neuron class of one ring motoneuron, projects along dorsal cord, innervates dorsal body muscle.
• Name: RID
• Primary Identifier: WBbt:0003938
• Synonym: lineage name: ABalappaapa

1 Children

Definition	Name	Synonym	Primary Identifier
nucleus of pedigree ABalappaapa	ABalappaapa nucleus		WBbt:0002707

6 Expression Clusters

Regulated By Treatment	Description	Algorithm	Primary Identifier
	Top 300 transcripts enriched in RID according to single cell RNAseq.	Top 300 enriched transcripts were determined by log2.ratio of the tpm in the cell type vs the tpm in the other cells * the log2 of the cell.type tpm.	WBPaper00061340:RID
	Single-cell RNA-Seq cell group 84 expressed in: Unassigned uterine cells.	CellRanger, DecontX, Monocle3, Louvain algorithm.	WBPaper00065623:84
	Genes enriched in RID neurons. Authors compared genes enriched in FACS sorted GFP+ cells (RID, ALA, AIY, CAN and a sheath cell) vs. whole animal in WT hpIs202[Pceh-10GFP] (WT enriched sets), and genes enriched in FACS sorted GFP+ cells (ALA, AIY, CAN and a sheath cell) vs. whole animal in unc-39(hp701) hpIs202[Pceh-10GFP] (unc-39 enriched sets) at L2 larva stage. This set of genes are WT enriched but not unc-39 enriched, suggesting they are enriched in RID neurons.	DESeq	WBPaper00050496:RID_enriched
	Single-cell RNA-Seq cell group 129_0 expressed in neuron.	scVI 0.6.0	WBPaper00065841:129_0
	Transcripts enriched in RID according to single cell RNAseq.	Genes that pass the Bonferroni threshold for multiple comparisons (q < 0.05) are significantly enriched.	WBPaper00061651:RID_enriched
	Single-cell RNA-Seq cell group 83 expressed in: RID.	CellRanger, DecontX, Monocle3, Louvain algorithm.	WBPaper00065623:83

72 Expression Patterns

• Primary Identifier: Expr4784
• Pattern: Three independent transgenic lines display GFP fluorescence exclusively in VB motor neurons in the ventral nerve cord. ceh-12::GFP, is expressed in all 11 VB motor neurons and in a single head neuron, RID; occasional weak GFP expression was observed in the pharyngeal/intestinal valve cell and in the excretory gland cell.

• Primary Identifier: Expr4382
• Pattern: Expressed in AVA, RIB, RID, SIB, SMD, CEP(?), ADE(?), some unidentified neurons in the head and tail. Also expressed in anal depressor, head muscles (strong), body wall muscles (strong).

• Primary Identifier: Expr4381
• Pattern: Expressed in AVA, AVB, DVA, I5, RID, RIM, PVQ, SAA, SIA, SIB, SMB(?), SMD, ventral cord motor neurons, some unidentified neurons in the head. Also expressed head muscles (weak), body wall muscles (weak).

• Primary Identifier: Expr15649

• Primary Identifier: Marker49
• Remark: Picture: N.A. Reporter gene fusion type not specified.
• Pattern: Expressed in anterior neurons, including AIY, AIZ, RID, M5, ASI, and labial sensory neurons, VNC motorneurons, midbody neurons HSN, CAN, and PVM, tail neurons DVB, DVC, and PDB, and the nonneuronal excretory cell, uterine muscles. -- according to pers. comm. from Oliver Hobert.

• Primary Identifier: Marker63
• Remark: Picture: N.A. Reporter gene fusion type not specified.
• Pattern: RID cell fate marker

• Primary Identifier: Marker64
• Remark: Picture: N.A. Reporter gene fusion type not specified.
• Pattern: RID cell fate marker

• Primary Identifier: Marker65
• Remark: Picture: N.A.
• Pattern: RID cell fate marker

• Primary Identifier: Expr15558

• Primary Identifier: Expr15571

• Primary Identifier: Expr15572

• Primary Identifier: Expr15573

• Primary Identifier: Expr15579

• Primary Identifier: Expr15586

• Primary Identifier: Expr15651

• Primary Identifier: Expr15652

• Primary Identifier: Expr15589

• Primary Identifier: Expr13158

• Primary Identifier: Expr15591

• Primary Identifier: Expr13164
• Pattern: For lgc-38, all expressing cells shown are observed with the 3.5 kb reporter fusion, except for OLL, which only expresses the 3.9 kb fusion; URA expresses both.

