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Reporter gene fusion type not specified. |
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Expr4735
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Expression began at the comma stage and continued from the L1 to adult stages in body wall muscles. The tni-1::lacZ animals showed only weak expression in the body wall muscles. |
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Reporter gene fusion type not specified. |
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Expr4736
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Expression began at the comma stage and continued from the L1 to adult stages in body wall muscles. The tni-2::lacZ construct gave strong expression in all the body wall muscles. |
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Reporter gene fusion type not specified. |
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Expr4737
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Expression began at the comma stage and continued from the L1 to adult stages in body wall muscles. The tni-3::lacZ construct was expressed in all the body wall muscles from the L1 to adult stages. In the adult stage, tni-3::lacZ expression in body wall muscles became weaker with time although strong expression persisted in the head and vulval muscles. |
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Picture: Figure 5B. |
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Expr4986
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Consistent with the ubiquitous expression of sqt-3 throughout the life cycle, the R584 antiserum intensely stains the hypodermal cells and cuticles of all stages. Reactive antigens are first detected in wild-type comma-stage embryos within the hypodermal cells. The signal localizes perinuclearly, presumably in association with the secretory pathway. At the threefold stage, coincident with cuticle secretion, the signal becomes progressively extracellular: by the late pretzel stage, all antigen is detected in alignment with the annular ridges of the embryonic cuticle. |
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Expr4917
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Moderate bwm, mosaic, tail hyps, earlies bwm is comma with 1-2 cells +, by 3-fold fairly strong in many bwm; early gut at 12E, strong gut at 1.5 fold then fades to cytoplasmic and gone. |
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Expr4608
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Clear expression was shown in two of the hypodermal syncytia, hyp4, which surrounds the anterior part of the pharynx, and hyp7, which surrounds the mid-body region of the animal. The earliest detectable GFP expression can be observed in comma stage embryos, where there is abundant expression in the newly generated hyp4 nuclei and, albeit less frequently, in hyp7 nuclei. This expression pattern remains consistent throughout embryonic and larval development up until adulthood. There is faint expression in the hindgut, which most likely represents background expression of the reporter. |
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Expr4466
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Exclusively expressed in the pharyngeal muscle or marginal cells from the embryonic comma stage to adults. |
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Clone: pUL#JRH/AF08 |
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Expr7745
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Comma stage embryos show low levels of expression in virtually all cells but higher levels in outer cells. Late stage embryos show more localized expression in cells down sides and in the head. Post-embryonically expression is seen in several cells in the head, in the excretory cell, in cells of the developing and adult vulva, in rectal epithelia, & in seam cells. |
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Expr12443
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A GFP fusion gene with 7.7 kb of nsy-4 upstream sequence was expressed beginning at the comma stage of embryogenesis and continuing until the adult. At the 2-fold stage of embryogenesis, expression was prominent in the excretory cell and in anterior epidermal cells. At the first larval stage, the nsy-4 reporter transgene was expressed in the excretory cell, in epidermal cells in the head (some or all of hyp 1-6) and tail (some or all of hyp 8-11), and in the P neuro-epidermoblasts, both before and after their ventral migration. Weak nsy-4::GFP expression was detectable in a few neurons. In three appropriate mosaic animals identified at the L1/L2 stage, nsy-4::GFP was observed in the AWC cell body. Expression was not detectable in AWC in older animals. |
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Expr15555
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Using NeuroPAL, we confirmed sIs14542 expression in AVH, but not AVJ. We further corroborated hlh-34prom expression in AVH by observing that in the embryo, expression of otIs768 is first transiently observed in 4 cells, two of which show signs of cell death; later in embryogenesis, and then during all stages of postembryonic and adult development, expression becomes restricted to 2 cells. This is consistent with expression in the bilateral AVH neuron pair, since their two sisters cells are, unlike the sisters of the AVJ neuron pair, destined to die by apotosis (Sulston et al. 1983). |
