WormMine: AnatomyTerm WBbt:0004849

• Definition: Vulval muscle 1, of lineage M.vrpaapaa.
• Name: M.vrpaapaa
• Primary Identifier: WBbt:0004849
• Synonym: lineage name: M.vrpaapaa

1 Children

Definition	Name	Synonym	Primary Identifier
nucleus of pedigree M.vrpaapaa	M.vrpaapaa nucleus		WBbt:0002237

3 Expression Clusters

Regulated By Treatment	Description	Algorithm	Primary Identifier
	Single-cell RNA-Seq cell group 7_1 expressed in muscle.	scVI 0.6.0	WBPaper00065841:7_1
	Single-cell RNA-Seq cell group 143_0 expressed in muscle.	scVI 0.6.0	WBPaper00065841:143_0
	Single-cell RNA-Seq cell group 7_0 expressed in muscle.	scVI 0.6.0	WBPaper00065841:7_0

18 Expression Patterns

• Primary Identifier: Expr2029
• Remark: Other strains-- UL963, UL982, UL984
• Pattern: Strong expression is seen predominantly in the muscles of the pharynx, the pharyngeo-intestinal valve, and in cells surrounding the rectum, which appear to be the anal depressor muscle and the intestino-rectal valve. Other expression is occasionally seen in the vulval muscle cells (vm1 and vm2), bodywall muscle cell nuclei, and cells within the tail spike. Expression is seen from late embryo to adulthood.

• Primary Identifier: Expr3912
• Pattern: Expressed in vm1, pharynx, tail neurons, gonadal sheath cells.

• Primary Identifier: Expr15
• Remark: life_stage summary : postembryonic
• Pattern: Expression is seen in 4 neural cells of the anterior ganglion, 8 more in the lateral/ventral ganglia, and 4-6 nuclei of the tail ganglia. Staining is also seen on sub-cellular particles in the vm1 muscles of the vulva. The neural pattern develops from L1 onwards; the vulval staining occurs L4 onwards.
• Subcellular Localization: Expressed in nuclei.

• Primary Identifier: Expr17
• Remark: No GO_term assigned.
• Pattern: Expression first becomes evident at the first larval stage, L1, when the head body-wall muscles and an unidentified cell(s) in the anal region harbour stain. The anal staining lasts only until L2/3, whilst the head muscles continue showing expression right through to adulthood. The expression pattern includes a further component in the vm1 cells associated with the vulva. These specialised muscle cells attach to the ventral hypodermis and the vulval labia, and are important in opening the vulva during egg-laying. Expression here is transient, seen only for a short time around the L4 to adult molt. All components of the expression pattern are non-nuclear localised. The nuclei of the vm1 vulval muscle stain up, as do body wall muscles in the head. Vulval staining can be seen L4-young adult, with the head muscles staining earlier al ~L1-L3.
• Subcellular Localization: Expression is non-nuclear localized.

• Primary Identifier: Expr1222
• Pattern: In adult animals, GFP signal was found in all body wall muscle cells, in the three pharyngeal cells pm5 and in the anal sphincter muscle. A weak expression was observed in four of the eight vulval muscle cells (vm1) whereas in males, GFP was expressed in diagonal and spicule muscles. GFP was expressed also in three pairs of cephalic sensory neurons located in anterior (two pairs) and dorsal (one pair) head ganglia, respectively. These neurons possessed endings in the labial region and were identified as the outer lateral labial cells (OLL) and the four sensory cephalic neurons CEP (the ventral CEP pair is ventral and anterior to the nerve ring, the dorsal CEP pair is posterior to the nerve ring). During development, GFP was detected in embryos at the 1-1/2-fold stage, in one muscle quadrant. For larval stages, an expression pattern similar to that in adults was observed, but in early L1, a strong signal was detected in the mesodermal M cell in the mid-part of the body.

