WormMine: AnatomyTerm WBbt:0006791

• Definition: uterine-vulval cell proximal to the vulva.
• Name: uv1
• Primary Identifier: WBbt:0006791

2 Children

Definition	Name	Synonym	Primary Identifier
uv1 cell that follows 5R lineage configuration in development.	uv1 5R		WBbt:0008222
uv1 cell that follows 5L lineage configuration in development.	uv1 5L		WBbt:0008221

1 Expression Clusters

Regulated By Treatment	Description	Algorithm	Primary Identifier
	Single-cell RNA-Seq cell group 146_0 expressed in epithelium.	scVI 0.6.0	WBPaper00065841:146_0

59 Expression Patterns

• Primary Identifier: Expr4781
• Pattern: Confocal analysis of strains containing the Punc-31::GFP transcriptional reporter revealed expression in the nerve ring, ventral nerve cord motor neurons, preanal ganglion, and head ganglion. Counts of cell bodies in the ventral nerve cord and preanal ganglion revealed an average of 65 +/- 2 (n = 4) GFP-positive cells, indicating that essentially all of the neurons of the ventral nerve cord and preanal ganglion express unc-31. Additional positive identifications were made for SDQ, PDE, BDU, ALM, DVA, DVB, DVC, HSN, and CAN neurons. Because these encompass most of the identifiable neurons in the background of a pan-neuronal expression pattern, authors infer that unc-31 may be expressed in essentially all neurons. All reporter strains also displayed expression in the vulval muscles VM1 and VM2 and occasional expression in what is likely VulE and VulF. Consistent expression was also noted in the UV1 cells and the spermatheca. In summary, CAPS/UNC-31 is expressed throughout the nervous system and in other secretory cells.

• Primary Identifier: Expr4976
• Remark: Picture: Figure 4A and supplemental Table S1.
• Pattern: OCR-2 reporter expression was found in sensory neurons. These sensory neurons have no known role in egg laying. In addition, OCR-2 reporter was expressed in cells of the egg-laying system. These were the four uterus-associated uv1 cells attached to the ventral surface of the uterus, as well as the syncytial uv1-associated cell utse. In adults, OCR-2 reporter expression was much stronger in uv1 than in utse; in larval animals, it was the reverse.

• Primary Identifier: Expr4266
• Pattern: Reporter genes that begin at -3190, -2021, -1248, and -517 bp upstream of the ATG of this alternate first exon 1 were expressed in body wall muscle cells, neurons in the head, nerve ring, ventral and dorsal nerve cords, neurons, and some epidermal cells in the tail. Weaker expression was also observed in pharyngeal muscles. The expression from promoter 2 started in the embryos at the 1.5-fold stage and was continuous throughout development. Expression from this internal promoter element is observed in vulval cells at the L4 stage and in adult hermaphrodites, including the uterine vulval cells uv1, 2, 3, and surrounding epithelium.

• Primary Identifier: Expr4259
• Pattern: These transgenes express in the vulF cells of the vulva, and the uv1 cells of the uterus.

• Primary Identifier: Expr15243

• Primary Identifier: Expr15244

• Primary Identifier: Expr15249

• Primary Identifier: Expr15250

• Primary Identifier: Expr15251

• Primary Identifier: Expr15253

• Primary Identifier: Expr15255

• Primary Identifier: Expr15228

• Primary Identifier: Expr15231

• Primary Identifier: Expr15232
• Pattern: Unc-64 isoform A and B.

• Primary Identifier: Expr15241

• Primary Identifier: Expr9338
• Pattern: Ags-3::GFP expression was seen in most or all adult neurons, including neurons in the head, tail, egg-laying system, along the ventral and dorsal nerve cords, and in muscles, including body wall muscles, egg-laying muscles, and pharyngeal muscles.

• Primary Identifier: Expr8613
• Remark: Picture: Fig 4A to D.
• Pattern: lgc-55::mCherry and lgc-55::GFP transgenic animals showed reporter expression in a subset of neck muscles and a restricted set of neurons.These neurons aare AVB, RMD, SMDD, SMDV, IL1D, IL1V, SDQ, HSN, and ALN neurons. In addition, weak lgc-55 reporter expression was also detected in the UV1 cells and tail muscle cells.

• Primary Identifier: Expr1424
• Remark: The expression pattern from the GFP, lacZ, and antibody studies were consistent, except that GFP reporter expression was observed in the intestine only with syntaxin C fusions. This expression was limited to the posterior half of the intestine. It is possible that sequences that direct gut expression are absent from the GFP clones since sequences further upstream of the initiation codon are present in the lacZ constructs.
• Pattern: Syntaxin A and C were expressed in neurons and in five nonneuronal tissues. By contrast, expression of syntaxin B was limited to the five nonneuronal cell types. Also stained were the uv1 secretory cells of the vulva, the excretory gland cells, the distal tip cells, the spermatheca, and the intestinal muscle. In the uv1 cells, GFP fluorescence was concentrated subcellularly.
• Subcellular Localization: In neurons, syntaxin A and C were found ubiquitously on neuronal cell bodies, axons, and dendrites.

