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Clone: pUL#JRH/AF1 |
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Expr7709
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Generalised expression is seen, late embryo to adult, although expression is stronger in the intestine and posterior pharynx. The generalised expression definitely includes hypodermis, nerve cells, muscle cells and the excretory cell, as the lines are heavily mosaic and so expression in these cell types could be recognised. Expression in the hypodermis includes the tail spike. |
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Picture: Figure 4A to G. |
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Expr8076
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Expression was found in the excretory canal cell, tail spike, uterine seam cell, distal tip cells, intestine, ALM and PLN neurons, and nerve ring. For male worms, although ray development is affected, no GFP was detected in the mature rays, although GFP is weakly expressed in the tail spike in larval stages. |
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Picture: Fig. 4H. |
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Expr8077
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The same expression pattern as Expr8076. |
Expression was seen throughout the cytoplasm. |
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A total of 12 independent transgenic lines were generated via bombardment and all of them showed the similar expression patterns. Picture: Fig 1, Fig S1, Fig S2. |
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Expr9123
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Examination of larvae and adults showed that the reporter expression remained confined to the posterior of the animal with the exception of weak expression in more anterior intestinal cells. Signal appears to increase through the L2 stage, after which it decreases with only minimal levels detectable in the tails of adults in hermaphrodites. By contrast in males the adult tail shows high levels of expression. Stably integrated lines with a 2.26 kb fragment upstream of the mir-57 mature sequence fused with a fluorescent reporter mCherry showed expression in the posterior cells of the embryo in a variety of tissues. Automated analysis of 3D time-lapse movies followed by manual editing revealed that the reporter was expressed in a bilaterally symmetric pattern in the posterior daughters of sublineages of AB and C founder cells, beginning at about the 200-cell stage. The cells from these sublineages lie in the posterior part of the embryo only and represent a wide variety of cell types, including tail seam cells, the hypodermal cells hyp10 and hyp11, the cells producing the tail spike, rectal cells, the P11/12 cells and even body wall muscle cells. Inspection of the movies beyond the comma stage also showed expression in the intestinal cells after elongation. |
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Expr10745
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dre-1 promoter::GFP is robustly expressed in the tail- spike cell but not in the surrounding hyp10 hypodermal cell that forms the tail spike. |
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Expr15860
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We found that blmp-1 transcription is detected in the tail-spike cell as early as the mKatePH reporter (1.5-fold stage). blmp-1 transcription continues until the tail-spike cell dies with a characteristic rounded refractile morphology at the 3.7-fold stage. |
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Expr15861
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We found that, like the transcriptional reporter, endogenous BLMP-1::GFP is detected in the tail-spike cell from the 1.5-fold stage until the cell dies. |
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