WormMine: ExpressionPattern Expr3839

• Pattern: Using a gfp-tagged smk-1 cDNA construct under the control of the endogenous smk-1 promoter to create a stable transgenic line, strong nuclear localization of SMK-1-GFP was observed in intestinal cells. GFP fluorescence was also detected in the nuclei of several hypodermal cells and in many neurons in the head and tail.
• Primary Identifier: Expr3839
• Remark: The GFP signal was reduced upon treatment with smk-1 RNAi, and Western blot analysis showed that SMK-1 protein levels were reduced upon smk-1 RNAi treatment.
• Subcellular Localization: Expressed in nuclei.

4 Anatomy Terms

Definition	Name	Synonym	Primary Identifier
A chain of very large cuboidal cells forming a wide central lumen in which food arrives from the posterior pharynx, is digested, and from which waste products proceed to the rectum. Intestinal rings form in groups of two and four cells surrounding the common lumen; thus the epithelium is only one cell deep at any point, with neighboring cells firmly secured to their neighbors by apical adherens junctions. These cells have very large nuclei and many large vacuoles, yolk granules, and other inclusions; the latter increase in number and electron density as the animal ages.	intestine	gut	WBbt:0005772
Epidermal layer.	hypodermis	epidermis	WBbt:0005733
neuron with its cell body situated in the head, excluding the pharynx.	head neuron		WBbt:0006751
neuron with its cell body situated in the tail, posterior to rectum.	tail neuron		WBbt:0006759

1 Genes

WormBase Gene ID	Gene Name	Sequence Name	Organism
WBGene00018285	smk-1	F41E6.4	Caenorhabditis elegans

0 Life Stages