• Primary Identifier: Expr14091
• Remark: Other Strain: OH13842
• Pattern: ASH, ASK, 1 bright neuron pair just posterior to ASI, 1 neuron pair at the level of ASH just posterior to the nerve ring, maybe other neurons? (a bit variable), VNC (dim), PHB, sometimes dim PHA, vulva muscle and uv1 cells, pharynx - In general very bright.

• Primary Identifier: Expr3776
• Pattern: In contrast, D1 and D2 were very strongly expressed in the muscles of the pharynx and vulva and in both the cell bodies and axons of a very restricted set of neurons. These included the hermaphrodite-specific neurons (HSNs), about four unidentified neurons with cell bodies in either the ventral or retrovesicular ganglion near the terminal bulb of the pharynx, and about six neurons with cell bodies in the lumbar ganglion in the tail, including PVQL and PVQR, as distinguished by their axonal patterning. A small number of axons were present in the nerve ring and the ventral cord. The lateral processes of ALNL and ALNR were also visible. D1 and D2 also exhibited sporadic and weak fluorescence in body wall and intestinal muscles. Isoform E was strongly expressed in the nervous system and was detected in the cell bodies and axons of most, if not all neurons, including those in the pharynx, and at least some of the neuron-associated sheath and/or socket cells. Isoform E was also observed in the excretory canals and the somatic gonad, including the spermatheca, gonadal sheath, and distal tip cells. Isoform B was expressed most strongly in the axons of neurons, particularly in the nerve ring and the ventral nerve cord. This pattern of expression was very similar to the staining pattern observed with UNC-73 B antibodies. UNC-73 B was also found more sporadically and at a lower level in anal depressor muscle, distal tip, P, seam, and developing vulva cells. Although C1 and C2/F were also expressed in axons, their expression was restricted to fewer neurons. Many process bundles within the body of the animal and neurons within the pharynx were positive for C1 and C2/F, but few axons were visible in the nerve ring. Along with the neuronal expression, C1 and C2/F were also expressed in neuron-associated socket and/or sheath cells and the neuroendocrine uv1 cells. A low level of expression was sporadically observed in body wall muscles.
• Subcellular Localization: Expressed in axons and cell bodies.

• Primary Identifier: Expr1960
• Reporter Gene: [sek-1::gfp] translational fusion.
• Pattern: Transgenic animals bearing the sek-1::gfp fusion exhibited fluorescence in excretory canal. rectal epithelial cells, uterine-vulval cells and several neurons.

• Primary Identifier: Expr9227
• Pattern: Expression of HID-1::GFP was observed predominantly in the nervous system, the intestine, the pharynx, and in uv1 secretory cells (data not shown). In the nervous system, HID-1-GFP was primarily localized in the synapse-rich nerve ring, ventral cord, and dorsal cord process bundles. In the nerve cords HID-1-GFP was punctate in distribution. GFP fluorescence was detected in neuronal cell bodies as well. HID-1-GFP under the hid-1 promoter was capable of rescuing the uncoordinated movement, aldicarb resistance, the egg laying, and the defecation defects of hid-1(sa722) null mutants.

• Primary Identifier: Expr7411
• Remark: Clone: pUL#JRH/AD12
• Pattern: Expression is seen in the vulval uv1 cells. In addition, weak expression is seen in head nerve cells, nerve cord and anterior body wall muscles in 2 fold embryos through to L1.

• Primary Identifier: Expr3012
• Pattern: Expressed in AUA, BAG, DA, DD, DVB, LUA, PHC, PVC, SAB, URX, VD, uv1, head muscle. Faint or variable expression observed in socket cells. Male specific expression in Ray 4.