• Primary Identifier: Expr15598

• Primary Identifier: Expr15604

• Primary Identifier: Expr14590
• Pattern: Embryonic expression of exc-7 was first observed at the bean stage. By reverse lineaging with use of SIMI-Biocell software, we confirm the identity of one of the expressing cells at this stage as the excretory canal cell. In L1 animals, broad expression in the head, ventral nerve cord (VNC), and tail was observed. In young adults, expression is notably observed in vulva cells. In the nervous system specifically, expression is observed in many neurons throughout the body, but unlike Drosophila Elav, exc-7::gfp it is not panneuronally expressed. We confirmed previously reported expression in cholinergic VNC MNs, but absence of GABAergic VNC MNs, consistent with previous reports (Fujita et al., 1999; Loria et al., 2003) and consistent with exc-7 functioning in cholinergic, but not GABAergic neurons to control alternative splicing (Norris et al., 2014). exc-7::gfp is also expressed in some non-neuronal cell types, including muscle and hypodermis, but not in the gut. A previous report showed that exc-7 is only transiently and weakly expressed in the excretory cell, which, based on exc-7's excretory mutant phenotype, has puzzled researchers (Fujita et al., 2003). We find that the gfp tagged exc-7 locus is strongly and continuously expressed in the excretory canal cell.

• Primary Identifier: Expr15608

• Primary Identifier: Expr15611

• Primary Identifier: Expr13662
• Pattern: unc-39 reporters were expressed by muscles and multiple neurons during embryonic development and into the adulthood. Notably, robust expression of unc-39 reporters was observed in the embryonic RID precursor, embryonic RID, and newly hatched L1 larvae. Post-embryonically, unc-39 expression was selectively decreased in some neurons, including RID.

• Primary Identifier: Expr3136
• Pattern: C33G8.5 showed medium to strong expression in scattered nonchemosensory neurons.5

• Primary Identifier: Expr13664
• Pattern: ins-17 is robustly expressed in RID and additional neurons.

• Primary Identifier: Expr3222
• Remark: Reporter gene fusion type not specified.
• Pattern: Epidermis: ncr-1Ap(l)::gfp was strongly expressed in the excretory cell and rectal epithelial cells from the early L1 larval stage and through all life stages. Seam cell expression was first observed in the late L1 stage, while expression in the lateral hypodermis increased during the L3 stage and peaked during the L4 stage. Seam cell and hypodermal expression gradually decreased in the adult stage and was hardly visible among older adults. ncr-1Ap(s)::gfp was not expressed in the hypodermis under normal growth conditions, though lateral hypodermal but not seam cell expression was dramatically upregulated in starved animals of all developmental stages. No increase in hypodermal expression was seen in starved ncr-1Ap(l)::gfp animals. Intestine: Both ncr-1Ap(s)::gfp and ncr-1Ap(l)::gfp were strongly expressed throughout the intestine, with posterior intestinal expression consistently stronger than anterior expression. Musculature: ncr-1Ap(l)::gfp was strongly expressed in pharyngeal muscles, but not in body wall muscles. Nervous system: ncr-1Ap(s)::gfp and ncr-1Ap(l)::gfp were expressed in the same set of head and tail neurons and a pair of neuron-like cells identified as the XXX cells. According to their location and cellular morphology, the head neurons were identified as the pharyngeal neuron I6, the inner labial sensory neurons IL2s and the amphid neurons ASE and ASG. The expression level in the amphid neurons was weaker than in the other head neurons. The tail neurons were identified as PHC, in which expression was first detected during the L2 stage, consistent with the time of birth of the neurons at the end of L1. In contrast to the widespread expression of ncr-1Ap::gfp, ncr-1Bp::gfp is expressed exclusively in 10-12 pairs of head and tail neurons. The tail neurons were identified as PHA, PHB and DVC. One pair of head neurons was identified as AWC. The other head neurons were very tentatively identified as RIC, RIM, FLP, ADA, ADE, RID and maybe AIY. We also occasionally observed expression in a pair of head cells anterior to the nerve ring. The position and morphology of these cells are similar to the XXX cells. With the exception of PHC neurons, expression in the tissues above was first observed during late embryogenesis and did not change during development. Somatic gonad: Both ncr-1Ap(s)::gfp and ncr-1Ap(l)::gfp were strongly expressed in the spermatheca and weakly in the gonadal sheath cells. The expression in the somatic gonad could be observed only in adults. Three reporter constructs, ncr-1Ap(s)::gfp, ncr-1Ap(l)::gfp and ncr-1Bp::gfp were made for ncr-1 because of its complex gene structure. The results indicate that ncr-1 expression is widespread and largely coincides with the reported distribution pattern of cholesterol in C. elegans, which includes the following tissues: intestine, pharynx, excretory gland cell, nerve ring, spermatheca and germ cells, including both oocytes and sperm.

