WormMine: ExpressionPattern Expr1117

• Pattern: HSP16-2 is ubiquitously expressed throughout most somatic tissues of larvae after heat-shock, and the intensity gradually decreased with age. HSP16 labelling is prominent in spermathecae and in the vulval region in L4 and adult hermaphrodites. The spermathecal labelling seems to consist of cytoplasmic localization of HSP16-2 in sperm, and perhaps also in the spermathecal cage cells. The prominent localization of HSP16 to intestinal cells was previously noted in transgenic animals expressing a lacZHSP16 gene fusion. Vulval expression of HSP16 was also seen in transgenic reporter experiments. In males, HSP16-2 was distributed more generally, though it was slightly concentrated in the lower testis in the region corresponding to spermatids, and in tail structures. The latter expression pattern was also seen with transgenic reporter constructs.
• Primary Identifier: Expr1117
• Remark: Reporter gene fusion type not specified. Similar patterns of HSP16 distribution were observed with two other polyclonal antibodies, one with greater specificity for HSP16-41 and another which cross-reacts with all four stress-inducible HSP16s. Thus the staining pattern described here reflects the distribution of the HSP16 family in general. Non-stressed animals showed little or no detectable signal.
• Subcellular Localization: Counterstaining with DAPI revealed HSP16 localized in discrete regions within the cytoplasm of intestinal cells, characterized by their large nuclei. Concentrations of HSP16-2 occur along the luminal border of these cells.

5 Anatomy Terms

Definition	Name	Synonym	Primary Identifier
A chain of very large cuboidal cells forming a wide central lumen in which food arrives from the posterior pharynx, is digested, and from which waste products proceed to the rectum. Intestinal rings form in groups of two and four cells surrounding the common lumen; thus the epithelium is only one cell deep at any point, with neighboring cells firmly secured to their neighbors by apical adherens junctions. These cells have very large nuclei and many large vacuoles, yolk granules, and other inclusions; the latter increase in number and electron density as the animal ages.	intestine	gut	WBbt:0005772
female genital.	vulva		WBbt:0006748
an accordion-like tube that contains sperm and is the site of oocyte fertilization.	spermatheca		WBbt:0005319
Male haploid germ cell.	sperm	spermatozoa	WBbt:0006798
An immature sperm cell which undergoes morphogenesis to form mature, amoeboid sperm.	spermatid		WBbt:0006800

1 Genes

WormBase Gene ID	Gene Name	Sequence Name	Organism
WBGene00002016	hsp-16.2	Y46H3A.3	Caenorhabditis elegans

2 Life Stages

Remark	Definition	Other Name	Public Name	Primary Identifier
	The stage that begins when a C.elegans individual is fully-developed and has reached maturity.		adult Ce	WBls:0000041
	A developmental life stage of the nematode Caenorhabditis elegans that occurs from egg hatching until adulthood.		larva Ce	WBls:0000023