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Picture: Fig 5G, 5H, 5I. |
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Expr9039
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The expression of nhr-206 GFP reporter transgenes starts during the comma stage of embryogenesis, and is initially seen in four unidentified cells localized in the head region. By the 2-fold stage, embryonic expression is observed in the pharynx with weaker expression in intestine. This expression pattern continues throughout all larval stages and in adults with pronounced anterior pharyngeal expression. The reporter genes were also strongly expressed in rectal gland cells, the anal sphincter, and in epidermal cells in the tail. Weaker expression was also observed in the vulva and spermatheca. In males, expression was visible in male specific neurons of the tail and rays. |
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This Expr_pattern is about CeTMIV, an isoform of tmy-1 transcription. To confirm the CeTMIV isoform expression pattern, corresponding tmy-1::gfp vectors were assayed. Similar GFP expressions induced in pharynx and intestines respectively. These results show that the CeTMIV isoform was expressed in the pharyngeal muscles and intestinal cells and establish that the primary promoter region was located within 853 bp upstream of the initial ATG. pretzel stage (author) = fully-elongated embryo (wjc). |
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Expr1679
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The constructs pTMZIV4349 and pTMZIV1957 induced beta-galactosidase expressions in both the pharyngeal muscles and intestinal cells with similar intensities. Specifically, pharyngeal expressions were observed in all the eight muscle cells (m1-m8), one marginal cell (mc), one epithelial cell (e1) and the four cells of the pharyngo-intestinal valve (PIV). The 20 intestinal cells, some of which are binucleated and localized alongside the intestine, posterior of pharynx and anterior of anus were all stained with intense staining occurring at the most posterior end. Intestinal staining was limited only to intestinal cells, although there were slight variations in position of nuclei and staining intensity. Expression was also detected in embryos between gastrulation and the comma stage. At this stage of development, the exact nuclei are difficult to identify, but the positions and topology suggest pharynx and intestines. By the pretzel stage, identification of the different pharyngeal muscles showing expression becomes possible. A similar expression was also observed in the pharynx of males, although the intestinal staining was more restricted to the posterior region. No expression was induced by the further deletion construct pTMZIV1219. In all cases, the most uniform and intense expression patterns were observed between L1 and L2 worms, and were completed by L2. From L3 to adult, there was a reduction in expression intensity. Both pharyngeal and intestinal expressions were evident within six hours after staining. |
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Feature: 'WBsf919537::pPD95.21' |
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Expr11811
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The distal enhancer activated reporter gene expression both inside and outside the pharynx. In larvae and adults, expression was observed in the m3, m4, m5, and m7 pharyngeal muscles as well as pharyngeal marginal cells, epithelial cells, and neurons. Expression was also observed outside the pharynx in the body wall muscles and the ventral nerve cord. Distal enhancer activity initiated in the pharynx at the bean stage of embryogenesis near the time that the endogenous ceh-22 gene is first expressed. |
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No GO_term assigned. |
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Expr2606
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Results from both GFP-expressing transgenes indicated that inx-6 is first detected during embryogenesis at the comma stage, in anterior cells that are likely to be the pharyngeal precursors. This pharynx-specific expression expands during the development of the pharynx to the end of embryogenesis, whereas inx-6 continues to be expressed in the corpus muscles and isthmus marginal cells of the pharynx throughout the larval and adult stages. |
The translational GFP fusion protein is expressed in a punctate expression pattern. |
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Reporter gene fusion type not specified. See Expr837, 838, 840 for Expr_pattern of the same locus. |
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Expr839
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Very strong beta-galactosidase expression in the pharynx from the comma stage of embryogenesis through late larval stages, and frequent expression was observed in muscle cells and marginal cells, while less frequent expression was observed in epithelial cells and the pharyngeal intestinal valve cells. Reporter expression was also observed outside the pharynx in a pattern similar to that of the endogenous PEB-1 protein. |
