WormMine

WS294

Intermine data mining platform for C. elegans and related nematodes

Expression Pattern :

Pattern  The CDR-4-eGFP fusion protein was expressed predominately in the intestinal cells of the nematodes. Primary Identifier  Expr7891
Remark  Picture: Figure 6. Subcellular Localization  It was concentrated in small punctate structures within these cells. The size and distribution of these structures were similar to those of lysosomes and of CDR-1-eGFP. It has been shown that when a nonfusion form of eGFP is expressed in intestinal cells, it accumulates in the cytoplasm, not in vesicles. The transgenic nematodes were fed rhodamine B isothiocyanate (RITC)-labeled dextran, which labels intestinal cell lysosomes, to confirm that CDR-4 was targeted to the lysosomes. In the double-labeling experiment, both eGFP and rhodamine colocalized to the same structures, confirming that CDR-4 is targeted to intestinal cell lysosomes.
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5 Anatomy Terms

Definition Name Synonym Primary Identifier
A chain of very large cuboidal cells forming a wide central lumen in which food arrives from the posterior pharynx, is digested, and from which waste products proceed to the rectum. Intestinal rings form in groups of two and four cells surrounding the common lumen; thus the epithelium is only one cell deep at any point, with neighboring cells firmly secured to their neighbors by apical adherens junctions. These cells have very large nuclei and many large vacuoles, yolk granules, and other inclusions; the latter increase in number and electron density as the animal ages. intestine gut WBbt:0005772
the feeding organ, a neuro-muscular pump in the head of the animal, used to ingest food, bacteria suspended in liquid, filter them out, grind them up and transport posteriorly into the instestine. pharynx esophagus WBbt:0003681
seventh pharyngeal muscle cell layer pm7 m7 WBbt:0003721
fifth pharyngeal muscle cell layer pm5 M5 WBbt:0003737
sixth pharyngeal muscle cell layer pm6 m6 WBbt:0003724

1 Genes

WormBase Gene ID Gene Name Sequence Name Organism
WBGene00010470 cdr-4 K01D12.11 Caenorhabditis elegans

0 Life Stages