• Primary Identifier: Expr843
• Remark: Reporter gene fusion type not specified. To analyze ida-1 gene expression in males, the pida-1::GFP transgenic strain BL5715 was crossed with the him-8 (high incidence of males) mutant strain BW1663. cgc6469 mentioned that this reporter gene is transcriptional fusion.
• Pattern: The ida-1 gene promoter-driven GFP expression pattern in males differs from hermaphrodites, especially in the tail, where many more fluorescent cells are observed. Male larvae appear very similar to hermaphrodite larvae, the only difference being that the PVP neurons in the preanal ganglion showed higher GFP expression levels. It was concluded that all neurons expressing GFP in the hermaphrodite pharyngeal nerve ring and in the tail also express GFP in males. In adult males, additional fluorescence is seen in neurons anterior to the nerve ring, in the ventral cord and many more in the tail. The four additional GFP-expressing cells in the procorpus just anterior to the pharyngeal bulb have not been identified. Expression in the male-specific CEM neurons at the anterior end of the nerve ring appeared variable. The hermaphrodite-specific cells VC, HSN, and uv1 observed to express GFP are not found in males. Nonetheless, several cell bodies in the male ventral cord expressed GFP. Dorsally directed processes emanate from them, which identifies them as CAn, a set of eight motoneurons. As in hermaphrodites, PDE weakly expressed GFP. In the male tail, about 20 neurons express GFP. They include PHA, PHB, PHC, and PVP in the preanal ganglion, which are already seen in larvae. Some of the rays contain GFP-filled axons that extend all the way to the tip of the ray. Rays 2, 8, and 9 show the highest expression levels; axons of rays 1, 4, and 6 fluoresce more weakly. No GFP expression is observed in the spicules.

• Primary Identifier: Expr842
• Remark: Reporter gene fusion type not specified. cgc6469 mentioned that this reporter gene is transcriptional fusion.
• Pattern: GFP expression was observed in about 30 neurons of adult hermaphrodite worms. pida-1::GFP 7.6 expression was prominent in a subset of neurons in the head nerve ring, around the vulva, in a few cells in the ventral cord, and in the tail. Although the GFP was concentrated in the nucleus, the whole cell body including axons was labelled. Labeled processes included two lateral and one dorsal axon and a bundle of ventral axons running the length of the body, a pair of lateral axon bundles running from the head to the tip of the nose, and a pair of axons running from the anal region to the tip of the tail. GFP expression was observed as early as in late embryos. Larvae showed expression in the head and the tail, whereas GFP expression near the vulva and in the ventral cord was only seen in late L4 and adult animals. The expression pattern in dauer larvae was similar to that in other larval stages. Males exhibited many more GFP-expressing cells in the tail and additional cells in the head, and expression in the ventral cord differed from that observed in hermaphrodites. The GFP-expressing neurons in the hermaphrodite head were identified as ADE, ALA, ASI, ASK, AUA, ASG, AVH, and AVJ. Expression in ASG, AVH, and AVJ was not as bright as in other cells and was variable from individual to individual. A few other GFP-expressing anterior neurons could not be identified unequivocally. The fluorescent cells in the midsection of the animal were identified as the vulval motoneurons VC and HSN and the vulval uv1 cells. These are all hermaphrodite-specific and GFP expression was not observed until the late L4 larval stage. A lateral pair of neurons in the posterior half of the animal with weak GFP expression was identified as PDE. They possess ventrally directed processes entering the ventral nerve cord. In the preanal ganglion a pair of neurons with weak GFP expression was identified as PVP. Three pairs of GFP-expressing cells in the tail were identified as PHA, PHB, and PHC. Corresponding cells for the dorsal axon and a preanal structure have not been identified. The latter short process originates from the ventral nerve cord and peters out and terminates at the hypodermis.
• Subcellular Localization: GFP was concentrated in the nucleus, the whole cell body including axons was also labelled.

• Primary Identifier: Expr3021
• Pattern: Expressed in AIM, ASG, AVA, AVG, AVL, CEP, PVD, PVW, RIC/AIZ, RIV, SMD, URA, uv1. Male specific expression in 6 out of 9 CP.

• Primary Identifier: Expr15414

• Primary Identifier: Expr9896
• Reporter Gene: [dcar-1::GFP] transcriptional fusion. The dcar-1 (C06H5.7) genomic region was prepared using internal primers from cosmid E01B7. For the dcar-1::GFP fusion, 4 kb of sequence upstream of the dcar-1 gene as well as DNA encoding of the first 150 aa of the predicted DCAR-1 protein were amplified by PCR and subcloned into the pPD95.77 vector in-frame with GFP.
• Pattern: In transgenic animals, dcar-1::GFP was expressed in some sensory neurons and in some non-neuronal cells. In sensory neurons, dcar-1::GFP was expressed in several amphid chemosensory neurons including the ASH neurons. dcar-1::GFP was also expressed in the ASI sensory neurons, the uv1 neuroendocrine cells, the uterine toroid cells, and the PVQ interneurons.

• Primary Identifier: Expr15164
• Pattern: Expression of the nlp-2p::gfp reporter transgene was restricted to one pair of head neurons and four uterine cells. Based on their location and sensory cilia morphology, we identified the head neurons as the olfactory AWA neurons. The uterus cells were identified as the neurosecretory uv1 cells.

0 Life Stages

1 Parents

Definition	Name	Synonym	Primary Identifier
monoucleate interfacial cell between the uterus and vulva (uv1-uv3), four sets total (L/R, A/P)	uterine-vulval cell	uv cell	WBbt:0006790