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Operon: CEOP1368 |
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Expr9452
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Animals bearing the transcriptional and translational reporters had similar GFP expression patterns. L1 animals carrying the translation reporter expressed GFP in many neurons, including CANs, DD-type motoneurons and ALMs. Expression in the nervous system began early in comma-stage embryos and peaked in intensity around the 3-fold stage of embryogenesis. Although neuronal expression was much fainter at later larval stages, it persisted in some head and tail neurons through adulthood. Non-neuronal cells that also expressed CRML-1::GFP included the migrating distal tip cells, the pharynx, some vulval epithelial cells, rectal epithelial cells and the excretory canal. |
Sub-cellular localization within the body wall muscle: Muscle cell membrane +/- Muscle arms |
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Expr9722
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Expression becomes detectable around the comma stage of embryogenesis and persists through adulthood. Expression in vulval precursor cells is strong and can first be seen in L3. PQN-47::GFP is expressed in seam cells, peaking at L2 and ceasing after the seam cells differentiate in late L4, concurrent with the appearance of alae. The intestine shows variably undetectable to low pqn-47 expression (always less than in the neurons) and gets dimmer as development progresses, especially after L3. The two bulbs of the pharynx, specifically pharyngeal muscle cells pm3-8 (not pm6), are variably bright. Overall expression levels are lower in adults than younger animals, with only some expression in head and tail neurons remaining. Head and nerve ring neurons, pharyngeal cells, ventral nerve cord cells, vulval precursor cells, seam (though interestingly not hyp7), as well as cells in the tail show the strongest pqn-47 expression. Muscle, intestine, the distal tip cells of the gonad, the spermatheca, and a large neuron that may be CAN that is essential for survival but of unknown function near the vulva (also bathed in pseudocoelom fluid, and next to the seam and canal cells), as well as a subset of the ciliated neurons of the head (amphid neurons ASI, ADL, ASK, or AWB) and tail including phasmid cilia PHA and PHB, also express pqn-47. We could not detect expression in the pharyngeal glands as reported for a different promoter pqn-47 fusion construct made as part of a high-throughput analysis of gene expression, although other tissues did show similar patterns. Promoter and translational reporters show pqn-47 expression in numerous somatic cells, including cells uniquely poised to mediate or transmit signal(s) involved in the regulation of molting, some of which have been implicated in molting. For example, many cells expressing PQN-47 have significant exposure to the pseudocoelom, and as such are candidates to transmit or detect endocrine signals; the H-shaped excretory cell and its ducts, which form extensive gap junctions with the hypodermis and lie against the pseudocoelom along the entire body of the worm (Nelson and Riddle, 1984), the head mesodermal cell (hmc) lies in the pseudocoelom up against the (excretory) gland cell and forms gap junctions with them and muscle, and the VPI cells at the juncture of the pharynx and intestine are bathed by the pseudocoelom, as well as the intestine itself. |
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Expr9837
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Nuclear-localized GFP was seen from the comma-stage embryo through to the mature adult in a variety of cell types. NB. Some variation was found between transgenic lines generated for the same reporter gene fusion clone. GFP was observed in many cells in embryos and from L1 to L3. Neurons in head, tail and ventral nerve cord expressed GFP, DVA particularly strongly in some larvae and adults. Muscle cell nuclei throughout the body contained GFP, with higher levels in the M lineage from the L1. In some L1s, L2s and L3s, GFP expression was observed in the P lineage although this was variable presumably due to mosaicism of the transgenic array. GFP was also seen in the developing vulva and reproductive muscles. Intestinal expression of GFP was present in L3s, L4s and adults, but inconsistently. |
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Expr12145
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Fluorescence in the transcriptional dpy-18prom::GFP reporter strain was first detected at the bean stage of embryogenesis in hypodermis and muscle, and gfp continued to be expressed in these tissues, as well as in motor neurons and in other unidentified neurons, during embryonic development. Postembryonically, dpy-18prom::GFP is expressed in a similar pattern and is more expansive than observed previously (Hill et al., 2000). In young larvae, dpy-18prom::gfp is expressed in the hypodermis, in muscle, in a few unidentified neurons in the head and tail, and in motor neurons. |
