WormMine: ExpressionPattern Expr2601

• Pattern: In embryos, VAB-10A and VAB-10B antibodies first detected a signal at the basal and apical plasma membranes of dorsal and ventral epidermal cells, soon after the onset of differentiation. As these cells became thinner during morphogenesis, staining appeared as four longitudinal rows and progressively evolved into circumferentially oriented bands located above muscle sarcomeres. VAB-10A was also detected basally and apically in the pharynx and along mechanosensory axons. VAB-10B was also present in the pharynx lumen, intestine lumen, nerve ring, and in body wall muscles and somatic gonad of larvae.
• Primary Identifier: Expr2601
• Remark: These patterns are specific because MH5 and polyclonal VAB-10A antibodies failed to detect a signal in vab-10(h1356) and vab-10Adeficient embryos. Likewise, VAB-10B antibodies could only detect a weak signal in the intestine and pharynx of mid-staged vab-10B(mc44) and vab-10(h1356) embryos. Although it cannot be excluded that VAB-10B antisera recognize a cross-reacting protein, VAB-10B is likely to be expressed in the pharynx and the intestine because surviving vab-10B(mc44) larvae had an abnormal intestine.
• Subcellular Localization: The circumferential bands formed by VAB-10A matched those formed by IFs at all stages. VAB-10A staining strictly colocalized with myotactin in adults, but only partially in young larvae. The parallel bands formed by VAB-10B are interspersed with those formed by VAB-10A and extend beyond the area of muscle epidermis contact. The VAB-10B bands corresponded to the regularly spaced shallow furrows that pattern the cuticle and separate annuli ridges. Immunoelectron microscopy confirmed that VAB-10A (but not VAB-10B) is restricted to FOs. VAB-10A was exclusively detected in the epidermis in areas dense in filaments, a characteristic of FOs. Using the VAB-10B K22 antibodies, gold particles were observed in the epidermis adjacent to body wall muscles and in sarcomeres. Although the number of particles is low, quantitative analysis indicates that staining is specific. Thus, VAB-10B appears to have a disperse distribution in the epidermis and muscles.

6 Anatomy Terms

Definition	Name	Synonym	Primary Identifier
Epidermal layer.	hypodermis	epidermis	WBbt:0005733
Longitudinal bands of muscle cells surrounding animal body, with one band running in each quadrant of the body, regulated contraction and relaxation of these muscles cause locomotion.	body wall musculature	body muscle	WBbt:0005813
the most extensive region of neuropil in the animal, consists of a large toroidal bundle of processes.	nerve ring	circumpharyngeal nerve ring	WBbt:0006749
the space in the center of pharynx which allows the passage of food.	pharyngeal lumen		WBbt:0005790
organ producing either sperm or ova.	gonad		WBbt:0005175
	intestinal lumen		WBbt:0005791

1 Genes

WormBase Gene ID	Gene Name	Sequence Name	Organism
WBGene00006876	vab-10	ZK1151.1	Caenorhabditis elegans

0 Life Stages