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Expr3815
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From this construct, unc-103 also expresses in the pharyngeal neurons (I2 and NSM), head neurons [IL1, IL2, OLL, URAD, ASH, AVD, AUA, and SIAV and OLQ, RIV, URYV, AIN, and AIA, PCA in the post-cloacal sensilla, and one of two ray neurons in rays 1, 2, 3, 4, 6, and 9. An unc-103::YFP translational fusion expressed from the 8.7 kb upstream region was also injected and it was observed that the anal depressor, spicule protractor, retractor, and other male sex muscles also expressed UNC-103; in the hermaphrodite, the vulva muscles also express the transgene. |
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Picture: Fig. 3C,D. |
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Expr8296
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Expressed in the SPC motor neurons, PCB and PCA postcloacal sensilla (p.c.s.) neurons, male spicule protractor muscles and anal depressor muscle, VD and DD ventral cord neurons, some tail and nerve ring neurons, and body wall muscles. In hermaphrodites, YFP expression is in the same set of cells, except those specific to males. |
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Expr9999
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A 2.8 kb promoter of cysl-1 drove GFP expression mainly in the nervous system of adult hermaphrodites. The cysl-1::GFP expression pattern was similar for the transcriptional and translational reporters. GFP was observed in subsets of pharyngeal neurons, amphid sensory neurons and tail neurons, starting from late embryonic stages and persisting into adults. We identified GFP-positive cells as the AVM sensory neuron, the BDU interneurons, and the pharyngeal I1 interneurons and M2 motor neurons, based on their characteristic processes and nuclear positions. GFP in body wall muscles, hypoderm, and intestine was present in larvae but only weakly detectable in adult animals. |
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Expr12313
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In these transgenic males, UNC-7 expression was found, in addition to the sex muscles (Expr12312), in several mating circuit neurons (SPC, PCA, PCB, HOA, and unidentified ray neurons). Similar to previous studies (Starich et al., 2009; Yeh et al., 2009; Kawano et al., 2011), this reporter also expresses in head ganglion neurons, several ventral chord neurons, and tail neurons of the hermaphrodite, in addition to few unreported pharyngeal neurons (I5, M2, I4, M3, and I1). |
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Expr10584
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The prig-6RIG-6::GFP construct drives expression of all RIG-6 isoforms in fusion with GFP, under their endogenous promoter. rig-6 expression is detected during embryogenesis and is maintained throughout adulthood. rig-6 is expressed in I1 and I3 pharyngeal interneurons, NSM motor neurons and other unidentified head neurons. RIG-6::GFP is also localized in the cell bodies and axons of different subtypes of VNC motorneurons, in the HSN and the CAN neurons. Neuronal expression of rig-6 also includes the ALM and PLM touch receptor neurons. Non-neuronal cells expressing rig-6 include somatic muscles and the hypodermis. We also detected rig-6 expression in the developing and adult EC. The prig-6RIG-6::GFP construct drives expression of all RIG-6 isoforms in fusion with GFP, under their endogenous promoter. rig-6 expression is detected during embryogenesis and is maintained throughout adulthood. rig-6 is expressed in I1 and I3 pharyngeal interneurons, NSM motor neurons and other unidentified head neurons. RIG-6::GFP is also localized in the cell bodies and axons of different subtypes of VNC motorneurons, in the HSN and the CAN neurons. Neuronal expression of rig-6 also includes the ALM and PLM touch receptor neurons. Non-neuronal cells expressing rig-6 include somatic muscles and the hypodermis. We also detected rig-6 expression in the developing and adult EC. |
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Expr12408
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With a construct including 8 kb upstream of the first exon and the entire unc-7 genomic region fused to YFP, UNC-7 expression was found, in addition to the sex muscles reported in Expr12407, in several mating circuit neurons (SPC, PCA, PCB, HOA, HOB, and unidentified ray neurons). Similar to previous studies (Starich et al., 2009; Yeh et al., 2009; Kawano et al., 2011), this reporter also expresses, in head ganglion neurons, several ventral chord neurons, and tail neurons of the hermaphrodite, in addition to few unreported pharyngeal neurons (I5, M2, I4, M3, and I1). |
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