WormMine: ExpressionPattern Expr9598

• Pattern: The expression of CEH-23::GFP was restricted to a handful of neurons and the intestine of the worm. Although the neurons in which CEH-23 is expressed have not been identified, the observed neuronal expression of the CEH-23::GFP construct resembles that of a previously published GFP construct fused to a partial CEH-23 (gmIs18[ceh-23::gfp]), which was reported to express in the pair of CAN neurons in the central body and 12 sets of sensory neurons in the head (10 pairs) and the tail (2 pairs). While the neuronal expression of CEH-23 was found in all the transgenic worms, the intestinal expression was only detected in ,50% of the transgenic worms with a stronger expression in the posterior intestine. The expression of CEH-23::GFP was restricted to a handful of neurons and the intestine of the worm. Although the neurons in which CEH-23 is expressed have not been identified, the observed neuronal expression of the CEH-23::GFP construct resembles that of a previously published GFP construct fused to a partial CEH-23 (gmIs18[ceh-23::gfp]), which was reported to express in the pair of CAN neurons in the central body and 12 sets of sensory neurons in the head (10 pairs) and the tail (2 pairs). While the neuronal expression of CEH-23 was found in all the transgenic worms, the intestinal expression was only detected in 50% of the transgenic worms with a stronger expression in the posterior intestine.
• Primary Identifier: Expr9598
• Reporter Gene: [CEH-23::GFP] translational fusion. A 10.3 Kbp genomic fragment containing the 7.3 Kbp sequence upstream of the ceh-23 coding region, the 1.8 Kbp ceh-23 coding region, and the 1.1 Kbp sequence downstream of the ceh-23 coding region was amplified from genomic DNA by PCR. A GFP coding sequence without stop codon was amplified by PCR from the plasmid pPD95-81. The amplified GFP sequence was then fused in frame at the N-terminal of the ceh-23 coding region into the 10.3 Kbp genomic fragment described above. --precise ends.
• Subcellular Localization: Enriched nuclear localization of CEH-23 was detected in both the neurons and the intestine.

2 Anatomy Terms

Definition	Name	Synonym	Primary Identifier
A chain of very large cuboidal cells forming a wide central lumen in which food arrives from the posterior pharynx, is digested, and from which waste products proceed to the rectum. Intestinal rings form in groups of two and four cells surrounding the common lumen; thus the epithelium is only one cell deep at any point, with neighboring cells firmly secured to their neighbors by apical adherens junctions. These cells have very large nuclei and many large vacuoles, yolk granules, and other inclusions; the latter increase in number and electron density as the animal ages.	intestine	gut	WBbt:0005772
Major cell type of nervous tissue, specialized for transmission of information in the form of patterns of impulses.	neuron	neurone	WBbt:0003679

1 Genes

WormBase Gene ID	Gene Name	Sequence Name	Organism
WBGene00000446	ceh-23	ZK652.5	Caenorhabditis elegans

0 Life Stages