WormMine: ExpressionPattern Expr9123

• Pattern: Examination of larvae and adults showed that the reporter expression remained confined to the posterior of the animal with the exception of weak expression in more anterior intestinal cells. Signal appears to increase through the L2 stage, after which it decreases with only minimal levels detectable in the tails of adults in hermaphrodites. By contrast in males the adult tail shows high levels of expression. Stably integrated lines with a 2.26 kb fragment upstream of the mir-57 mature sequence fused with a fluorescent reporter mCherry showed expression in the posterior cells of the embryo in a variety of tissues. Automated analysis of 3D time-lapse movies followed by manual editing revealed that the reporter was expressed in a bilaterally symmetric pattern in the posterior daughters of sublineages of AB and C founder cells, beginning at about the 200-cell stage. The cells from these sublineages lie in the posterior part of the embryo only and represent a wide variety of cell types, including tail seam cells, the hypodermal cells hyp10 and hyp11, the cells producing the tail spike, rectal cells, the P11/12 cells and even body wall muscle cells. Inspection of the movies beyond the comma stage also showed expression in the intestinal cells after elongation.
• Primary Identifier: Expr9123
• Remark: A total of 12 independent transgenic lines were generated via bombardment and all of them showed the similar expression patterns. Picture: Fig 1, Fig S1, Fig S2.

9 Anatomy Terms

Definition	Name	Synonym	Primary Identifier
A chain of very large cuboidal cells forming a wide central lumen in which food arrives from the posterior pharynx, is digested, and from which waste products proceed to the rectum. Intestinal rings form in groups of two and four cells surrounding the common lumen; thus the epithelium is only one cell deep at any point, with neighboring cells firmly secured to their neighbors by apical adherens junctions. These cells have very large nuclei and many large vacuoles, yolk granules, and other inclusions; the latter increase in number and electron density as the animal ages.	intestine	gut	WBbt:0005772
Longitudinal bands of muscle cells surrounding animal body, with one band running in each quadrant of the body, regulated contraction and relaxation of these muscles cause locomotion.	body wall musculature	body muscle	WBbt:0005813
a group of hypodermal cells that lie along the apical midline of the hypodermis, at the extreme left and right sides between nose and tail	seam cell	lateral hypodermis	WBbt:0005753
Tail ventral hypodermis	hyp10	lineage name: ABplppppppp	WBbt:0004378
Tail dorsal hypodermis	hyp11	lineage name: Cpappv	WBbt:0004377
Postembryonic blast cells for ventral cord motorneurons, ventral hypodermis, vulva, male preanal ganglion; ventral hypodermis in L1	P11	lineage name: ABplapappa	WBbt:0004410
Postembryonic blast cells for ventral cord motorneurons, ventral hypodermis, vulva, male preanal ganglion; ventral hypodermis in L1	P12	lineage name: ABprapappa	WBbt:0004409
epithelium connecting intestine and anus.	rectal epithelium		WBbt:0005800
An acellular thin cuticle whip formed at the very end of the tail during embryogenesis.	tail spike		WBbt:0006979

1 Genes

WormBase Gene ID	Gene Name	Sequence Name	Organism
WBGene00003285	mir-57	T09A5.13	Caenorhabditis elegans

6 Life Stages

Remark	Definition	Other Name	Public Name	Primary Identifier
	The second stage larva. At 25 Centigrade, it ranges 25.5-32.5 hours after fertilization, 11.5-18.5 hours after hatch.		L2 larva Ce	WBls:0000027
	The fourth stage larva. At 25 Centigrade, it ranges 40-49.5 hours after fertilization, 26-35.5 hours after hatch.		L4 larva Ce	WBls:0000038
	The first stage larva. At 25 Centigrade, it ranges 14-25.5 hours after fertilization, 0-11.5 hours after hatch.		L1 larva Ce	WBls:0000024
	The stage that begins when a C.elegans individual is fully-developed and has reached maturity.		adult Ce	WBls:0000041
	The third stage larva. At 25 Centigrade, it ranges 32.5-40 hours after fertilization, 18.5-26 hours after hatch.		L3 larva Ce	WBls:0000035
	The whole period of embryogenesis in the nematode Caenorhabditis elegans, from the formation of an egg until hatching.		embryo Ce	WBls:0000003