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No detailed description on expression patterns in adult stage. |
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Expr3847
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The GFP expression was more dynamic than that of EGL-20::GFP. GFP expression was first detected in the embryonic tail beginning at the comma stage. In the newly hatched larvae, CWN-1 was often expressed in four stripes of cells lining the dorsal and ventral posterior quadrants of the body, suggesting that most of the CWN-1-expressing cells were muscle cells. The expression spread anteriorly and became more restricted to the ventral side of the animal as development proceeded. |
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No detailed description on expression patterns in adult stage. |
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Expr3848
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The integrated array muIs49 contained a genomic egl-20 fragment with GFP fused in frame to sequences encoding the C terminus of EGL-20; larvae containing this transgene express GFP in a group of epidermal and muscle cells in the posterior near the anus. In embryos bearing this transgene, GFP fluorescence could not be detected the time when the HSNs migrate. muIs49 embryos were stained with an anti-GFP antiserum and these embryos expressed GFP in several cells of the developing tail beginning just before the comma stage and continuing throughout embryogenesis and larval development. |
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Expr10739
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glp-1(2in_493) is expressed in several neuronal precursor including the P blast cells in embryos at the comma stage. After hatching, glp-1(2in_493) expression was detectable in various neurons throughout all larval stages. At the L1-L2 stages, GFP fluorescence was evident in the ventral nerve cord. During the L3-L4 stages, glp-1(2in_493) expression was present in most, if not all, ventral nerve cord neurons. In addition, several neurons in the head and tail regions expressed glp-1(2in_493). For example, most cells of the pharyngeal nerve ring, including certain amphid neurons such as ASK, ADL, ASI and ASH, displayed glp-1(2in_493) expression. Some neuronal cells from the tail, including the phasmid neuron PHB, were also glowing. |
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Expr13310
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Expression of the established daf-7 promoter-GFP transcriptional reporter ksIs2 was detected in multiple developing neurons in comma-stage embryos, as well as in several neurons in 3-fold embryos. Importantly, accumulation of secreted mCherry::DAF-7 protein was detected in the extraembryonic fluid at the same embryonic stages as the SMAD::GFP fluorescence, suggesting that DAF-7 activity may have a physiological role in the early embryos. We also detected pharyngeal expression and localization to coelomocytes in 3-fold embryos. |
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This information was extracted from published material (Archana Sharma-Oates, Andrew Mounsey and Ian A. Hope). |
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Expr716
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Expression observed in most or all neurons at high levels as well as in some hypodermal and muscle cells. In most neurons, sax-3::GFP expression is transient, peaking during the period of axon outgrowth. Highest level of expression is observed in the embryo, particularly during the initial period of axon outgrowth at 350-400 min (comma-stage). At comma-stage high level of expression is observed in anterior embryo, including most developing neurons of the nerve ring and a swath of ventral cells that includes the developing motor neurons of the ventral nerve cord and posterior neurons such as PVQ. Lower level of expression is observed in the epidermal cells. 200-400 - cell stage; 200-300 min of development: Expression seen in all epidermal cells at low level. 3-fold stage; >500 min of development: Expression seen in muscles that extend from nerve ring to the anterior tip of the head. L1: Expression in motor neurons in the head including RMD, RMG, SMD, SIA and SIB neurons; projection interneurons in the head and tail, including AVA, AVB, PVC, AVD, PVQ and ALA neurons; and the sensory OLQ neuron. This neuronal expression diminishes throughout postembryonic development. During L1, expression persists in the head muscles and appears in muscles along the body wall, with ventral muscles expressing more strongly than posterior muscles. This expression continues until the adult stage. Epidermal expression was rarely seen in larval stages. L2: Expression observed in HSN motor neurons. HSN motor neurons extends a growth cone (axon from their lateral cell bodies) towards the ventral midline and expression is at its highest at this point. L3: Expression observed in the axon reaching the ventral nerve cord. L4: Expression continues in HSN, when HSN axon grows anteriorly to the head. Adult: Expression decreases in HSN at this stage after the completion of HSN axon outgrowth. In addition expression observed in motor neurons, interneurons and sensory neurons listed above also postembryonic ventral cord motorneurons, some interneurons from the tail and head, body wall and vulval muscles. |