• Primary Identifier: Expr1918
• Pattern: In the pAB::GFP fusion, expression was seen in some pioneering neurones of the nerve ring, beginning at the early comma stage. At the two-fold stage, expression was detected in some 10 neurones in the head that extend axons into the nerve ring, and in two neurones in the tail that extend processes anteriorly. This expression pattern was confirmed by immunohistochemistry with MAb 16-48-2. At the three-fold stage, expression was seen in all DA motoneurones and persisted while they pioneered the dorsal nerve chord. It was also seen in four to six neurones in each of the four head ganglia, including ALA and RID in the dorsal ganglion, and four of the six neurones of the terminal bulb, including M5. In the tail, two neurones in the pre-anal ganglion and six in the lumbar ganglion, including PVQL and PVQR, showed pAB::GFP expression. Additionally, a transient expression was seen in the four rows of bodywall muscle cells in the embryo. After hatching, in L1 larvae, the expression domain extended to amphid and phasmid socket cells, and subsequently in L2 larvae to all the newly born AS motoneurones. In hermaphrodite L3 larvae, expression was seen in the sex myoblasts subsequent to their anterior migration towards the position of the presumptive vulva, and in adult worms at a high level in the vulval muscles vm1 and vm2. In males, expression was seen in the diagonal and spicule retractor muscles.

• Primary Identifier: Expr1920
• Pattern: Expressed in nine DA, 11 AS, ALA, RID, two PDE, two HSN, M5, four vulval muscles 1, four vulval muscles 2, four uterine muscles 1, four uterine muscles 2, two intestine muscles, sphincter muscle, 15 diagonal muscles, two spicule retractors, bodywall muscles, two AM socket cells, two PH socket cells, two distal tip cells.

• Primary Identifier: Expr1786
• Pattern: Fluoresence detected in the ALA, GLR, and other neurons. Muscle expression was observed in the VMI, intestinal muscle cells, certain anterior body wall muscles and probably the anal sphinctor. GFP was also present in the seam, distal tip cells, celomocytes, a socket cell associated with a nonamphid neuron, as well as other as yet unidentified cells.

• Primary Identifier: Expr3910
• Pattern: Expressed in vm1, pharyngeal muscles, depressor.

• Primary Identifier: Expr3902
• Pattern: Expressed in depressor, sphincter, vm1, ?intestinal muscles, hypodermis.

• Primary Identifier: Expr3896
• Pattern: Expressed in intestinal muscles, vm1, pm8, head neurons, spermatheca and rectal epithelial cells.

• Primary Identifier: Expr3899
• Pattern: Expressed in head/tail neurons, ventral nerve cord, alae, gut cells, vm1 and vulval epithelial cells

• Primary Identifier: Expr3916
• Pattern: Expressed in vm1, 6 pairs of head neurons, gut cells and tail neurons.

• Primary Identifier: Expr123
• Remark: Other strain-- (180.4 -expression observed, died out). late embryo(author) = 3-fold embryo + fully-elongated embryo(curator).
• Pattern: This simple expression pattern expresses in the body wall muscle and in the vm1 vulval muscles. The staining of the bodywall muscles is strongest in the head and anterior of the worm. Expression begins in 3-fold embryos, and is seen throughout larval stages to adulthood. The vulval muscles express in L4 larvae and adults. Both components display mosaic expression.

• Primary Identifier: Expr446
• Remark: Other strain-- showed no observable expression.
• Pattern: Expression is seen in the vulval muscles (vm1 and vm2) and body-wall muscle of adult worms. In body-wall muscle cells expression is localised to thick filaments and dense bodies.
• Subcellular Localization: In body-wall muscle cells expression is localised to thick filaments and dense bodies.

• Primary Identifier: Expr3900
• Pattern: Expressed in head/tail neurons, ventral nerve cord, depressor, vm1.

• Primary Identifier: Expr1802
• Remark: Reporter gene fusion type not specified.
• Pattern: A stably integrated egl-15::gfp transgene is expressed in the four Type 1 vulval muscles (vm1s): one in each of the four egg-laying muscle quadrants.

• Primary Identifier: Expr3907
• Pattern: Possicle weak expression in vm1.

0 Life Stages

2 Parents

Definition	Name	Synonym	Primary Identifier
Set one type of vulval muscle.	vm1	vulval muscle, type 1	WBbt:0006917
	M.vrpaapa		WBbt:0008532