| Pattern | All five tagged alleles showed expression in two areas: the distal germline and spermatheca. Signal in the spermatheca was strongest at the membrane but also sometimes visible at a lower level in spermathecal nuclei. Expression in the distal germline was present in both sexes and strongest in the distal-most ~20 rows of germ cells, becoming weaker more proximally. Germ cells in the distal-most gonad showed membrane staining similar to that seen with antibodies to the extracellular domain of GLP-1 (e.g. Crittenden et al., 1994); in addition, staining in the nucleus was detected, but at a low level compared to membrane staining. Expression in L4 and adult animals was not observed outside the gonad or in the male somatic gonad (although we cannot exclude the possibility of expression below our threshold of detection). We also examined pre-gastrulation embryos and observed both membrane and nuclear expression of tagged GLP-1, in a pattern consistent with GLP-1 expression in the AB cell lineage, as reported previously (Evans et al., 1994; Priess, 2005). | Primary Identifier | Expr14939 |
| Definition | Name | Synonym | Primary Identifier |
|---|---|---|---|
| cell line which early in development becomes differentiated from the remaining somatic cell line, and alone has the potential to undergo meiosis and form gametes. | germ line | germline | WBbt:0005784 |
| A cell that is the AB blast cell or is a lineal descendant of AB. | AB lineage cell | WBbt:0008611 |
| WormBase Gene ID | Gene Name | Sequence Name | Organism |
|---|---|---|---|
| WBGene00001609 | glp-1 | F02A9.6 | Caenorhabditis elegans |