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Feature : "ceh-22.pe39_pe41" |
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Expr11279
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Both pe39 and pe41 enhanced expression specifically in pharyngeal muscles. Transgenic lines bearing pe39:: pes-10::lacZ and pe41::pes-10::lacZ reporters exhibited robust reporter gene expression in the m3, m4, m5, and m7 pharyngeal muscles, cells that express the endogenous ceh-22 gene. In addition,the pe41::pes-10::lacZ was also expressed in one non muscle cell in the pharynx (provisionally identified as a g1 gland), the pharyngeal-intestinal valve cells, and a pair of neurons outside the pharynx. The onset of pharyngeal-galactosidase expression was as early as the comma stage of embryogenesis, and expression persisted in the pharyngeal muscles through the remainder of embryogenesis and larval development. Both the spatial and temporal specificity of the pe39 enhancer in particular was nearly indistinguishable from that of the full-length proximal enhancer. |
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Temporal description |
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Expr9298
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Expression of GFP was initially detected in embryos at the comma to 1.5-fold stages (310-350 min after first cell division) in the neurons, the intestine, and the body wall muscle. In older embryos, expression of GFP is gradually diminished in the body wall muscle, while it persisted in the neurons and intestine. In adult worms, expression of GFP was detected in the intestine, the spermatheca, and some of the head neurons. |
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Expr3038
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For din-1L, 8 kb of the din-1 upstream region was placed before gfp followed by din-1LB cDNA. Extrachromosomal arrays dhEx191 and dhEx194 were expressed in the nuclei of most cells from embryo to adult, as well as in dauer larvae. din-1L expressed more strongly in embryo and L1 larvae than din-1S, consistent with an earlier role. din-1S::gfp localized to the nuclei of most cell types. Detected in hypodermis, seam, intestine, and somatic gonad including the distal tip cells, din-1S was also expressed in neurons, vulval precursors, body wall muscle, and pharynx, all tissues with heterochronic phenotypes or remodeled during dauer. Expression was first detected in a few nuclei by the comma stage of embryogenesis. By hatch, din-1S was widely expressed, albeit weakly. Overall expression in most tissues was detected at various levels into adult and in dauer larvae. Interestingly, in late L1 and L2 larvae din-1S was often (75%, n = 16) highly expressed in the XXX cells, specialized neuroendocrine cells proposed to be a site of synthesis for the daf-9-produced hormone. |
Expressed in nuclei. |
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Expr9417
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strong pan neuronal; spermatheca; faint vulval mu; rare and faint bwm in adult; neuronal starts at bean with strong at 3-fold. |
Sub-cellular localization within the body wall muscle: Sarcoplasmic reticulum (SR)-like |
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Reporter gene fusion type not specified. |
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Expr1059
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Wild-type animals transformed with this construct show GFP fluorescence in most, if not all, neurons. Fluorescence first appears in comma stage embryos and persists through adulthood. In addition to neurons, expression was seen in the pharynx, in coelomocytes, and in the distal tip cell. rpm-1 is not to be expressed in body wall muscle, hypodermis, or intestine. |
The GFP signal in pSAM3 transformants is weak. Expression of the pSAM3 rescuing construct indicates that RPM-1 is most abundant in axons of the nerve ring neuropil. Clearly, RPM-1 is not confined to the nucleus. |
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Strain UL2626, UL2627 |
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Expr13606
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Larval and adult expression in the posterior intestine, body neurons, tail hypodermis, pseudocoelom and an unidentified structure around anus. Occasional spermathecae and excretory gland expression. Widespread expression in comma stage embryos. No intestinal, neuronal, hypodermal, excretory cell or pseudocoelom expression seen previously. No vulval expression seen in the current study. Larval and adult expression in the posterior intestine, body neurons, tail hypodermis, pseudocoelom and an unidentified structure around anus. Occasional spermathecae and excretory gland expression. Widespread expression in comma stage embryos. |
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