WormMine: AnatomyTerm WBbt:0004088

• Definition: Neuron, lateral process adjacent to excretory canal
• Name: PVDR
• Primary Identifier: WBbt:0004088
• Synonym: lineage name: V5R.paapa

1 Children

Definition	Name	Synonym	Primary Identifier
nucleus of pedigree V5R.paapa	V5R.paapa nucleus		WBbt:0002626

0 Expression Clusters

148 Expression Patterns

• Primary Identifier: Expr4345
• Pattern: GFP expression is nearly ubiquitous in the early embryo. At early comma stage expression becomes intensely focused in the anterior of the embryo. Strong expression is observed from the pharynx and some unidentified head neurons beginning around the 1.5-fold stage. After hatching, GFP expression is present in the ALM and PLM neurons. Beginning in late L1 stages expression comes on in the PVD neurons and some time later in the AVM. No significant expression of GFP was observed in ventral cord commissural motoneurons.

• Primary Identifier: Expr4648
• Pattern: Transgenic lines generated with the full-length protein fusion construct (for example, ljEx114) expressed TRPA-1:: GFP in the same cells as ljEx107 (see Expr4646), but with some additional cells, including the majority of amphid sensory neurons (for example, ASH, AWA, AWB, ASI and ASK) and the phasmid neurons PHA and PHB. The full-length TRPA-1:: GFP fusion was also expressed in the PVD and PDE in the postdeirid sensilla and the sensory neurons OLQ and IL1. Other neurons in the head and ventral nerve cord also expressed TRPA-1:: GFP.
• Subcellular Localization: The fusion protein was observed at the cilia of sensory neurons, as well as at the cell body.

• Primary Identifier: Expr16352
• Pattern: We confirmed previous reports that the asic-1 promoter drives expression in the ADE, CEP, PVQ, PDE and PVD neurons (De Stasio et al., 2018; Husson et al., 2012; Voglis & Tavernarakis, 2008) and also observed expression in FLP and ventral cord neurons.

• Primary Identifier: Expr15649

• Primary Identifier: Expr12224
• Pattern: Y55F3AM.3 is expressed mostly or exclusively in neurons including the PVD.

• Primary Identifier: Expr12231
• Subcellular Localization: Y55F3AM.3 translation fusion to GFP shows clear nuclear expression.

• Primary Identifier: Expr13955
• Pattern: dma-1, bicd-1, and flp-4 were expressed in FLP and PVD neurons.

• Primary Identifier: Expr15284
• Pattern: We observed prominent dma-1p::dma-1::gfp expression in the IL2 dendrites during dauer. Expression of dma- 1p::dma-1::gfp was not detectable in adult IL2s. dma-1p::dma-1::gfp translational reporter is prominently expressed in the FLPs and PVDs during dauer. We observed prominent dma-1p::dma-1::gfp expression in the IL2 dendrites during dauer. Expression of dma- 1p::dma-1::gfp was not detectable in adult IL2s.

• Primary Identifier: Expr9910
• Pattern: While DMA-1::GFP is dominantly expressed in PVD and FLP, especially by the adult stage, DMA-1::GFP expression in additional head and ventral cord neurons was detected during larval and early adult stages. DMA-1 expression was detected in the PVD cell body shortly after PVD are born (L2 larval stage) before any dendrite outgrowth or branching has occurred. Weaker DMA-1::GFP expression can be seen in ventral cord motorneurons and in the sub-lateral cords.Expression was variable from animal to animal, seen in both commissural and non-commissural motorneurons, and was mostly undetectable by the early adult stage. In the head, expression of DMA-1::GFP was detected during development in additional head neurons besides FLP. Of these neurons, the strongest expression was detected in neurons that run around the nerve ring and extend processes along the sub-lateral cords (likely some combinationof the SIA, SIB, SMB and/or SMD classes of neurons). A number of additional head neurons displayed inconsistent DMA-1::GFP expression from animal to animal. Again, expression in these additional neurons was transient and mostly undetectable by the early adult stage. d, Strong expression DMA-1::GFP was detected in vulva cells during the L3-L4 larval stages.
• Subcellular Localization: Bright DMA-1::GFP localization can be seen in intracellular membrane structures that are likely to be golgi/ER. The plasma membrane is also clearly visible, indicating that DMA-1::GFP is a cell-surface protein.

• Primary Identifier: Expr12470
• Subcellular Localization: DMA- 1::GFP localized in the dendritic membranes, and in some intracellular vesicles in wild-type animals. DMA-1::GFP containing vesicles mainly localized to the primary dendrites.

