WormMine: AnatomyTerm WBbt:0005791

• Name: intestinal lumen
• Primary Identifier: WBbt:0005791

0 Children

0 Expression Clusters

1 Expression Patterns

• Primary Identifier: Expr2601
• Remark: These patterns are specific because MH5 and polyclonal VAB-10A antibodies failed to detect a signal in vab-10(h1356) and vab-10Adeficient embryos. Likewise, VAB-10B antibodies could only detect a weak signal in the intestine and pharynx of mid-staged vab-10B(mc44) and vab-10(h1356) embryos. Although it cannot be excluded that VAB-10B antisera recognize a cross-reacting protein, VAB-10B is likely to be expressed in the pharynx and the intestine because surviving vab-10B(mc44) larvae had an abnormal intestine.
• Pattern: In embryos, VAB-10A and VAB-10B antibodies first detected a signal at the basal and apical plasma membranes of dorsal and ventral epidermal cells, soon after the onset of differentiation. As these cells became thinner during morphogenesis, staining appeared as four longitudinal rows and progressively evolved into circumferentially oriented bands located above muscle sarcomeres. VAB-10A was also detected basally and apically in the pharynx and along mechanosensory axons. VAB-10B was also present in the pharynx lumen, intestine lumen, nerve ring, and in body wall muscles and somatic gonad of larvae.
• Subcellular Localization: The circumferential bands formed by VAB-10A matched those formed by IFs at all stages. VAB-10A staining strictly colocalized with myotactin in adults, but only partially in young larvae. The parallel bands formed by VAB-10B are interspersed with those formed by VAB-10A and extend beyond the area of muscle epidermis contact. The VAB-10B bands corresponded to the regularly spaced shallow furrows that pattern the cuticle and separate annuli ridges. Immunoelectron microscopy confirmed that VAB-10A (but not VAB-10B) is restricted to FOs. VAB-10A was exclusively detected in the epidermis in areas dense in filaments, a characteristic of FOs. Using the VAB-10B K22 antibodies, gold particles were observed in the epidermis adjacent to body wall muscles and in sarcomeres. Although the number of particles is low, quantitative analysis indicates that staining is specific. Thus, VAB-10B appears to have a disperse distribution in the epidermis and muscles.

0 Life Stages

2 Parents

Definition	Name	Synonym	Primary Identifier
A chain of very large cuboidal cells forming a wide central lumen in which food arrives from the posterior pharynx, is digested, and from which waste products proceed to the rectum. Intestinal rings form in groups of two and four cells surrounding the common lumen; thus the epithelium is only one cell deep at any point, with neighboring cells firmly secured to their neighbors by apical adherens junctions. These cells have very large nuclei and many large vacuoles, yolk granules, and other inclusions; the latter increase in number and electron density as the animal ages.	intestine	gut	WBbt:0005772
Anatomical structures that exist outside of cells; non-cellular.	extracellular component		WBbt:0005732