• Primary Identifier: Expr7836
• Remark: In snf-11(ok156) mutants, anti-SNF-11 staining was completely absent, confirming the specificity of the staining. Picture: Fig 4.
• Pattern: In healthy young adults, the anti-SNF-11 antibody strongly stained the four RME neurons (RMED, RMEV, RMEL, and RMER). Faint staining of three additional GABAergic neurons (AVL, DVB, and RIS) was sometimes observed. Several non-GABAergic neurons, including RID, also seemed to stain. The ventral nerve cord DD and VD inhibitory motor neurons did not stain. Faint staining of the body wall, anal, and uterine muscles with the anti-SNF-11 antibody was observed in some animals.
• Subcellular Localization: Staining of both the processes and the soma of each neuron were observed. In RMED and RMEV, a punctate staining pattern was observed in the posteriorly directed processes, possibly corresponding to synapses.

11 Life Stages

Remark	Definition	Other Name	Public Name	Primary Identifier
	The whole period of embryogenesis in the nematode Caenorhabditis elegans, from the formation of an egg until hatching.		embryo Ce	WBls:0000003
	The C. elegans life stage spanning 620-800min(hatch) after first cleavage at 20 Centigrade. Cell number remains at ~560 cells, with some new cells generated and some cells go through programmed cell death. A stage after elongation is over. The last stage of embryogenesis. Also called pre-hatched embryo, late embryo or morphogenetic embryo.		fully-elongated embryo Ce	WBls:0000021
	The C. elegans life stage spanning 350-620min after first cleavage at 20 Centigrade. Cell number remains at ~560 cells, with some new cells generated and some cells go through programmed cell death. The stage that embryo starts elongation until elongation is over.		elongating embryo Ce	WBls:0000015
	The C. elegans life stage spanning 290-350min after first cleavage at 20 Centigrade. Proliferate from 421 cells to 560 cells. The stage when embryo just finished gastrulation and is enclosing.		enclosing embryo Ce	WBls:0000013
	The C. elegans life stage spanning 0-350min after first cleavage at 20 Centigrade. Proliferate from 1 cell to 560 cells. From start of first cleavage until cleavage is over.		proliferating embryo Ce	WBls:0000004
	The C. elegans life stage spanning 520-620min after first cleavage at 20 Centigrade. Cell number remains at ~560 cells, with some new cells generated and some cells go through programmed cell death. The shape of embryo is elongated and tripple fold. A stage between 2-fold embryo and fully-elongated embryo. Also called pretzel embryo or pretzel stage.		3-fold embryo Ce	WBls:0000020
	The C. elegans life stage spanning 420-460min after first cleavage at 20 Centigrade. Cell number remains at ~560 cells, with some new cells generated and some cells go through programmed cell death. The shape of embryo is elongated and fold back 50%. A stage between comma embryo and 2-fold embryo.		1.5-fold embryo Ce	WBls:0000018
	The C. elegans life stage spanning 390-420min after first cleavage at 20 Centigrade. Cell number remains at ~560 cells, with some new cells generated and some cells go through programmed cell death. The shape of embryo looks like a comma. A stage between bean embryo and 1.5-fold embryo.		comma embryo Ce	WBls:0000017
	The C. elegans life stage spanning 460-520min after first cleavage at 20 Centigrade. Cell number remains at ~560 cells, with some new cells generated and some cells go through programmed cell death. The shape of embryo is elongated and double fold. A stage between 1.5-fold embryo and 3-fold embryo.		2-fold embryo Ce	WBls:0000019
	The C. elegans life stage spanning 350-390min after first cleavage at 20 Centigrade. Cell number remains at ~560 cells, with some new cells generated and some cells go through programmed cell death. Emrbyo elongation started but have not formed comma shape yet. The shape of embryo looks like a lima bean. A stage right before comma embryo. Also called lima embryo or lima bean stage.		bean embryo Ce	WBls:0000016
	The C. elegans life stage spanning 210-350min after first cleavage at 20 Centigrade. Proliferate from 421 cells to 560 cells. The stage before the fast cleavage of cells finishes.		late cleavage stage embryo Ce	WBls:0000014

5 Parents

Definition	Name	Synonym	Primary Identifier
neuron with its cell body situated in the head, excluding the pharynx.	head neuron		WBbt:0006751
neuron making synapse on an effector (muscle, gland).	motor neuron	motoneuron	WBbt:0005409
	interneuron		WBbt:0005113
ganglion that lies beside the nerve ring in the head. It contains neuron cell bodies that send their neuronal processes into the ring and longitudinal nerves, but form no local neuropil separate from the nerve ring. The dorsal ganglion is in close contact with the dorsal hypodermal cord.	dorsal ganglion		WBbt:0005214
embryonic cell	ABalappaap		WBbt:0006727