• Primary Identifier: Expr15558

• Primary Identifier: Expr15567

• Primary Identifier: Expr15571

• Primary Identifier: Expr15572

• Primary Identifier: Expr15573

• Primary Identifier: Expr15579

• Primary Identifier: Expr15586

• Primary Identifier: Expr15651

• Primary Identifier: Expr15652

• Primary Identifier: Expr15589

• Primary Identifier: Expr15591

• Primary Identifier: Expr15598

• Primary Identifier: Expr15604

• Primary Identifier: Expr14590
• Pattern: Embryonic expression of exc-7 was first observed at the bean stage. By reverse lineaging with use of SIMI-Biocell software, we confirm the identity of one of the expressing cells at this stage as the excretory canal cell. In L1 animals, broad expression in the head, ventral nerve cord (VNC), and tail was observed. In young adults, expression is notably observed in vulva cells. In the nervous system specifically, expression is observed in many neurons throughout the body, but unlike Drosophila Elav, exc-7::gfp it is not panneuronally expressed. We confirmed previously reported expression in cholinergic VNC MNs, but absence of GABAergic VNC MNs, consistent with previous reports (Fujita et al., 1999; Loria et al., 2003) and consistent with exc-7 functioning in cholinergic, but not GABAergic neurons to control alternative splicing (Norris et al., 2014). exc-7::gfp is also expressed in some non-neuronal cell types, including muscle and hypodermis, but not in the gut. A previous report showed that exc-7 is only transiently and weakly expressed in the excretory cell, which, based on exc-7's excretory mutant phenotype, has puzzled researchers (Fujita et al., 2003). We find that the gfp tagged exc-7 locus is strongly and continuously expressed in the excretory canal cell.

• Primary Identifier: Expr15608

• Primary Identifier: Expr11375
• Pattern: eat-4 is expressed in 78 of the 302 neurons of the adult hermaphrodite, which fall into 38 neuron classes (out of a total of 118 anatomically defined neuron classes in the hermaphrodite). Most of these neurons are either sensory- or interneurons. Only two motorneurons utilize glutamate; both are located in the pharynx.

• Primary Identifier: Expr15611

• Primary Identifier: Expr15997
• Reporter Gene: We used CRISPR/ Cas9 engineering to insert a tagRFP fluorescent protein at the N-terminus of CATP-8.
• Pattern: We found these animals to show widespread, if not ubiquitous expression of CATP-8, including in the epidermis, muscle, pharynx, intestine, and the major neuronal ganglia. Of note, we detected expression in both neurons that were phenotypically affected in catp-8 mutants as well as neurons that were not visibly affected. For example, clear expression of the endogenous tagRFP::CATP-8 reporter was seen in HSN, PVM, and PVD neurons, but also in ALM neurons and others. We found similar expression patterns in transgenic animals expressing GFP under control of the catp-8 5' region immediately upstream of the ATG start codon, where we also saw widespread expression from embryonic stages onwards, including in muscle, intestine, pharynx and other tissues. Collectively, our studies show that catp-8 is widely, if not ubiquitously, expressed albeit at different levels.

• Primary Identifier: Expr9874
• Remark: Picture: Figure 3.
• Subcellular Localization: EXL-1::GFP but not EXC-4::DsRed2 is targeted to muscle dense bodies (focal adhesion-like structures) and muscle Golgi membranes, the plasma membrane of muscle arms, and the Golgi apparatus in the neurons PVD and CAN. EXL-1::GFP targets multiple subcellular structures in coloemocytes cells, including lysosomes and the endoplasmic reticulum. EXC-4::DsRed2 and EXL-1::GFP are co-expressed in the intestine and in some neurons. In the intestine, EXC-4::DsRed2 and EXL-1::GFP co-localize to the intestinal luminal membrane and to lysosomes. Expression of EXC-4 and EXL-1 was observed in distinct but overlapping sets of neurons.

• Primary Identifier: Expr14627
• Pattern: sax-3 was expressed in the muscles and many neurons, including ALA and PVD.

0 Life Stages

3 Parents

Definition	Name	Synonym	Primary Identifier
post-embryonic cell of pedigree V5R.paap	V5R.paap		WBbt:0007466
Neuron class of two interneurons with cell bodies situated laterally in the posterior body.	PVD		WBbt:0006831
posterior lateral ganglion, right side.	posterior lateral right ganglion		WBbt:0005463